Photobioreactor Limnospira indica Growth Model: Application From the MELiSSA Plant Pilot Scale to ISS Flight Experiment

Poughon, Laurent; Creuly, Catherine; Gòdia, Francesc; Leys, Natalie; Dussap, Claude-Gilles

doi:10.3389/fspas.2021.700277

Cited by 8 publications

(5 citation statements)

References 11 publications

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“…As mentioned, literature research revealed that phycocyanin and allophycocyanin constitute between 15% and 20% of the dry weight in Arthrospira when cultured in PBRs (Chaiklahan et al, 2012;Lee et al, 2016;Chaiklahan et al, 2022) which aligns with the values we obtained for the combinations of low light and high temperature (Table 7, 8). Several parameters have an influence on the pigment content.…”

Section: Pigmentssupporting

confidence: 89%

“…The total O 2 production, when normalized per unit leaf area at harvest, was 1. 8 with other investigations on both lettuce and species such as Arabidopsis and kohlrabi, demonstrating a higher chlorophyll concentration in leaves of plants cultivated with a higher proportion of NH 4 + in the fertilizer (Blanke et al, 1996;Qiu et al, 2014;. However, the scientific literature on chlorophyll content as a response to NH 4 + concentration and NH 4 + :NO 3 ratio demonstrate complex relationships and varying results.…”

Section: O 2 Productionmentioning

confidence: 55%

“…4.1.4 Compartment 4: food production 4.1.4.1 Compartment 4a: photoautotrophic cyanobacteria growth The edible microalgae Limnospira platensis and indica (previously Arthrospira platensis and indica) are cyanobacteria that consume nitrate and CO 2 that are produced in other compartments of the loop, and generate oxygen and food for the crew (Clauwaert et al, 2017;Alemany et al, 2019;Sachdeva et al, 2021). Limnospira is grown inside a gas lift photobioreactor with illumination and a gas injection system Poughon et al, 2021). Since a custom biomass composition is used in our model, a new stoichiometric balance had to be calculated (generated biomass is dry mass): .…”

Section: Compartment 3b: Breakdown Of Urea By Ureolytic Heterotrophic...mentioning

confidence: 99%

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Presentations at the 2022 MELiSSA Conference – Current and Future Ways to Closed Life Support Systems

Verseux,

Paul de Vera,

Gennaro Izzo

2024

Frontiers Research Topics

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Frontiers is more than just an open access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers journal seriesThe Frontiers journal series is a multi-tier and interdisciplinary set of openaccess, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers journal series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to qualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation. What are Frontiers Research Topics?Frontiers Research Topics are very popular trademarks of the Frontiers journals series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area.

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Section: Pigmentssupporting

confidence: 89%

Section: O 2 Productionmentioning

confidence: 55%

Section: Compartment 3b: Breakdown Of Urea By Ureolytic Heterotrophic...mentioning

confidence: 99%

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Presentations at the 2022 MELiSSA Conference – Current and Future Ways to Closed Life Support Systems

Verseux,

Paul de Vera,

Gennaro Izzo

2024

Frontiers Research Topics

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“…Photobioreactors can be incorporated into BLSS to increase the production of oxygen by cyanobacteria or algae for enhanced air revitalization. ESA's MELiSSA project is a BLSS concept focused on the regeneration of atmospheric gases and water, waste treatment, and food production for crewed space missions 102,103 . The system comprises the listed compartments, each with a specific organism contributing to the recycling pathway 104 .…”

Section: Atmosphere Revitalizationmentioning

confidence: 99%

Microbial applications for sustainable space exploration beyond low Earth orbit

Koehle,

Brumwell,

Seto

et al. 2023

npj Microgravity

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With the construction of the International Space Station, humans have been continuously living and working in space for 22 years. Microbial studies in space and other extreme environments on Earth have shown the ability for bacteria and fungi to adapt and change compared to “normal” conditions. Some of these changes, like biofilm formation, can impact astronaut health and spacecraft integrity in a negative way, while others, such as a propensity for plastic degradation, can promote self-sufficiency and sustainability in space. With the next era of space exploration upon us, which will see crewed missions to the Moon and Mars in the next 10 years, incorporating microbiology research into planning, decision-making, and mission design will be paramount to ensuring success of these long-duration missions. These can include astronaut microbiome studies to protect against infections, immune system dysfunction and bone deterioration, or biological in situ resource utilization (bISRU) studies that incorporate microbes to act as radiation shields, create electricity and establish robust plant habitats for fresh food and recycling of waste. In this review, information will be presented on the beneficial use of microbes in bioregenerative life support systems, their applicability to bISRU, and their capability to be genetically engineered for biotechnological space applications. In addition, we discuss the negative effect microbes and microbial communities may have on long-duration space travel and provide mitigation strategies to reduce their impact. Utilizing the benefits of microbes, while understanding their limitations, will help us explore deeper into space and develop sustainable human habitats on the Moon, Mars and beyond.

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“…4.1.4 Compartment 4: food production 4.1.4.1 Compartment 4a: photoautotrophic cyanobacteria growth The edible microalgae Limnospira platensis and indica (previously Arthrospira platensis and indica) are cyanobacteria that consume nitrate and CO 2 that are produced in other compartments of the loop, and generate oxygen and food for the crew (Clauwaert et al, 2017;Alemany et al, 2019;Sachdeva et al, 2021). Limnospira is grown inside a gas lift photobioreactor with illumination and a gas injection system (Garcia-Gragera et al, 2021;Poughon et al, 2021). Since a custom biomass composition is used in our model, a new stoichiometric balance had to be calculated (generated biomass is dry mass): (Pannico et al, 2022).…”

Section: Compartment 3b: Breakdown Of Urea By Ureolytic Heterotrophic...mentioning

confidence: 99%

Stoichiometric model of a fully closed bioregenerative life support system for autonomous long-duration space missions

Vermeulen

Papic²,

Nikolič

et al. 2023

Front. Astron. Space Sci.

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Bioregenerative life support systems (BLSS) are vital for long-duration and remote space missions to increase mission sustainability. These systems break down human waste materials into nutrients and CO2 for plants and other edible organisms, which in turn provide food, fresh water, and oxygen for astronauts. The central idea is to create a materially closed loop, which can significantly reduce mission mass and volume by cutting down or even eliminating disposable waste. In most BLSS studies only a fraction of the resources, such as food, are provided by the system itself, with the rest taken on board at departure or provided through resupply missions. However, for autonomous long-duration space missions without any possibility of resupply, a BLSS that generates all resources with minimal or no material loss, is essential. The goal of this study is to develop a stoichiometric model of a conceptually fully closed BLSS that provides all the metabolic needs of the crew and organisms. The MELiSSA concept of the European Space Agency is used as reference system, consisting of five interconnected compartments, each inhabited by different types of organisms. A detailed review of publicly available MELiSSA literature from 1989 to 2022 revealed that no existing stoichiometric model met the study’s requirements. Therefore, a new stoichiometric model was developed to describe the cycling of the elements C, H, O, and N through all five MELiSSA compartments and one auxiliary compartment. A compact set of chemical equations with fixed coefficients was established for this purpose. A spreadsheet model simulates the flow of all relevant compounds for a crew of six. By balancing the dimensions of the different compartments, a high degree of closure is attained at steady state, with 12 out of 14 compounds exhibiting zero loss, and oxygen and CO2 displaying only minor losses between iterations. This is the first stoichiometric model of a MELiSSA-inspired BLSS that describes a continuous provision of 100% of the food and oxygen needs of the crew. The stoichiometry serves as the foundation of an agent-based model of the MELiSSA loop, as part of the Evolving Asteroid Starships (E|A|S) research project.

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Photobioreactor Limnospira indica Growth Model: Application From the MELiSSA Plant Pilot Scale to ISS Flight Experiment

Cited by 8 publications

References 11 publications

Presentations at the 2022 MELiSSA Conference – Current and Future Ways to Closed Life Support Systems

Presentations at the 2022 MELiSSA Conference – Current and Future Ways to Closed Life Support Systems

Microbial applications for sustainable space exploration beyond low Earth orbit

Stoichiometric model of a fully closed bioregenerative life support system for autonomous long-duration space missions

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