“…Generally, the Fenton-like reaction requires a large amount of H 2 O 2 as a substrate, while the organism does not generate enough H 2 O 2 to supply it, which needs the addition of exogenous H 2 O 2 or the production of H 2 O 2 through relevant reactions. 18,19 In the bacterial infection microenvironment, the levels of antioxidant glutathione (GSH) are abnormally high, and the overexpressed GSH can deplete ROS, leading to a significant decrease in the function of nanozymes. 20 Our team has successfully developed Cu 2 O-demethyleneberberine nanospheres serving as a self-cascading platform, which can exhibit GSH-oxidase (OXD)-like activity to obtain H 2 O 2 through oxidative consumption of GSH for the purpose of continuous supply of H 2 O 2 for the Fenton-like reaction, and then H 2 O 2 is transformed into ˙OH, without the introduction of exogenous H 2 O 2 .…”