Photobiomodulation alters matrix protein activity in stressed fibroblast cells in vitro

Abstract

A balance is maintained between matrix synthesis and degradation, and a prolonged increase in matrix metalloproteinases (MMPs) affects healing. Photobiomodulation (PBM) speeds up healing and alters wound environment. The study aimed to determine changes in protein and gene expression of collagen type 1 (Col-I), MMP-3 and -9 and TIMP-1 in fibroblasts irradiated at 660 or 830 nm. Commercially purchased human skin fibroblast cells were modeled into five groups namely, normal, normal wounded, diabetic wounded, hypoxic wounded and diabetic hypoxic wounded. Control cells were sham irradiated. Laser irradiation was conducted at 660 or 830 nm (108/or 94 mW, 9.1 cm , 420/or 483 s) with 5 J/cm . Forty-eight hours post-irradiation, protein expression of TIMP-1, MMP-3, -9 and Col-I was determined by flow cytometry and immunofluorescence, and gene expression by real-time RT-PCR. There was an increase in TIMP-1 and Col-I, and a decrease in MMP-3 and -9, as well as an alteration in mRNA expression of MMP3, MMP9, TIMP1 and COL1A1 in irradiated cells. Due to the responsiveness of the diabetic hypoxic wounded model, the findings propose this model as appropriate for wound healing studies and suggest that PBM promotes the remodeling phase of wound healing by decreasing matrix degradation and upregulating synthesis.

Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells

“…Proliferation results ( figure 7 ) indicate that cells treated with irradiation and PS only had no significant impact when compared with their control cells receiving no treatment. Although previous studies established that irradiation at wavelengths between 630 and 800 nm using low energy densities of 10 J cm −2 had a proliferative effect on various cell lines [ 56 – 58 ], it was noted that irradiation had no significant effect on lung CSCs. This is in accordance with research stating that CSCs or stem cells have a decreased or quiescent metabolism [ 59 ], which ultimately impacts the metabolic rate and the effect irradiation has on lung CSCs, also considering the fluence and wavelength used to irradiate the cells.…”

Photobiomodulation as an Adjunctive Treatment to Physiotherapy for Reduction of Anterior Knee Pain in Combat Soldiers: A Prospective, Double‐Blind, Randomized, Pragmatic, Sham‐Controlled Trial

“…PBM may accelerate healing in this condition through improvement in tendon and ligament biomechanical properties including enhancement of microvascular capillary flow and tissue perfusion [44][45][46][47]. On the cellular level, PBM has been shown to stimulate proliferation, migration, and increase synthesis of collagen particularly in stressed tenocytes [48][49][50][51], other stressed fibroblasts [52], and serum-starved smooth muscle cells [53]. These effects are associated with stimulation of mitochondrial function by PBM, as evidenced by increased mitochondrial membrane potential [15,16] preventing cell deterioration and apoptosis in spite of stressed conditions [54].…”

Cell Adhesion Molecules Are Mediated by Photobiomodulation at 660 nm in Diabetic Wounded Fibroblast Cells

“…Fibril formation during wound healing is primarily carried out by collagen type I and III. Previous studies in the same cells have shown an increase in collagen type I secretion, as well as its up-regulation in response to irradiation at 660 nm with 5 J/cm 2 [ 1 , 61 ]. Carvalho et al [ 62 ] and Colombo et al [ 63 ] both found increased collagen and improved healing in wounded rat models in response to PBM.…”