Photobiomodulation therapy can change actin filaments of 3T3 mouse fibroblast

Magalhães, Ana Carolina de; Guimarães-Filho, Z. O.; Yoshimura, Elisabeth Mateus; Lilge, Lothar

doi:10.1007/s10103-019-02852-y

Cited by 7 publications

(4 citation statements)

References 24 publications

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“…Moreover the reduction of stress fiber-like structures observed in red PBM treated cells may be indicative of motile migrating cells, based on findings showing that cells with highly motility typically display few, thin and more dynamic stress fibers and that stress fiber are not essential for cell migration [57,58]. Our results concerning the impact of red PBM on cytoskeleton assembly properties of NIH/3T3 fibroblasts are in accordance with a very recent paper by de Magalhães and co-workers [59]. However, differently from our findings, these authors did not find any difference between the cell response, in terms of actin filament content, to red and NIR light.…”

Section: Discussionsupporting

confidence: 93%

“…However, differently from our findings, these authors did not find any difference between the cell response, in terms of actin filament content, to red and NIR light. This may be explained, in addition to the different PBM parameters applied, by the difference in cell growth substrate rigidity, i.e., gel in the work by Magalhães et al versus plastic or glass in our work [59][60][61]. Concomitantly, cells irradiated with violet-blue and NIR light exhibited augmented expression of focal adhesion proteins namely vinculin, which appeared mainly aggregated at the end of stress fiber-like structures, and p-FAK which, among other functions, is required for stress fiber formation in fibroblasts [62].…”

Section: Discussionmentioning

confidence: 72%

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In Vitro Evidences of Different Fibroblast Morpho-Functional Responses to Red, Near-Infrared and Violet-Blue Photobiomodulation: Clues for Addressing Wound Healing

et al. 2020

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Although photobiomodulation (PBM) has proven promising to treat wounds, the lack of univocal guidelines and of a thorough understanding of light–tissue interactions hampers its mainstream adoption for wound healing promotion. This study compared murine and human fibroblast responses to PBM by red (635 ± 5 nm), near-infrared (NIR, 808 ± 1 nm), and violet-blue (405 ± 5 nm) light (0.4 J/cm2 energy density, 13 mW/cm2 power density). Cell viability was not altered by PBM treatments. Light and confocal laser scanning microscopy and biochemical analyses showed, in red PBM irradiated cells: F-actin assembly reduction, up-regulated expression of Ki67 proliferation marker and of vinculin in focal adhesions, type-1 collagen down-regulation, matrix metalloproteinase-2 and metalloproteinase-9 expression/functionality increase concomitant to their inhibitors (TIMP-1 and TIMP-2) decrease. Violet-blue and even more NIR PBM stimulated collagen expression/deposition and, likely, cell differentiation towards (proto)myofibroblast phenotype. Indeed, these cells exhibited a higher polygonal surface area, stress fiber-like structures, increased vinculin- and phospho-focal adhesion kinase-rich clusters and α-smooth muscle actin. This study may provide the experimental groundwork to support red, NIR, and violet-blue PBM as potential options to promote proliferative and matrix remodeling/maturation phases of wound healing, targeting fibroblasts, and to suggest the use of combined PBM treatments in the wound management setting.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 72%

In Vitro Evidences of Different Fibroblast Morpho-Functional Responses to Red, Near-Infrared and Violet-Blue Photobiomodulation: Clues for Addressing Wound Healing

et al. 2020

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“…However, in this particular scenario with varying wavelengths on dentin differentiation, we examined photon energy transfer (PET) noted at 810 nm to further assess responses at individual photon energy. This appears to be particularly relevant given the extremely low, efficacious PBM clinical doses and the antagonistic effects at higher doses termed the Arndt‐Schultz dose curve [32, 42, 43]. Following the physical transfer of light energy, its transformation leads to thermal, biochemical or biophysical changes that contribute to the directed PBM therapeutic responses (Figure 2C).…”

Section: Resultsmentioning

confidence: 99%

Thermodynamic basis for comparative photobiomodulation dosing with multiple wavelengths to direct odontoblast differentiation

Young

Maximiano

Arany

2022

Journal of Biophotonics

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Multiple wavelength devices are now available for photobiomodulation (PBM) treatments, but their dosimetry for individual or combinatorial use remains unclear. The present work investigated the effects of 447, 532, 658, 810, 980 and 1064 nm wavelengths on odontoblast differentiation at 10 mW/cm2 using either equal treatment time for conventional fluence (300 seconds for 3 J/cm2) or varying times to adjust for individual wavelength photon fluence (4.6 p.J/cm2). Both 447 and 810 nm significantly increased alkaline phosphatase (ALP) activity, while 1064 nm showed reduced ALP activity at 3 J/cm2. However, ALP induction was significantly improved when equivalent photon fluence dosing was used. Other wavelengths did not show significant changes compared to untreated controls. The data suggest that accounting for wavelength‐specific photon energy transfer during PBM dosing could improve clinical safety and efficacy.

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“…An indirect effect of PBM on the phosphorylation of protein kinase Cδ, which is involved in cell survival, was detected [ 21 ]. Recently, de Magalhaes et al reported that PBM can alter the morphological characteristics of cells and their cytoskeleton [ 22 ]. Mokoena et al showed that α-smooth muscle actin increased in an injured model after stimulation by PBM [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

The Remodulation of Actin Bundles during the Stimulation of Mitochondria in Adult Human Fibroblasts in Response to Light

Olejárová,

Horváth,

Huntošová

2023

Pharmaceutics

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β-actin belongs to cytoskeletal structures that change dynamically in cells according to various stimuli. Human skin can be considered as an organ that is very frequently exposed to various stress factors, of which light plays an important role. The present study focuses on adult human fibroblasts exposed to two types of light stress. Orange light with a wavelength of 590 nm was used here to stimulate the photosensitizer localized in the cells as a residual dose of photodynamic therapy (PDT). On the other hand, near-infrared light with a wavelength of 808 nm was considered for photobiomodulation (PBM), which is often used in healing processes. Confocal fluorescence microscopy was used to observe changes in intercellular communication, mitochondrial structures, and cytoskeletal dynamics defined by the remodulation of β-actin of fibroblasts. The number of β-actin bundles forming spherical structures was detected after light exposure. These structures as β-actin oligomers were confirmed with super-resolution microscopy. While PDT led to the disintegration of actin oligomers, PBM increased their number. The interaction of β-actin with mitochondria was observed. The combination of PDT and PBM treatments is important to minimize the side effects of cancer treatment with PDT on healthy cells, as shown by the cell metabolism assay in this work. In this work, β-actin is presented as an important parameter that changes and is involved in the response of cells to PDT and PBM.

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Photobiomodulation therapy can change actin filaments of 3T3 mouse fibroblast

Cited by 7 publications

References 24 publications

In Vitro Evidences of Different Fibroblast Morpho-Functional Responses to Red, Near-Infrared and Violet-Blue Photobiomodulation: Clues for Addressing Wound Healing

In Vitro Evidences of Different Fibroblast Morpho-Functional Responses to Red, Near-Infrared and Violet-Blue Photobiomodulation: Clues for Addressing Wound Healing

Thermodynamic basis for comparative photobiomodulation dosing with multiple wavelengths to direct odontoblast differentiation

The Remodulation of Actin Bundles during the Stimulation of Mitochondria in Adult Human Fibroblasts in Response to Light

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