Photochemical cross-linking of proteins with visible-light-absorbing 1,8-naphthalamides

Judy, Millard M.; Matthews, James L.; Boriak, Richard L.; Burlacu, A.; Lewis, David E.; Utecht, Ronald E.

doi:10.1117/12.147669

Cited by 15 publications

(18 citation statements)

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“…Photons of visible laser irradiation are absorbed by the dye with subsequent release of reactive species that create tissue crosslinks. While the precise mechanism is not fully understood, it has previously been demonstrated that photosensitization can lead to collagen crosslinking [16]. This technique has been successfully applied in a number of tissue injury models.…”

Section: Introductionmentioning

confidence: 97%

Photochemical Tissue Bonding: A Promising Technique for Peripheral Nerve Repair

Johnson

OʼNeill

Motarjem

et al. 2007

Journal of Surgical Research

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Section: Introductionmentioning

confidence: 97%

Photochemical Tissue Bonding: A Promising Technique for Peripheral Nerve Repair

Johnson

OʼNeill

Motarjem

et al. 2007

Journal of Surgical Research

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“…A covalent linkage is ultimately formed between the terminal ends of the intermediate and residues on adjacent biomolecules via a condensation reaction. Research has demonstrated the specificity of this reaction for particular amino acids and biological proteins, as well as Type I collagen in solution, and collagenous biological substrates both in vitro and in vivo . The limited level of cross‐linking lends itself to strengthening the collagen without making it brittle or rigid and is similar to the native collagen cross‐linking configuration .…”

Section: Introductionmentioning

confidence: 99%

“…This research led to the idea that natural vascular scaffolding (NVS), could be used to treat injured collagen and aid in the healing process by restoring its native strength without adding stiffness . This, along with the successful demonstration of in vivo safety and efficacy, prompted research by our group to target use of these compounds for application in vascular systems. The most recent version of this light activated naphthalimide (NVS, Figure ) is designed to localize in the artery …”

Section: Introductionmentioning

confidence: 99%

A novel photochemical cross‐linking technology to improve luminal gain, vessel compliance, and buckling post‐angioplasty in porcine arteries

et al. 2015

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Uninjured arteries have low compliance and low levels of buckling, whereas the BA-injured arteries demonstrated much greater compliance and buckling behavior. Treatment of the injured artery with NVS reduced buckling and demonstrated compliance midway between the two groups while retaining the increased luminal diameter imparted by angioplasty compared to untreated vessels. In summary, limited collagen cross-linking with NVS treatment resulted in lumen retention, as well as improved compliance without the accompanying rigidity and stiffness of conventional stent therapy or current cross-linking materials. This treatment shows great promise for dilation, repair and strengthening of arteries damaged by injury or vascular disease.

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“…Photochemical bonding occurs when a photosensitizer activated by light at a specific wavelength interacts with the substrate directly (type‐I mechanism) or indirectly via singlet oxygen formation (type‐II mechanism) . When collagen is the substrate, radicalization of various amino‐acid groups leads to non‐native crosslinking . Photosensitizers that are activated at higher wavelengths (e.g., 670–690 nm) are advantageous for photochemically bonding cartilage in a clinical setting as higher wavelength light penetrates deeper into the tissue to activate the photosensitizer, potentially achieving a full‐thickness bond with greater strength and improved overall graft integration.…”

mentioning

confidence: 99%

Enhancing integration of articular cartilage grafts via photochemical bonding

Arvayo

Wong

Dragoo

et al. 2018

Journal Orthopaedic Research

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The integration of osteochondral grafts to native articular cartilage is critical as the lack of graft integration may lead to continued tissue degradation, poor load transfer and inadequate nutrient transport. Photochemical bonding promotes graft integration by activating a photosensitizer at the interface via a light source and avoids negative effects associated with other bonding techniques. We hypothesized that the bond strength depends on photosensitizer type and concentration in addition to light exposure. Photochemical bonding was evaluated using methylene blue (MB), a cationic phenothiazine photosensitizer, and two phthalocyanine photosensitizers, Al(III) phthalocyanine chloride tetrasulfonic acid (CASPc) and aluminum phthalocyanine chloride (AlPc). Exposure was altered by varying irradiation time for a fixed irradiance or by varying irradiance with a fixed irradiation time. MB was ineffective at producing bonding at the range of concentrations tested while CASPc produced a peak twofold bond strength increase over controls. AlPc produced substantial bonding at all concentrations with a peak 3.9-fold bond strength increase over controls. Parametric tests revealed that bond strength depended primarily on the total energy delivered to the bonding site rather than the rate of light delivery or light irradiance. Bond strength persisted for 1 week of in-vitro culture, which warrants further exploration for clinical applications. These studies indicate that photochemical bonding is a viable strategy for enhancing articular cartilage graft integration. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:2406-2415, 2018.

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Photochemical cross-linking of proteins with visible-light-absorbing 1,8-naphthalamides

Cited by 15 publications

References 0 publications

Photochemical Tissue Bonding: A Promising Technique for Peripheral Nerve Repair

Photochemical Tissue Bonding: A Promising Technique for Peripheral Nerve Repair

A novel photochemical cross‐linking technology to improve luminal gain, vessel compliance, and buckling post‐angioplasty in porcine arteries

Enhancing integration of articular cartilage grafts via photochemical bonding

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