Photobiostimulation on chondrocytes proliferation in different concentration of fetal bovine serum under low-level laser irradiation

Zheng, Liqin; Wang, Yuhua; Qiu, Caimin; Chen, Jianlin; Yang, Hongqin; Zhang, Yanding; Xie, Shusen

doi:10.1117/12.2078810

Cited by 1 publication

(2 citation statements)

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“…Rabbit and human chondrocytes in culture treated for 5 days by laser (300 J and 1 W) with varying frequencies (hertz) showed increased cell proliferation that was maintained for five additional days (Torricelli et al, 2001). Chondrocytes treated with LLLI (658, 785, and 830 nm with 10-70 mJ/cm 2 ) in culture with 2 and 5% PBS, but 0 and 10% FBS, responded with significantly promoted proliferation (Zheng et al, 2015). Nevertheless, chondrocytes need to be expanded for several passages to meet the therapeutic requirement (Darling and Athanasiou, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…The use of a red laser (780 nm, 2500 mW) promoted viability and cell metabolism in cultured human chondrocytes (Morrone et al, 2000), and similar laser treatments increased and maintained proliferation of cultured rabbit and human chondrocytes (Torricelli et al, 2001). Low level red light irradiation (658, 785, and 830 nm with 10-70 mJ/cm 2 ) increased proliferation in chondrocytes cultured in medium with 2 and 5% PBS, but not with 0 and 10% FBS (Zheng et al, 2015). However, most experimental evidence using cultured chondrocytes show cell viability results by MTT/MTS assays with few chondrogenic genes examined for expression.…”

Section: Introductionmentioning

confidence: 96%

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Promoted Viability and Differentiated Phenotype of Cultured Chondrocytes With Low Level Laser Irradiation Potentiate Efficacious Cells for Therapeutics

Yang

Liu

et al. 2020

Front. Bioeng. Biotechnol.

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Effective clinical treatments of cartilage lesions in affected joints require large numbers of viable chondrogenic cells generated through in vivo stimulation or ex vivo expansion of chondrocytes isolated from small biopsy specimens. Conventional passaging of chondrocytes in culture provides sufficient cells for treatments but these cells usually lose their differentiated phenotype. This leads to the formation of fibrocartilaginous tissue due to a malfunctioning repair process. Biostimulation of passaging chondrocytes with low level laser irradiation (LLLI) may theoretically produce more functional chondrocytes for cell-based repair of cartilage defects. Molecular and cellular analyses, cytochemistry, cell cultivation, and microscopy showed that LLLI treatments were found to (1) increase chondrocyte viability, (2) promote secretion of matrix proteins, (3) upregulate expression of chondrogenic genes, and (4) downregulate gene expression of cell destructive proteases and genes coding for mediators involved in the extrinsic apoptosis signaling pathway. Furthermore, LLLI attenuated induction of genes associated with cell death and matrix breakdown induced by IL-1β, some of which was seen at the protein level, with verification of effects on gene expression in the C28/I2 human chondrocyte line. LLLI treatments during culture generated larger numbers of viable chondrocytes compared to untreated cultures. Moreover, LLLI-treated chondrocytes in culture also rectified and simultaneously maintained their differentiated phenotype. Cultured chondrocytes treated with LLLI are a promising cell source for repairing cartilage lesions in vivo and restoration of articular function using tissue engineering strategies.

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Section: Discussionmentioning

confidence: 99%