Frontiers of Biological Energetics 1978
DOI: 10.1016/b978-0-12-225402-4.50009-7
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Photoaffinity Labels for the Study of Cytochrome C and Phospholipid Interactions With Cytochrome C Oxidase

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Cited by 6 publications
(6 citation statements)
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“…The picture emerging for the sidedness of the protein components is in agreement with the three-dimensional reconstruction of the enzyme obtained by R. Henderson (Cambridge) [24,2.5] and may be further specified by the method of photoaffinity labelling [26] with synthetic arylazidophospholipids reported by R. Bisson (Padua) [27,28]. Here 'deep' and 'shallow' probes provide tools for tracing the membrane domains of this [29] and other membrane proteins, among them cytochrome bs and the mitochondrial enzymes succinate ubiquinone reducatase, succinate cytochrome c reductase and ATP synthase.…”
supporting
confidence: 68%
“…The picture emerging for the sidedness of the protein components is in agreement with the three-dimensional reconstruction of the enzyme obtained by R. Henderson (Cambridge) [24,2.5] and may be further specified by the method of photoaffinity labelling [26] with synthetic arylazidophospholipids reported by R. Bisson (Padua) [27,28]. Here 'deep' and 'shallow' probes provide tools for tracing the membrane domains of this [29] and other membrane proteins, among them cytochrome bs and the mitochondrial enzymes succinate ubiquinone reducatase, succinate cytochrome c reductase and ATP synthase.…”
supporting
confidence: 68%
“…The present work shows that nitrophenyl azidophospholipids can be used advantageously in protein-topology studies. Indeed we have already reported preliminary studies of well-characterized membrane proteins such as cytochrome b5 (Bisson et al, 1979b), cytochrome c oxidase (Bisson et al, 1979a) and the mitochondrial and (Na+ + K+)activated ATPases (Montecucco et al, 1979(Montecucco et al, , 1980. Nevertheless some points need further investigation.…”
Section: Discussionmentioning
confidence: 99%
“…Its activity measured with an NADH-coupled system ranged from 10.4 to 12.5 units and its lipid/protein ratio from 28 to 37. (Nat + K+)-activated ATPase from pig kidney, cytochrome c oxidase and oligomycinsensitive ATPase from bovine mitochondria were prepared and assayed as reported previously (Montecucco et al, 1979(Montecucco et al, , 1980Bisson et al, 1979a). Protein concentration was determined by the method of Lowry et al (1951) and lipids either by the method of Bartlett (1959) or by g.l.c.…”
Section: Methodsmentioning
confidence: 99%
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“…The use of hydrophobic reagents, such as adamantane diazirine [38] and arylazidophospholipids [39,401, has been of invaluable help in mapping subunit 111. These reagents are lipophilic and thus react with bilayer-intercalated parts of the most hydrophobic subunits, including subunit 111.…”
Section: Chemical Modification Ojsuhunit 111mentioning
confidence: 99%