Photochemical crosslinking of protein and DNA in chromatin

Elsner, Henrik I.; Buchardt, Ole; Møller, Jørgen; Nielsen, Peter E.

doi:10.1016/0003-2697(85)90616-5

Cited by 7 publications

(1 citation statement)

References 28 publications

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“…As an extension of our efforts to design photochemical probes for the study of protein-DNA interactions [17][18][19][20][21][22] we now report that azidoacridine-DNA photoadducts can be cleaved with piperidine and we show that 9-amino-6-azido-2-methoxyacridines can be used as DNA-photocleaving and DNA-photofootprinting reagents.…”

Section: ] Andmentioning

confidence: 85%

Photocleavage of DNA and photofootprinting of E.coli RNA polymerase bound to promoter DNA by azido-9-acridinylamines

Jeppesen¹,

Buchardt

Henriksen

et al. 1988

Nucleic Acids Research

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The long-wavelength ultraviolet (lambda approximately 420 nm) radiation induced reaction between 6-azido-2-methoxy-9-acridinylamines and supercoiled plasmid DNA results in single strand scissions and formation of covalent adducts (ratio approximately 1:10). By treating azidoacridine-photomodified DNA with piperidine at 90 degrees C, additional strand scissions are observed in a complex sequence dependent manner with an overall preference for T greater than or equal to G greater than C much greater than A. The resulting DNA fragments migrate as 5'-phosphates in polyacrylamide gels. Photofootprinting of the binding site of RNA-polymerase on promoter DNA is demonstrated with an azido-9-acridinylamino-octamethylene-9-aminoacridine. Similar experiments using 9-amino-6-azido-2-methoxyacridine indicate that this reagent recognizes changes in the DNA conformation induced by RNA polymerase binding, in relation to open complex formation.

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Section: ] Andmentioning

confidence: 85%

Photocleavage of DNA and photofootprinting of E.coli RNA polymerase bound to promoter DNA by azido-9-acridinylamines

Jeppesen¹,

Buchardt

Henriksen

et al. 1988

Nucleic Acids Research

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Chemical and photochemical probing of DNA complexes

Nielsen

1990

J of Molecular Recognition

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128

107

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An overview of the chemical and photochemical probes which over the past ten years have been used in studies of DNA/ligand complexes and of non-B-form DNA conformations is presented with emphasis on the chemical reactions of the probes with DNA and on their present 'use-profile'. The chemical probes include: dimethyl sulfate, ethyl nitroso urea, diethyl pyrocarbonate, osmium tetroxide, permanganate, aldehydes, methidiumpropyl-EDTA-Fell (MPE), phenanthroline metal complexes and EDTA/FeII. The photochemical probes that have been used include: psoralens, UVB, acridines and uranyl salts. The biological systems analysed by use of these probes are reviewed by tabulation.

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Photofootprinting Analysis of Protein-DNA Interactions

Nielsen

Jeppesen

1989

Photochemical Probes in Biochemistry

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Photochemical crosslinking of protein and DNA in chromatin

Cited by 7 publications

References 28 publications

Photocleavage of DNA and photofootprinting of E.coli RNA polymerase bound to promoter DNA by azido-9-acridinylamines

Photocleavage of DNA and photofootprinting of E.coli RNA polymerase bound to promoter DNA by azido-9-acridinylamines

Chemical and photochemical probing of DNA complexes

Photofootprinting Analysis of Protein-DNA Interactions

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