Photochemical Cross-Linking for Collagen-Based Scaffolds: A Study on Optical Properties, Mechanical Properties, Stability, and Hematocompatibility

Chan, Barbara Pui; Hui, T.Y.; Chan, Oliver; So, Kwok-Fai; Lü, Wei; Cheung, Kenneth M.C.; Salomatina, Elena; Yaroslavsky, Anna N.

doi:10.1089/ten.2006.12.ft-280

Cited by 11 publications

(30 citation statements)

References 39 publications

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“…To improve the mechanical stability of collagen, crosslinking was used. Previous work has shown that photochemical crosslinking can improve mechanical properties, stability, and haematocompatibility of collagen-based scaffolds [23]. In this study, glutaraldehyde was used as crosslinking agent.…”

Section: Discussionmentioning

confidence: 99%

Construction of Small-Caliber, Polydiaxanone Cyclohexanone Vascular Stents

You

Wang

Duan

et al. 2010

Cell Biochem Biophys

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Objective of this article is to construct and characterize a three-layered small-caliber, artificial vascular stent. The outer layer of the stent consisted of small intestine submucosa (SIS), the middle layer was the polydioxanone (PDS) vascular stents, and the inner layer. The SIS and PDS were attached with 8-0 PDS thread. Crosslinking with a 10% collagen/chondroitin sulfate solution and 0.020% glutaraldehyde secured the structure. The stent was implanted into the muscles on both sides of the canines' spine at 2, 4, 12, and 24 weeks. A MTT assay using L-929 cells measured the cytotoxicity of the implant. Histological and microscopy analyses were employed to examine the degradation characteristics of the PDS stent. Biomechanical properties of the stent were tested and compared to those of normal physiological blood vessels. The PDS stent burst pressure (43.5 +/- 8.3) kPa, rupture intensity (19.1 +/- 1.56) N, strain ratio (42.88 +/- 3.16)%, and radial compliance (5.96 +/- 0.87)%/100 mmHg were similar to that of physiological vessels. The cytotoxicity test showed that the PDS stent complied with specifications for biological materials for medical applications, with a cell toxicity ranging from 0 to 1. After 12 weeks, SIS and collagen sponge were completely replaced by fibrous connective tissue. Although there was some degradation of PDS, inflammatory cell infiltration subsided. After 24 weeks, the scaffold material began to absorb the new fibers and became filled with inflammatory cells and macrophages. This artificial vascular stent met the requirements of transplant experiments and should be further investigated for future clinical applications.

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Section: Discussionmentioning

confidence: 99%

Construction of Small-Caliber, Polydiaxanone Cyclohexanone Vascular Stents

You

Wang

Duan

et al. 2010

Cell Biochem Biophys

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“…The irradiated collagen gel was incubated at room temperature for 0, 3 and 24 h before microstructural analysis. The minimal effective optical penetration depth of laser at 514 nm in collagen gel with different rose Bengal concentrations was $2.5 mm [15], such that laser can penetrate the collagen gel samples in full thickness.…”

Section: Photochemical Crosslinkingmentioning

confidence: 96%

“…Collagen gel was reconstituted as previously described [14,15]. Acid-soluble rat-tail collagen solution type I (Becton Dickinson) was diluted to 4 mg ml À1 using 0.02 N acetic acid.…”

Section: Reconstitution Of Collagen Gelmentioning

confidence: 99%

“…The reconstituted collagen gel was photochemically crosslinked as previously described [14,15]. Collagen gel was equilibrated with a solution of rose Bengal (Sigma) at different concentrations, starting from 98.25 lM with fivefold serial dilution down to 0.1572 lM, for 2 h at room temperature with constant agitation to study the effect of photosensitizer dosage.…”

Section: Photochemical Crosslinkingmentioning

confidence: 99%

“…Recently, the authors have developed a photochemical crosslinking technique, which dramatically enhances the physicochemical properties of collagen-based biomaterials including chemical stability, swelling properties and mechanical properties [14,15]. This technique presents an important biomedical application of photochemistry in addition to photodynamic therapy, in which the chemical reactions are meant to kill cancer cells [16], and photochemical tissue bonding, where tissue surfaces are bonded to assist repair [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

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Effects of photochemical crosslinking on the microstructure of collagen and a feasibility study on controlled protein release

Chan

2008

Acta Biomaterialia

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Photochemical crosslinked electrospun collagen nanofibers: Synthesis, characterization and neural stem cell interactions

Liu

Teng

Chan

et al. 2010

J Biomedical Materials Res

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Currently available crosslinking methods for electrospun collagen nanofibers do not preserve the fibrous architecture over prolonged periods of time. In addition, electrospinning of collagen often involves solvents that lead to extensive protein denaturation. In this study, we demonstrate the advantage of acetic acid over 1,1,1,3,3,3 hexafluoroisopropanol (HFP) in preventing collagen denaturation. A novel photochemical crosslinking method using rose bengal as the photoinitiator is also introduced. Using circular dichorism analyses, we demonstrate the fraction of collagen helical structure to be significantly greater in acetic acid-spun fibers than HFP-spun fibers (28.9 +/- 5.9% vs. 12.5 +/- 2.0%, p < 0.05). By introducing 0.1% (w/v) rose bengal into collagen fibers and subjecting these scaffolds to laser irradiation at a wavelength of 514 nm for 100 sec, biodegradable crosslinked scaffolds were obtained. Scaffold degradation as evaluated by soaking crosslinked collagen scaffolds in PBS at 37 degrees C, indicated a mass loss of 47.7 +/- 7.4% and 68.9 +/- 24.7% at day 7 and day 15, respectively. However, these scaffolds retained fibrous architecture for at least 21 days under physiological conditions. Neural stem cell line, C17.2, cultured on crosslinked collagen scaffolds proliferated after 7 days by forming a confluent layer of cells with extensive cellular projections that were indicative of neurite outgrowth. Taken together, these findings support the potential of acetic acid-electrospun photochemical crosslinked collagen nanofibers for neural tissue engineering.

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Photochemical Cross-Linking for Collagen-Based Scaffolds: A Study on Optical Properties, Mechanical Properties, Stability, and Hematocompatibility

Cited by 11 publications

References 39 publications

Construction of Small-Caliber, Polydiaxanone Cyclohexanone Vascular Stents

Construction of Small-Caliber, Polydiaxanone Cyclohexanone Vascular Stents

Effects of photochemical crosslinking on the microstructure of collagen and a feasibility study on controlled protein release

Photochemical crosslinked electrospun collagen nanofibers: Synthesis, characterization and neural stem cell interactions

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