Photobiomodulatory effect delivered by low-level laser on dental pulp stem cell differentiation for osteogenic lineage

Sivakumar, Trivandrum T.; Muruppel, Alex Mathews; Joseph, Anna P.; Reshmi, A.; Ramachandran, Rajesh; Nair, Prabha D.; Mohan, Sunil Paramel

doi:10.1007/s41547-019-00066-7

Cited by 10 publications

(5 citation statements)

References 37 publications

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“…Regarding the appearance of calcification nodules, the 940-nm laser group for 50 s (6.4 J/cm 2 ) presented the highest alizarin red values, similar to the results reported by Sivakumar et al [ 47 ], when applying a laser with a double dose of irradiation. However, in contrast, we found a significant increase in calcification nodule formation with a single irradiation dose.…”

Section: Discussionsupporting

confidence: 88%

“…When studying the effect of 808-nm laser on human periodontal ligament stem cells, a significant increase in cell proliferation and osteogenic differentiation (through the expression of RUNX2, Col-1, ALP, and osteonectin) was observed, especially at 1 and 2 J/cm 2 combined with vitamin D [ 46 ]. Similarly, in another study [ 47 ] comparing the effect of a single and double dose 808-nm laser with a control group in DPSC, the double dose irradiation groups were consistent with an increase in calcium and ALP formation compared to the single irradiation group. In addition, osteopontin expression was significantly increased in the double-dose group compared to the single-dose group.…”

Section: Discussionsupporting

confidence: 58%

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Osteoblastic differentiation and changes in the redox state in pulp stem cells by laser treatment

Escobar,

Grajales,

Bendahan

et al. 2024

Lasers Med Sci

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The aim of this study was to determine the effect of low-level laser therapy (LLLT) on cell proliferation, mitochondrial membrane potential changes (∆Ψm), reactive oxygen species (ROS), and osteoblast differentiation of human dental pulp stem cells (hDPSCs). These cells were irradiated with 660- and 940-nm lasers for 5 s, 50 s, and 180 s. Cell proliferation was assessed using the resazurin assay, cell differentiation by RUNX2 and BMP2 expression, and the presence of calcification nodules using alizarin-red S staining. ROS was determined by the dichlorofluorescein-diacetate technique and changes in ∆Ψm by the tetramethylrhodamine-ester assay. Data were analyzed by a Student’s t-test and Mann–Whitney U test. The 940-nm wavelength for 5 and 50 s increased proliferation at 4 days postirradiation. After 8 days, a significant decrease in proliferation was observed in all groups. Calcification nodules were evident in all groups, with a greater staining intensity in cells treated with a 940-nm laser for 50 s, an effect that correlated with increased RUNX2 and BMP2 expression. ROS production and Δψm increased independently of irradiation time. In conclusion, photobiomodulation (PBM) with LLLT induced morphological changes and reduced cell proliferation rate, which was associated with osteoblastic differentiation and increased ROS and Δψm, independent of wavelength and time.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 58%

Osteoblastic differentiation and changes in the redox state in pulp stem cells by laser treatment

Escobar,

Grajales,

Bendahan

et al. 2024

Lasers Med Sci

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“…For further confirmation, 14 days after inducing osteoblast like cells, ARS staining was used to detect osteogenic differentiation by analyzing calcium concentration. Higher ca ++ levels were detected after LLLT in our results which coincide with ( Sivakumar et al, 2019 ) for DPCSs using the same LLLT and ( Wang et al, 2022 ) for PDLSCs using different LLLT.…”

Section: Discussionsupporting

confidence: 87%

“…The handpiece tip was applied from the top at a constant distance from the flask, by 90° angle under the laminar flow in a dark room to prevent interference of other light for 12 s to get 3.6 J/cm 2 . This procedure was done on day one and repeated on day three ( Sivakumar et al, 2019 ). The samples were divided into subgroups (DMEM control groups (C- DPSCs, C- PDLSCs) and DMEM laser groups (L-DPSCs, L-PDLSCs) used for cell viability assessment.…”

Section: Methodsmentioning

confidence: 99%

Photo biomodulation of dental derived stem cells to ameliorate regenerative capacity: In vitro study

Medhat,

El-Zainy,

Fathy

2024

The Saudi Dental Journal

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“…No statistically significant differences were observed between the proliferation rates and the relative productions of mineralized nodules compared to the respective controls, either for hDPSC from normal or inflamed dental pulps Sivakumar et al 19 2019 India…”

Section: Ginani Et Al 17 2018 Brazilmentioning

confidence: 94%

Effect of Low-Level Laser Therapy on Differentiation and Proliferation of Human Dental Pulp Stem Cells: A Systematic Review

Karkehabadi,

Zafari,

Khoshbin

et al. 2023

J Lasers Med Sci

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Introduction: Considering the positive impact of laser treatment on the proliferation of certain cell types, we opted to perform a systematic review aimed at evaluating the effects of laser therapy and photobiomodulation on the proliferation and differentiation of human dental pulp stem cells (hDPSCs). Methods: We included all research studies examining the impact of laser therapy on hDPSCs, without limitations on publication dates or article languages. The major international databases, including PubMed, ISI Web of Science, and Scopus, were searched from inception to April 2022 by the relevant keywords. Results: In total, 1886 studies were identified in the initial search from the mentioned databases and other sources. Finally, 17 relevant studies were included in the present systematic review after removing duplicates and non-relevant articles. The results indicated the useful effect of low-level laser therapy (LLLT) on the hDPSCs. Conclusion: The findings of this systematic review indicate the useful role of LLLT in cell therapy, proliferation, and differentiation associated with hDPSCs.

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Photobiomodulatory effect delivered by low-level laser on dental pulp stem cell differentiation for osteogenic lineage

Cited by 10 publications

References 37 publications

Osteoblastic differentiation and changes in the redox state in pulp stem cells by laser treatment

Osteoblastic differentiation and changes in the redox state in pulp stem cells by laser treatment

Photo biomodulation of dental derived stem cells to ameliorate regenerative capacity: In vitro study

Effect of Low-Level Laser Therapy on Differentiation and Proliferation of Human Dental Pulp Stem Cells: A Systematic Review

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