Photobiomodulation and photodynamic therapy applied after electrocauterization for skin healing optimization in rats

Abstract

Photobiomodulation-PBM and Photodynamic Therapy-PDT have been used to induce healing. However, the effects of these therapies on skin-lesions induced by electrocautery are unknown, aiming at more favorable clinical and esthetic results. Electrocauterization was done in 78-female Wistar-rats using a system that includes an electrocautery and red-LED. The groups were: No injury, Injury, Injury + ALA (topical 5-aminolevulinic acid application), Injury + LED and Injury + ALA + LED (topical ALA application followed by photoactivation with LED). After 2nd, 7th and 14th days post-injury, immuno-histomorphometric analyses (inflammatory infiltrate, blood vessels, fibroblasts, eschar/epidermal thickness, IL-10 and VEGF) and biochemical assays of MPO (neutrophil), NAG (macrophage), nitrite, DCF (H 2 O 2 ), carbonyl (membrane's damage), sulfhydryl (membrane's integrity), SOD, GSH, hydroxyproline and re-epithelialization area were performed. The Injury + LED and Injury + ALA + LED groups controlled inflammation and oxidative stress, favoring angiogenesis, fibroblasts proliferation and collagen formation.

Positive effects of low‐dose photodynamic therapy with aminolevulinic acid or its methyl ester in skin rejuvenation and wound healing: An update

“…This indicated that low‐dose ALA‐PDT could regulate the wound healing process by regulating the polarization of macrophages. Lately, the effects of low‐dose ALA‐PDT on the skin healing process after electrocauterization in Wistar‐rats was investigated [68]. This study showed that lower number of inflammatory cells after 2nd day, greater IL‐10 and higher concentration of antioxidants (SOD and GSH) after 7th day after treated with 3% ALA and 36 J/cm 2 light dose in the wound caused by electrocauterization.…”