Photoacoustic tomography through a whole adult human skull with a photon recycler

Nie, Liming; Cai, Xiaoxiao; Maslov, Konstantin; Garcia-Uribe, Alejandro; Anastasio, Mark A.; Wang, Lihong V.

doi:10.1117/1.jbo.17.11.110506

Cited by 112 publications

(100 citation statements)

References 16 publications

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“…Moreover, alternative RSFC signal processing methods such as independent component analysis (ICA) can be explored to complement and cross-validate the seed-based approach (41). Translation of fcPAT to large animals and humans is also possible, as photoacoustic techniques have been used successfully to image through monkey (42) and human skulls (43). Due to its low costs compared with fMRI, fcPAT should enable many laboratories that previously did not consider functional neuroimaging to contribute further to ongoing studies of human disease.…”

Section: Discussionmentioning

confidence: 99%

High-resolution photoacoustic tomography of resting-state functional connectivity in the mouse brain

Avanaki

Xia

Wan

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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The increasing use of mouse models for human brain disease studies presents an emerging need for a new functional imaging modality. Using optical excitation and acoustic detection, we developed a functional connectivity photoacoustic tomography system, which allows noninvasive imaging of resting-state functional connectivity in the mouse brain, with a large field of view and a high spatial resolution. Bilateral correlations were observed in eight functional regions, including the olfactory bulb, limbic, parietal, somatosensory, retrosplenial, visual, motor, and temporal regions, as well as in several subregions. The borders and locations of these regions agreed well with the Paxinos mouse brain atlas. By subjecting the mouse to alternating hyperoxic and hypoxic conditions, strong and weak functional connectivities were observed, respectively. In addition to connectivity images, vascular images were simultaneously acquired. These studies show that functional connectivity photoacoustic tomography is a promising, noninvasive technique for functional imaging of the mouse brain.fcPAT | RSFC | mouse brain functional imaging | hyperoxia | hypoxia

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Section: Discussionmentioning

confidence: 99%

High-resolution photoacoustic tomography of resting-state functional connectivity in the mouse brain

Avanaki

Xia

Wan

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

278

250

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“…It is a new emerging imaging technique that permits ample contrasts on account of the chromophore absorption 11. Recently, PAT has been widely used on both structural and functional imaging of tumor,12 microvessels,13 brain functions,14 vasculature related diseases,15 and other applications 16, 17. Due to these advantages, we employed fast PAT to monitor the damage region of the mouse brain and its recovery with both skull and scalp intact.…”

Section: Introductionmentioning

confidence: 99%

In Vivo Photoacoustic Imaging of Brain Injury and Rehabilitation by High‐Efficient Near‐Infrared Dye Labeled Mesenchymal Stem Cells with Enhanced Brain Barrier Permeability

Chen

et al. 2017

Advanced Science

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Stem cell migration and interaction with pathology are critical to understand the complexity and status of disease recovery progress. However, the dynamic visualization still remains a great challenge due to imaging technical limitation, cell labeling difficulty, or blood–brain barrier (BBB). Herein, fast photoacoustic tomography (PAT) with optical molecular probes is applied to noninvasively monitor traumatic brain injury (TBI) and its rehabilitation. The vascular distribution and TBI hemorrhage are clearly imaged, longitudinally monitored, and quantified. Bone mesenchymal stem cells (BMSCs) labeled with modified Prussian blue particles (PBPs), excellent near‐infrared dyes and photoacoustic contrasts, are intravenously injected to the mice for improved observation and efficient therapy. BMSCs are demonstrated to be capable of overcoming BBB with enhanced delivery of PBPs to the brain parenchyma. Notably, the versatile BMSCs are observed by PAT to home to the damage region and repair the ruptured vasculature. Moreover, the wound treated by BMSCs exhibits much faster recovery speed than that without treatment. These findings can potentially provide a new noninvasive and high‐resolution approach to image TBI, monitor recovery process, and especially trace BMSCs. This study will stimulate extensive researches on brain diseases and provide promising strategies of dye labeled BMSCs in regenerative medicine.

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“…By combining features of photonics with ultrasound, photoacoustic technique improves both imaging and diagnostic potentialities of single methods [12][13][14][15][16] . Photoacoustic imaging of the brain has the potential to elucidate multiple questions in neurobiology, however, the skullcap that naturally protects the encephalon, dramatically limits both the photonic and ultrasonic tissue penetration [17][18][19] . Moreover, bones promote scattering of both light and sound resulting in loss of sensitivity and image aberrations [17][18] .…”

Section: Introductionmentioning

confidence: 99%

“…Photoacoustic imaging of the brain has the potential to elucidate multiple questions in neurobiology, however, the skullcap that naturally protects the encephalon, dramatically limits both the photonic and ultrasonic tissue penetration [17][18][19] . Moreover, bones promote scattering of both light and sound resulting in loss of sensitivity and image aberrations [17][18] . As a consequence, brain ultrasonic and photoacoustic imaging can be easily performed on neonate animals prior to ossification 20 , but the deep anatomy and physiology of the adult brain are clearly accessible only after craniotomy 21,22 .…”

Section: Introductionmentioning

confidence: 99%

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Non-invasive Parenchymal, Vascular and Metabolic High-frequency Ultrasound and Photoacoustic Rat Deep Brain Imaging

Giustetto

Filippi

Castano

et al. 2015

JoVE

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Photoacoustics and high frequency ultrasound stands out as powerful tools for neurobiological applications enabling high-resolution imaging on the central nervous system of small animals. However, transdermal and transcranial neuroimaging is frequently affected by low sensitivity, image aberrations and loss of space resolution, requiring scalp or even skull removal before imaging. To overcome this challenge, a new protocol is presented to gain significant insights in brain hemodynamics by photoacoustic and high-frequency ultrasounds imaging with the animal skin and skull intact. The procedure relies on the passage of ultrasound (US) waves and laser directly through the fissures that are naturally present on the animal cranium. By juxtaposing the imaging transducer device exactly in correspondence to these selected areas where the skull has a reduced thickness or is totally absent, one can acquire high quality deep images and explore internal brain regions that are usually difficult to anatomically or functionally describe without an invasive approach. By applying this experimental procedure, significant data can be collected in both sonic and optoacoustic modalities, enabling to image the parenchymal and the vascular anatomy far below the head surface. Deep brain features such as parenchymal convolutions and fissures separating the lobes were clearly visible. Moreover, the configuration of large and small blood vessels was imaged at several millimeters of depth, and precise information were collected about blood fluxes, vascular stream velocities and the hemoglobin chemical state. This repertoire of data could be crucial in several research contests, ranging from brain vascular disease studies to experimental techniques involving the systemic administration of exogenous chemicals or other objects endowed with imaging contrast enhancement properties. In conclusion, thanks to the presented protocol, the US and PA techniques become an attractive noninvasive performance-competitive means for cortical and internal brain imaging, retaining a significant potential in many neurologic fields.

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Photoacoustic tomography through a whole adult human skull with a photon recycler

Cited by 112 publications

References 16 publications

High-resolution photoacoustic tomography of resting-state functional connectivity in the mouse brain

High-resolution photoacoustic tomography of resting-state functional connectivity in the mouse brain

In Vivo Photoacoustic Imaging of Brain Injury and Rehabilitation by High‐Efficient Near‐Infrared Dye Labeled Mesenchymal Stem Cells with Enhanced Brain Barrier Permeability

Non-invasive Parenchymal, Vascular and Metabolic High-frequency Ultrasound and Photoacoustic Rat Deep Brain Imaging

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