Photobiomodulation effects of pulsed-NIR laser (810 nm) and LED (808 ± 3 nm) with identical treatment regimen on burn wound healing: A quantitative label-free global proteomic approach

Keshri, Gaurav K.; Kumar, Gaurav; Sharma, Manish; Bora, Kiran; Kumar, Bhuvnesh; Gupta, Asheesh

doi:10.1016/j.jpap.2021.100024

Cited by 13 publications

(6 citation statements)

References 52 publications

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“…For instance, although T6 and T9 yielded the same dose (0.3125 J/cm 2 ), the biological response of the cells behaved differently; after 48h, DPSCs cultured in 5% FBS in T6 (0.3 × 10 s) showed a significant increase in the proliferation rate of cells when cells compared to the control untreated group, while after 72 h, cells in the same group T6 showed a significant decrease in the proliferation rate compared to the control untreated group, and cells in group T9 (1 × 3 s) showed a significant increase in the proliferation rate of the treated cells. These current findings, which demonstrate the non-reciprocity effect of laser photobiomodulation on cells, confirm previous reports that demonstrated the nonlinear effect of laser photobiomodulation; even if the energy density is the same (inverse linear relationship between irradiance and time), the changes in biological endpoint will not necessarily be equal [21].…”

Section: Discussionsupporting

confidence: 91%

Potential Effects of Photobiomodulation Therapy on Human Dental Pulp Stem Cells

Al-Asmar,

Abuarqoub,

Ababneh

et al. 2023

Applied Sciences

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Background: A significant clinical technology enabling the use of stem cells in dentistry is Photobiomodulation (PBM). The current study aimed to investigate various PBM treatment variables on human dental pulp stem cell proliferation, survival, and changes in cell cycle progression. Methods: Human third molar samples were collected, and human Dental Pulp Stem Cells (hDPSCs) were isolated, expanded, seeded, and cultured in media containing 0, 1, 5, or 10% FBS. PBM treatments using an 810 nm diode laser at various doses were performed 24, 48, and 72 h after seeding. Cell proliferation and apoptosis were assessed. A two-way ANOVA test was performed among all treatment groups, followed by multiple comparison tests (Tukey’s test) performed between each treated group and its control untreated group. Results: After 24 h, only cells cultured in 10% FBS showed a significant (p < 0.005) increase in the proliferation rate of cells treated with T7 (0.4 W × 10 s). After 48 h, hDPSCs cultured in both 5% and 10% FBS showed a significant (p < 0.005) increase in the proliferation rate of cells treated with T7 (0.4 W × 10 s) as compared to the untreated control. After 72 h, only cells cultured in 10% FBS showed a significant increase in the proliferation rate of the cells treated with T9 (1 W × 3 s) (p < 0.005) and T7 (0.4 W × 10 s) (p < 0.00005). Conclusions: Low-power laser therapy at a wavelength of 810 nm induced hDPSC proliferation at the following parameters: power output 0.4 W, irradiance 0.5 W/cm2, fluence 0.416 J/cm2, exposure time 10 s.

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Section: Discussionsupporting

confidence: 91%

Potential Effects of Photobiomodulation Therapy on Human Dental Pulp Stem Cells

Al-Asmar,

Abuarqoub,

Ababneh

et al. 2023

Applied Sciences

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“…Different approaches have been used to treat diabetic wounds, and when PBM is appropriately used, it elicits biostimulatory effects that speed up the healing process. PBM is principally a wavelength-dependent procedure involving photon-tissue interfaces [21].…”

Section: Discussionmentioning

confidence: 99%

Effect of photobiomodulation at 830 nm on gene expression correlated with JAK/STAT signalling in wounded and diabetic wounded fibroblasts in vitro

Leyane,

Jere,

Houreld

2023

Journal of Biophotonics

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Treatment of chronic diabetic wounds is an ongoing socio‐economic challenge. Dysregulated signalling pathways characterise cells from chronic diabetic wounds. Photobiomodulation (PBM) stimulates healing by eliciting photochemical effects that affect gene regulation. JAK/STAT signalling is a primary signal transduction pathway involved in wound healing. This in vitro study aimed to determine if PBM at 830 nm and a fluence of 5 J/cm2 regulates genes related to JAK/STAT signalling in wounded and diabetic wounded fibroblast cells. A continuous wave diode laser (12.53 mW/cm2) was used to irradiate cells. Forty‐eight hours post‐PBM, RT‐qPCR was used to analyse 84 genes related to JAK/STAT signalling. Five genes were upregulated and four downregulated in wounded cell models, while six genes were downregulated in diabetic wounded models. The results show drastic gene expression differences between wounded and diabetic wounded cell models in response to PBM using 830 nm.

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“…It was reported as a well-tolerated, safe and effective modality to manage OM related complications (12) owing to its anti-inflammatory (13) and antioxidant capabilities (14) . Furthermore, it has the ability to accelerate healing process through activating mitochondrial-ATP production, the local release of growth factors (15,16) , increased proliferation of fibroblasts (17,18) , improving extracellular matrix production, macrophage stimulation or activation of collagen production (19).…”

Section: Introductionmentioning

confidence: 99%

Clinical and Histopathologic Evaluation of the Effect of Laser Photobiomodulation And Epicatechin in the Treatment of Radiation Induced Oral Mucositis in Rat Model

Elewa

El-ezz

Aboulfotoh

2022

Egyptian Dental Journal

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Aim: This study aimed to determine the early-stage anticytotoxic effects of Ga-Al-As diode laser photobiomodulation (LPBM) and Epicatechin (green tea extract) (GT), either alone or combined, when used to treat induced oral mucositis (OM) in rats.Subjects & Methods: Thirty Wistar rats were included; two of them were sacrificed in the beginning (normal mucosa control). Radiotherapy OM was induced by a fractionated course of oral radiotherapy at a rate of 2 Gy/min bilaterally twice weekly for two weeks. On day 6, four animals were sacrificed to confirm OM induction. The remaining animals (24 rats) were equally assigned into four groups: Control group (CG), Laser group (LG), green tea extract group (GG) and combined group (L+G). They continued for 5 successive days in the 3-test groups. Animal sacrifice has then occurred on day 11. The mucositis degree (MD) was clinically evaluated, the histopathological assessment of tissue changes was applied using (H&E) and Monoclonal cytochrome-c antibody.Results: All treated animals showed a statistically significant difference (P-value ≤ 0.05) than those in CG. The lased animals had a better clinical outcome with a faster healing than GT. The best clinical outcome and healing pattern were shown in (L+G). Conclusion:Ga-Al-As diode laser and Epicatechin produced protection against radiotherapy induced OM through their antioxidant and anti-apoptotic activity effects. Better outcomes were noted in favor of LPBM over GT.

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Photobiomodulation effects of pulsed-NIR laser (810 nm) and LED (808 ± 3 nm) with identical treatment regimen on burn wound healing: A quantitative label-free global proteomic approach

Cited by 13 publications

References 52 publications

Potential Effects of Photobiomodulation Therapy on Human Dental Pulp Stem Cells

Potential Effects of Photobiomodulation Therapy on Human Dental Pulp Stem Cells

Effect of photobiomodulation at 830 nm on gene expression correlated with JAK/STAT signalling in wounded and diabetic wounded fibroblasts in vitro

Clinical and Histopathologic Evaluation of the Effect of Laser Photobiomodulation And Epicatechin in the Treatment of Radiation Induced Oral Mucositis in Rat Model

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