Photobiomodulation and photodynamic therapy-induced switching of autophagy and apoptosis in human dermal fibroblasts

Pevná, Viktória; Horváth, Denis; Wagnières, Georges; Huntošová, Veronika

doi:10.1016/j.jphotobiol.2022.112539

Cited by 13 publications

(11 citation statements)

References 27 publications

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“…Confocal fluorescence images were collected with an LSM700 confocal microscope (ZEISS, Oberkochen, Germany) as described previously [ 19 ]. The analysis was conducted with ZEN2011 software, version 1.0 (ZEISS).…”

Section: Methodsmentioning

confidence: 99%

“…In other words, the stimulation of mitochondrial signaling pathways, for example, autophagy, by PBM is caused by the activation of complex IV of the mitochondrial electron transport chain (cytochrome c oxidase). Our recent studies have shown that PBM has effects on healthy and cancer cells [ 19 , 20 ]. Damaged mitochondria were remodeled by PBM in a very short time (within 4 h) after the irradiation of cells [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…In our previous study, we showed that combined treatment with PBM and PDT stimulated autophagy and apoptosis in human dermal fibroblasts. However, these effects were less pronounced in noncancerous cell lines than in cancer cells [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, PBM has been shown to influence PDT by increasing the uptake of metal-based photosensitisers [ 40 ] and Photogem ® [ 41 ] by target cells and the homogenised production of protoporphyrin IX in vitro and in vivo [ 42 ]. Furthermore, the induction of autophagy in cancer cells by PBM led to an improvement of anti-cancer PDT in glioblastoma [ 19 ] and breast cancer cells [ 43 ].…”

Section: Introductionmentioning

confidence: 99%

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The Remodulation of Actin Bundles during the Stimulation of Mitochondria in Adult Human Fibroblasts in Response to Light

Olejárová,

Horváth,

Huntošová

2023

Pharmaceutics

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β-actin belongs to cytoskeletal structures that change dynamically in cells according to various stimuli. Human skin can be considered as an organ that is very frequently exposed to various stress factors, of which light plays an important role. The present study focuses on adult human fibroblasts exposed to two types of light stress. Orange light with a wavelength of 590 nm was used here to stimulate the photosensitizer localized in the cells as a residual dose of photodynamic therapy (PDT). On the other hand, near-infrared light with a wavelength of 808 nm was considered for photobiomodulation (PBM), which is often used in healing processes. Confocal fluorescence microscopy was used to observe changes in intercellular communication, mitochondrial structures, and cytoskeletal dynamics defined by the remodulation of β-actin of fibroblasts. The number of β-actin bundles forming spherical structures was detected after light exposure. These structures as β-actin oligomers were confirmed with super-resolution microscopy. While PDT led to the disintegration of actin oligomers, PBM increased their number. The interaction of β-actin with mitochondria was observed. The combination of PDT and PBM treatments is important to minimize the side effects of cancer treatment with PDT on healthy cells, as shown by the cell metabolism assay in this work. In this work, β-actin is presented as an important parameter that changes and is involved in the response of cells to PDT and PBM.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Remodulation of Actin Bundles during the Stimulation of Mitochondria in Adult Human Fibroblasts in Response to Light

Olejárová,

Horváth,

Huntošová

2023

Pharmaceutics

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“…The response of the cells to PDT was determined 24 h after PDT. The optimization of the therapeutic protocol was based on our previous publication, in which the control measurements were performed without hypericin and light [ 12 , 24 ]. No significant effects of light application were observed in the studied cells.…”

Section: Methodsmentioning

confidence: 99%

Spheroidal Model of SKBR3 and U87MG Cancer Cells for Live Imaging of Caspase-3 during Apoptosis Induced by Singlet Oxygen in Photodynamic Therapy

et al. 2022

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Aspects related to the response of cells to photodynamic therapy (PDT) have been well studied in cell cultures, which often grow in monolayers. In this work, we propose a spheroidal model of U87MG and SKBR3 cells designed to mimic superficial tumor tissue, small spheroids (<500 µm) suitable for confocal fluorescence microscopy, and larger spheroids (>500 µm) that can be xenografted onto quail chorioallantoic membrane (CAM) to study the effects of PDT in real time. Hypericin was used as a model molecule for a hydrophobic photosensitizer that can produce singlet oxygen (1O2). 1O2 production by hypericin was detected in SKBR3 and U87MG spheroid models using a label-free technique. Vital fluorescence microscopy and flow cytometry revealed the heterogeneity of caspase-3 distribution in the cells of the spheroids. The levels of caspase-3 and apoptosis increased in the cells of spheroids 24 h after PDT. Lactate dehydrogenase activity was evaluated in the spheroids as the most reliable assay to detect differences in phototoxicity. Finally, we demonstrated the applicability of U87MG spheroids on CAM in photodiagnostics. Overall, the variability and applicability of the prepared spheroid models were demonstrated in the PDT study.

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Wearable photobiomodulation halts thyroid cancer growth by leveraging thyroid photosensitivity

Zhao,

Fu,

Tian

et al. 2024

Bioengineering & Transla Med

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With papillary thyroid carcinoma (PTC) rates rising significantly, concerns about conventional treatments like thyroidectomy and radiotherapy highlight the need for non‐invasive options. Our study explores photobiomodulation therapy (PBMT), which uses specific light wavelengths to evoke cellular responses in PTC treatment. Our research utilized a custom‐designed optical system to investigate PBMT, finding that blue light at a wavelength of 465 nm can safely and effectively inhibit the proliferation of the TPC‐1 PTC cell line by inducing cell cycle arrest. Additionally, we developed a wirelessly powered wearable PBMT device, which is equipped with an advanced light delivery system that ensures precise and consistent dosage. This device designed for optimal patient comfort, effectively suppressed tumor growth in mouse models without adverse effects. PBMT indicates thyroid tissue's light responsiveness as a non‐visual organ. Our study's innovative approach integrates the disciplines of oncology, biophysics, and medical device technology, thereby advancing the treatment paradigms for PTC. This interdisciplinary bridge not only highlights our groundbreaking findings but also paves the way for future research in cancer therapy and photomedicine.

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Photobiomodulation and photodynamic therapy-induced switching of autophagy and apoptosis in human dermal fibroblasts

Cited by 13 publications

References 27 publications

The Remodulation of Actin Bundles during the Stimulation of Mitochondria in Adult Human Fibroblasts in Response to Light

The Remodulation of Actin Bundles during the Stimulation of Mitochondria in Adult Human Fibroblasts in Response to Light

Spheroidal Model of SKBR3 and U87MG Cancer Cells for Live Imaging of Caspase-3 during Apoptosis Induced by Singlet Oxygen in Photodynamic Therapy

Wearable photobiomodulation halts thyroid cancer growth by leveraging thyroid photosensitivity

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