Photobiomodulation at 830 nm Stimulates Migration, Survival and Proliferation of Fibroblast Cells

Abstract

Purpose Photobiomodulation (PBM) promotes diabetic wound healing by favoring cell survival and proliferation. This study aimed to investigate the potential of PBM in stimulating cellular migration, viability, and proliferation using the transforming growth factor-β1 (TGF-β1)/Smad signaling pathway. Methods The study explored the in vitro effects of near infrared (NIR) light on cell viability (survival) and proliferation as well as the presence of TGF-β1, phosphorylated TGF‐β receptor type I (pTGF-βR1) and phosphorylated mothers against decapentaplegic‐homolog (Smad)‐2/3 (p-Smad2/3) in different fibroblast cell models. Results Results show a significant increase in cellular migration in wounded models, and increased viability and proliferation in irradiated cells compared to their respective controls. An increase in the presence of TGF-β1 in the culture media, a reduction in pTGF-βR1 and a slight presence of p-Smad2/3 was observed in the cells. Conclusion These findings show that PBM at 830 nm using a fluence of 5 J/cm 2 could induce cell viability, migration and proliferation to favor successful healing of diabetic wounds. This study contributes to the growing body of knowledge on the molecular and cellular effect of PBM and showcases the suitability of PBM at 830 nm in managing diabetic wounds.

Glucose Improves the Efficacy of Photobiomodulation in Changing ATP and ROS Levels in Mouse Fibroblast Cell Cultures

“…Previous studies have reported similar photobiomodulation-induced effects in cell culture, in particular, an increase in ATP [45,49,50] and a decrease in ROS levels (e.g., [51][52][53][54]). Further, many studies have reported that the resultant changes in ATP and ROS levels are rather short-term but nevertheless, beneficial to cell function and survival (see Introduction and Conclusions [2,15,16].…”

Effects of low‐level Er:YAG laser irradiation on proliferation and gene expression in primary gingival fibroblasts isolated from mouse maxilla

“…Via the activation of the MAPK family‐associated protein, TGF‐βRI kinase, TGF‐β transmitted signals to neighboring cells [49]. Exposure to near‐infrared light at 830 nm using a fluence of 5 J/cm 2 increased fibroblast migration, survival, and proliferation via activation of TGF‐β1/Smad signaling [50]. Low‐level laser irradiation is also believed to promote wound healing by activating the Smad pathway via TGF‐β [51].…”

Photobiomodulation (PBM): a therapeutic technique targeting fibroblast cell regeneration and survival in diabetic wounds

“…They noted that both 630 nm and 810 nm irradiation significantly stimulated cell proliferation and migration. In another study, Oyebode and Houreld, (Oyebode and Houreld, 2022), noted a significantly increased human fibroblast cell proliferation and migration when they investigated the potential of PBM at 830 nm in accelerating cell proliferation and migration.…”

“…The differentiation of fibroblasts to myofibroblasts is regulated by different growth factors and the stiffness of the ECM (Sassoli et al, 2016). When Oyebode and Houreld, (Oyebode and Houreld, 2021), used diode lasers at a wavelength of 830 nm and 660 nm at a fluence of 5 J/cm 2 on diabetic human fibroblast cells they reported successful initiation of fibroblast cell differentiation into myofibroblasts at a wavelength of 660 nm than 830 nm. However, they noted better results at a wavelength of 660 nm than at a wavelength of 830 nm.…”