Photobiomodulation and Growth Factors in Dentistry: A Systematic Review

Dipalma, Gianna; Inchingolo, Angelo Michele; Patano, Assunta; Palumbo, Irene; Guglielmo, Mariafrancesca; Trilli, Irma; Netti, Anna; Ferrara, Irene; Viapiano, Fabio; Inchingolo, Alessio Danilo; Favia, Gianfranco; Dongiovanni, Leonardo; Palermo, Andrea; Inchingolo, Francesco; Limongelli, Luisa

doi:10.3390/photonics10101095

Cited by 3 publications

(1 citation statement)

References 68 publications

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“…In relation to laser therapy, there are several application protocols, varying in their wavelength and energy density, so there is still no consensus protocol, but several studies that used a protocol similar to the one in our study (wavelength 830 nm, optical output power 30 mW, energy density 6.2 J/cm 2 ), which revealed benefits to the healing process and faster bone tissue formation [101][102][103][104][105][106]. There is still a need to expand the studies related to the use of laser photobiomodulation in order to better clarify the possible protocols and their actions.…”

Section: Discussionmentioning

confidence: 79%

Photobiomodulation Therapy Improves Repair of Bone Defects Filled by Inorganic Bone Matrix and Fibrin Heterologous Biopolymer

Vigliar,

Marega,

Duarte

et al. 2024

Bioengineering

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Biomaterials are used extensively in graft procedures to correct bone defects, interacting with the body without causing adverse reactions. The aim of this pre-clinical study was to analyze the effects of photobiomodulation therapy (PBM) with the use of a low-level laser in the repair process of bone defects filled with inorganic matrix (IM) associated with heterologous fibrin biopolymer (FB). A circular osteotomy of 4 mm in the left tibia was performed in 30 Wistar male adult rats who were randomly divided into three groups: G1 = IM + PBM, G2 = IM + FB and G3 = IM + FB + PBM. PBM was applied at the time of the experimental surgery and three times a week, on alternate days, until euthanasia, with 830 nm wavelength, in two points of the operated site. Five animals from each group were euthanized 14 and 42 days after surgery. In the histomorphometric analysis, the percentage of neoformed bone tissue in G3 (28.4% ± 2.3%) was higher in relation to G1 (24.1% ± 2.91%) and G2 (22.2% ± 3.11%) at 14 days and at 42 days, the percentage in G3 (35.1% ± 2.55%) was also higher in relation to G1 (30.1% ± 2.9%) and G2 (31.8% ± 3.12%). In the analysis of the birefringence of collagen fibers, G3 showed a predominance of birefringence between greenish-yellow in the neoformed bone tissue after 42 days, differing from the other groups with a greater presence of red-orange fibers. Immunohistochemically, in all experimental groups, it was possible to observe immunostaining for osteocalcin (OCN) near the bone surface of the margins of the surgical defect and tartrate-resistant acid phosphatase (TRAP) bordering the newly formed bone tissue. Therefore, laser photobiomodulation therapy contributed to improving the bone repair process in tibial defects filled with bovine biomaterial associated with fibrin biopolymer derived from snake venom.

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Section: Discussionmentioning

confidence: 79%

Photobiomodulation Therapy Improves Repair of Bone Defects Filled by Inorganic Bone Matrix and Fibrin Heterologous Biopolymer

Vigliar,

Marega,

Duarte

et al. 2024

Bioengineering

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Enhancement of the angiogenic differentiation in the periodontal ligament stem cells using fibroblast growth factor 2 and photobiomodulation: An in vitro investigation

Atarbashi‐Moghadam,

Mahmoudian,

Taghipour

et al. 2024

Photochem & Photobiology

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This study aims to evaluate and compare the effect of fibroblastic growth factor 2 (FGF‐2) and photobiomodulation, solely or in combination, in angiogenic differentiation of human periodontal ligament stem cells (hPDLSCs). The study comprises the following groups: control group (hPDLSCs only), FGF‐2 (50 ng/mL) group, two photobiomodulation groups with a 4 J/cm2 energy density of 808 nm diode laser (1‐Session or 2‐Session), and two groups with the combination of each 1‐Session or 2‐Session photobiomodulation with FGF‐2 (50 ng/mL). The 4′,6‐diamidino‐2‐phenylindole (DAPI) staining, and Methyl Thiazolyl Tetrazolium (MTT) assay were undertaken on days 2, 4, and 6. Quantitative Real‐time Polymerase Chain Reaction (RT‐qPCR) analysis on days 2, 4, 6, 8, and 11 was conducted to investigate VEGF‐A and ANG‐I genes. Coherently, the results of the DAPI and MTT showed the Laser (2‐Session) group had higher cell viability than others on day 6. All groups demonstrated a growth pattern in the expression of VEGF‐A and ANG‐I from day 2 to 8 and, afterward, a significant downgrowth to day 11 (p < 0.05). The most amounts of expression of VEGF‐A and ANG‐I on day 8 were seen in the Laser (2‐Session) group. Two‐time application of photobiomodulation using a diode laser with 808 nm wavelength after 2 and 4 days of cell seeding can be associated with higher cell viability and angiogenic differentiation of hPDLSCs compared to the one‐time application of photobiomodulation and administration of FGF‐2.

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Evaluating the effectiveness of low-level laser therapy in reducing local anesthesia injection pain in children

Alafandy,

Almourabet

2024

Preprint

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Aim The aim of this study was to evaluate the efficacy of low-level laser therapy (LLLT) in reducing the pain of needle injection during local anesthesia in children. Materials and Methods Twenty cooperative healthy kids participated in this research; their ages were between 8-12 years old. In laser group (LG) LLLT diode laser (635 nm; continuous mode; 100 mW; 48.0 J/cm2) was applied buccally in one side of the mandibula or maxilla in contact with the soft tissue for 120 second. While in the contralateral side 20% Benzocaine Gel topical anesthetic was applied for the same time and this was the control group (CG). Then a short needle was inserted in each side and the pain was evaluated for each side separately using the Wong-Baker Faces Pain Rating Scale (PRS). Non-parametric analysis was applied and data were analyzed for statistical significance (p < 0.05). Results The total cases were twenty, (10 in the maxilla and 10 in the mandible). The "no pain" and "severe pain" rates in the PRS were 41.7% and 3.3% for the LG and 21.7% and 11.7% for the CG, respectively, during injection. The mean pain degree was 2.6 in CG while it was 1.2 in LG. Wilcoxon analysis revealed a statistically significant difference between LG and CG (P=0.001<0.05). While there was no significant difference in evaluating pain degree according to the order of technique, gender, place of injection, previous dental experiment, and behavior assessment in both methods. Conclusion Administration of LLLT diode laser (635 nm) can be considered for decreasing pain of injection in children and it is more effective than 20% Benzocaine topical anesthetic gel, regardless the order of technique, gender, place of injection, previous dental experiment, and behavior assessment.

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Photobiomodulation and Growth Factors in Dentistry: A Systematic Review

Cited by 3 publications

References 68 publications

Photobiomodulation Therapy Improves Repair of Bone Defects Filled by Inorganic Bone Matrix and Fibrin Heterologous Biopolymer

Photobiomodulation Therapy Improves Repair of Bone Defects Filled by Inorganic Bone Matrix and Fibrin Heterologous Biopolymer

Enhancement of the angiogenic differentiation in the periodontal ligament stem cells using fibroblast growth factor 2 and photobiomodulation: An in vitro investigation

Evaluating the effectiveness of low-level laser therapy in reducing local anesthesia injection pain in children

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