Photobiomodulation with red light‐emitting diodes accelerates hepatocyte proliferation through reactive oxygen species/extracellular signal‐regulated kinase pathway

Feng, Rui; Morine, Yuji; Ikemoto, Tetsuya; Imura, Satoru; Iwahashi, Shuichi; Saito, Yu; Shimada, Mitsuo

doi:10.1111/hepr.13182

Cited by 8 publications

(6 citation statements)

References 41 publications

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“…LED irradiation has positive effects on stimulation of neuronal growth, protection of post-ischemia skeletal muscle, protection of cardiomyocytes, acceleration of wound healing, reduction of the in ammatory response, and stimulation of cell proliferation and differentiation [3,5,7,14]. Here, we demonstrated that GLED irradiation also has an effect on the function of HLCs.…”

Section: Discussionmentioning

confidence: 56%

“…Recently, light-emitting diode (LED) irradiation has been used to treat several clinical conditions, including wound healing, pain, bone regeneration and treatment of various diseases and infections [2]. We have reported that LED irradiation can result in various biological effects, such as mouse hepatocyte proliferation and colon cancer cell autophagy [3,4]. The effect of LED irradiation at different wavelengths and energy densities also improves the regenerative capacity of various types of MSC [5,6].…”

Section: Introductionmentioning

confidence: 99%

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Effects of green light-emitting diode irradiation on hepatic differentiation of hepatocyte-like cells generated from human adipose-derived mesenchymal cells

Waki

Saito

Chen

et al. 2023

Preprint

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Background Light-emitting diode (LED) irradiation has been used in the differentiation of mesenchymal stem cells into a variety of cell types. This study investigated the effect of green LED (GLED) irradiation on the differentiation of adipocyte-derived mesenchymal cells into hepatocyte-like cells (HLCs) and the mechanism of its action. Methods HLCs in the hepatocyte maturation phase were irradiated with GLED (520 nm, 21 W/m2, 5 min/day for 10 days). The cells were then assessed for expression of hepatocyte maturity genes and opsin 3 (OPN3), hepatocyte function, viability, apoptosis, and levels of reactive oxygen species (ROS), intracellular adenosine triphosphate (ATP) and calcium ions (Ca2+). Results GLED irradiation increased Alpha-1 antitrypsin and Ornithine transcarbamylase gene expression, promoted Cytochrome P450 3A4 activity and urea synthesis, and elevated intracellular ROS, ATP and Ca2+ levels. OPN3 expression was significantly more upregulated in GLED-irradiated HLCs than in the non-irradiated HLCs. No significant difference in cell viability or apoptosis was observed between GLED-irradiated and non-irradiated HLCs. Conclusions GLED irradiation can promote hepatocyte maturation and functions through OPN3. GLED irradiation also stimulated mitochondrial function via Ca2+/ATP/ROS activation. GLED irradiation has potential to support cell-based transplantation in patients.

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Section: Discussionmentioning

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Effects of green light-emitting diode irradiation on hepatic differentiation of hepatocyte-like cells generated from human adipose-derived mesenchymal cells

Waki

Saito

Chen

et al. 2023

Preprint

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“…PBM modulates the levels of ROS, since ROS can be produced by red light absorption by mitochondria and opsins and also by the increased mitochondrial membrane potential (MMP), which consequently enhances ATP production . Moreover, ROS induced by PBM can generate several responses: promotion of proliferation by the pERK1/2 pathway ; activation of transcription factors and signaling pathways, for example, transforming growth factor‐ β 1 (TGF‐ β 1), which is capable of differentiating human stem cells . Thus, a combination of different cell death/viability/proliferation assays should be performed to better explain this result.…”

Section: Resultsmentioning

confidence: 99%

Combination of Natural Extracts and Photobiomodulation in Keratinocytes Subjected to UVA Radiation

Silva

Machado

Lino-dos-Santos-Franco

et al. 2018

Photochem & Photobiology

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Natural extracts (NE) with antioxidant properties can minimize the effects of photoaging. Photobiomodulation (PBM) has proven to be a useful tool for the modulation of cell metabolism. Here, we investigate the associations of antioxidants with PBM with the aim of promoting skin rejuvenation. We began with standardization of the experimental protocol. Extracts of chamomile, rosemary, blueberry, green tea, figs, pomegranate and nutwood were tested. A custom irradiation system (366 ± 10 nm) was used to simulate sun exposure. A light emitting diode system (640 ± 12.5 nm) was used for PBM. Viability assessments were performed by the (3‐(4,5‐Dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2H‐tetrazolium bromide) MTT assay method. Based on the results, radiant exposure to UVA was defined as 9 and 1 J cm−2 for PBM. Extract concentrations were established on the basis of dark toxicities, which ranged from 0.01% to 0.3%. The data show that PBM is a promising therapy to restore keratinocytes after UVA damage; however, the detailed mechanism and effects require further exploration. Moreover, although the combination of PBM with NE may be a useful strategy, the choice of a NE is challenging, since the working concentration and other properties, such as photosensitivity, may bring about unwanted results.

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“…Different from intense light treatment that heats the treated tissue, PBM is not mediated by a thermal effect on cells . PBM, especially using a red light source, has also been shown to stimulate hepatocyte proliferation and metabolism , fibroblast proliferation, and wound healing . On the other hand, red light PBM has been widely demonstrated to be beneficial for osteoarthritis , pain control , chemotherapy adverse effect management , and wound healing in animal models or human trials .…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis Reveals Anti‐Fibrotic Effects of Blue Light Photobiomodulation on Fibroblasts

et al. 2019

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Background and Objectives This study was aimed at determining the effects of blue light photobiomodulation on primary adult mouse dermal fibroblasts (AMDFs) and the associated signaling pathways. Study Design/Materials and Methods Cultured AMDFs from adult C57BL/6 mice were irradiated by blue light from a light‐emitting diode (wavelength = 463 ± 50 nm; irradiance = 5 mW/cm2; energy density = 4–8 J/cm2). The cells were analyzed using mass spectrometry for proteomics/phosphoproteomics, AlamarBlue assay for mitochondrial activity, time‐lapse video for cell migration, quantitative polymerase chain reaction for gene expression, and immunofluorescence for protein expression. Results Proteomic/phosphoproteomic analysis showed inhibition of extracellular signal‐regulated kinases/mammalian target of rapamycin and casein kinase 2 pathways, cell motility‐related networks, and multiple metabolic processes, including carbon metabolism, biosynthesis of amino acid, glycolysis/gluconeogenesis, and the pentose phosphate pathway. Functional analysis demonstrated inhibition of mitochondrial activities, cell migration, and mitosis. Expression of growth promoting insulin‐like growth factor 1 and fibrosis‐related genes, including transforming growth factor β1 (TGFβ1) and collagen type 1 ɑ2 chain diminished. Protein expression of α‐smooth muscle actin, an important regulator of myofibroblast functions, was also suppressed. Conclusions Low‐level blue light exerted suppressive effects on AMDFs, including suppression of mitochondrial activity, metabolism, cell motility, proliferation, TGFβ1 levels, and collagen I production. Low‐level blue light can be a potential treatment for the prevention and reduction of tissue fibrosis, such as hypertrophic scar and keloids. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.

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Photobiomodulation with red light‐emitting diodes accelerates hepatocyte proliferation through reactive oxygen species/extracellular signal‐regulated kinase pathway

Abstract: Red LED irradiation could accelerate hepatocyte proliferation through the ROS/pERK1/2 pathway. Red LED irradiation might be a potential method to increase hepatocyte cell numbers in vitro and support cell-based transplantation in clinical work.

Cited by 8 publications

References 41 publications

Effects of green light-emitting diode irradiation on hepatic differentiation of hepatocyte-like cells generated from human adipose-derived mesenchymal cells

Effects of green light-emitting diode irradiation on hepatic differentiation of hepatocyte-like cells generated from human adipose-derived mesenchymal cells

Combination of Natural Extracts and Photobiomodulation in Keratinocytes Subjected to UVA Radiation

Proteomic Analysis Reveals Anti‐Fibrotic Effects of Blue Light Photobiomodulation on Fibroblasts

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