Photobiomodulation mechanisms in the kinetics of the wound healing process in rats

Otterço, Albaiza Nicoletti; Andrade, Ana Luiza Dias Leite de; Brassolatti, Patrícia; Pinto, Karina Nogueira Zambone; Araújo, Heloísa Sobreiro Selistre de; Parizotto, Nivaldo Antônio

doi:10.1016/j.jphotobiol.2018.04.010

Cited by 50 publications

(35 citation statements)

References 32 publications

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“…A semiquantitative analysis was performed through pre‐established scores, considering the values from 0 to 4 (Otterço, Andrade, et al, 2018) and 0 to 3 (Otterço, Andrade, et al, 2018) described in Tables 1 and 2. The analysis was blinded in relation to the experimental groups and was performed by two reviewers.…”

Section: Methodsmentioning

confidence: 99%

“…ImageJ software was used for the quantitative analysis of the expression of collagen III and I through the percentage of area pixels marked with the color deconvolution plug‐in with methyl green diaminobenzidine (Otterço, Andrade, et al, 2018). Three images of each slide were captured with 20× magnification.…”

Section: Methodsmentioning

confidence: 99%

“…Within this context, photobiomodulation therapy (PBM) has been gaining prominence in recent decades, characterized by being a form of phototherapy that involves the application of low power monochromatic and coherent light in several types of wounds, being successful when used to induce healing, repair of complex wounds, and increased local vascularization (Brassolatti, de Andrade, Bossini, Otterço, & Parizotto, 2018; Hawkins & Abrahamse, 2006; Martignago et al, 2018; Otterço, Andrade, et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…The literature shows that the use of PBM in the treatment of burns, especially in the early stages of the process promotes better healing and a shorter tissue repair time through the antioxidant effects provided by the therapy, such as the ability to modulate the intensity of the inflammatory infiltrate and to stimulate the production of macrophages and synthesis of collagen (Avci et al, 2013; Brassolatti et al, 2016). In addition, recent studies have demonstrated that PBM also stimulates subsequent steps in the repair process, assisting in the formation of granulation tissue through stimulation in fibroblast synthesis and formation of new vessels and maturation of type III collagen in type I (Fiório & Albertini, 2014; Martignago et al, 2018; Otterço, Andrade, et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Effect of photobiomodulation associated with cell therapy in the process of cutaneous regeneration in third degree burns in rats

Andrade

Brassolatti

Luna

et al. 2020

J Tissue Eng Regen Med

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Due to the complexity involved in the healing process of full thickness burns, the literature looks for alternatives to optimize tissue reconstruction. The objective of this study was to explore the action of photobiomodulation therapy associated with MSCs in the healing process of third degree burns. A total of 96 male Wistar rats were used, distributed in four groups with 24 animals each: Control Group, Laser Group, Cell Therapy Group, and Laser Group and Cell Therapy. The burn was performed with aluminum plate (150 C). We performed analysis of wound contraction, histology, immunohistochemistry, birefringence analysis, and immunoenzymatic assay to evaluate tissue quality. Our results demonstrate that the association of the techniques is able to accelerate the repair process, modulating the inflammatory process, presenting a cutaneous tissue with better quality. Thus, we conclude that the use of photobiomodulation therapy associated with cell therapy is a promising treatment in the repair of total thickness burns.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of photobiomodulation associated with cell therapy in the process of cutaneous regeneration in third degree burns in rats

Andrade

Brassolatti

Luna

et al. 2020

J Tissue Eng Regen Med

View full text Add to dashboard Cite

show abstract

“…The morphometric analysis used the total pixel percentage in each image using the threshold colour. 33,34…”

Section: Immunohistochemistrymentioning

confidence: 99%

Effects of mesenchymal stromal cells on motor function and collagen in the skeletal muscles of rats with type I diabetes

Luna

Russo

Sabadine

et al. 2019

Int J Experimental Path

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Summary The present study aimed to evaluate the effects of mesenchymal stromal cell (MSC) transplantation on motor function and collagen organization in the muscles of rats with type 1 diabetes mellitus. Male Wistar rats were randomly assigned to three groups: control (C), diabetic (DM) and diabetic treated with MSCs (DM‐MSCs). Diabetes was induced by streptozotocin (50 µg/kg). Bone marrow cells were isolated from the tibia and femur. After 10 weeks of DM induction, the DM‐MSC rats received four i.p. injections of MSCs (1 × 106). Ten weeks after MSC transplantation, motor performance was evaluated by the rotarod test and the anterior tibial (TA) muscles were collected for morphometric and quantification of collagen birefringence by polarizing microscopy analysis. Motor performance of the DM group was significantly reduced when compared to the C group and increased significantly in the DM + MSC group. The TA muscle mass was significantly reduced in the DM and DM + MSC groups compared to the C group. The connective tissue increased in the DM group compared to the C group and decreased in the DM + MSC group. The percentage collagen birefringence decreased significantly in the DM group when compared to the C group and increased in the DM + MSC group. Motor performance was positively correlated with collagen birefringence and negatively correlated with percentage of connective tissue. The results indicate that MSC transplantation improves both motor function and the collagen macromolecular organization in type 1 DM.

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Photobiomodulation in diabetic wound healing: A review of red and near‐infrared wavelength applications

Oyebode

Houreld

Abrahamse

2021

Cell Biochemistry & Function

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The development of a painless, non‐invasive, and faster way to diabetic wound healing is at the forefront of research. The complexity associated with diabetic wounds makes it a cause for concern amongst diabetic patients and the world at large. Irradiation of cells generates a photobiomodulatory response on cells and tissues, directly causing alteration of cellular processes and inducing diabetic wound repair. Photobiomodulation therapy (PBMT) using red and near‐infrared (NIR) wavelengths is being considered as a promising technique for speeding up the rate of diabetic wound healing, eradication of pain and reduction of inflammation through the alteration of diverse cellular and molecular processes. This review presents the extent to which the potential of red and NIR wavelengths have been harnessed in PBMT for diabetic wound healing. Important research challenges and gaps are identified and discussed, and future directions mapped out. This review thus provides useful insights and strategies into improvement of PBMT, including its acceptance within the global medical research community.

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Photobiomodulation mechanisms in the kinetics of the wound healing process in rats

Cited by 50 publications

References 32 publications

Effect of photobiomodulation associated with cell therapy in the process of cutaneous regeneration in third degree burns in rats

Effect of photobiomodulation associated with cell therapy in the process of cutaneous regeneration in third degree burns in rats

Effects of mesenchymal stromal cells on motor function and collagen in the skeletal muscles of rats with type I diabetes

Photobiomodulation in diabetic wound healing: A review of red and near‐infrared wavelength applications

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