Photochemotherapy of Skin Diseases

Parrish, John A.; Stern, Robert S.; Pathak, Madhu A.; Fitzpatrick, Thomas B.

doi:10.1007/978-1-4684-8312-3_22

Cited by 93 publications

(49 citation statements)

References 130 publications

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“…The light intensity of near ultraviolet light (315-400 nm) reaching the dermis on a bright sunny day can be calculated to be -20 W/m2 (28). To obtain 20% release of protoporphyrin 40 kJ/m2 is necessary in our in vitro system.…”

Section: Discussionmentioning

confidence: 99%

Light-induced protoporphyrin release from erythrocytes in erythropoietic protoporphyria.

Sandberg¹,

Brun²

1982

J. Clin. Invest.

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A B S T R A C T The photohemolysis of normal erythrocytes incubated with protoporphyrin is reduced in the presence of albumin. When globin is added to normal erythrocytes loaded with protoporphyrin, protoporphyrin is bound to globin. During irradiation protoporphyrin moves from globin to the erythrocyte membrane and photohemolysis is initiated.Erythrocytes in patients with erythropoietic protoporphyria contain large amounts of protoporphyrin bound to hemoglobin. Upon irradiation of these cells in the absence of albumin, 40% of protoporphyrin and 80% of hemoglobin is released after 240 kJ/m2. The released protoporphyrin is hemoglobin bound. In contrast, when albumin is present only 8% of hemoglobin is released whereas protoporphyrin is released to 76%. The released protoporphyrin is albumin bound.A hypothesis for the release of erythrocyte protoporphyrin in erythropoietic protoporphyria without simultaneous hemolysis is proposed: Upon irradiation protoporphyrin photodamages its binding sites on hemoglobin, moves through the plasma membrane, and is bound to albumin in plasma.

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Section: Discussionmentioning

confidence: 99%

Light-induced protoporphyrin release from erythrocytes in erythropoietic protoporphyria.

Sandberg¹,

Brun²

1982

J. Clin. Invest.

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“…Psoralen plus UVA (PUVA) treatment is used in the therapy of psoriasis and other cutaneous disorders [5], although its genotoxic effects have been observed in different organisms [3]. In addition, epidemiological studies have shown that the use of PUVA may increase the incidence of non-melanoma cutaneous cancers [6].…”

Section: Introductionmentioning

confidence: 99%

Genotoxic activity of 4,4?,5?-trimethylazapsoralen on plasmid DNA

Lagatolla

Dolzani

Granzotto

et al. 1998

Teratog. Carcinog. Mutagen.

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“…Psoralens have been used for many years as photoactive probes of nucleic acid structure and function (1), as photoactive drugs against dermatological diseases such as psoriasis (2), and as tools to study the mechanisms of mutagenesis and repair processes (3). Psoralens are bifunctional photoreagents that form covalent bonds with pyrimidine bases of nucleic acids.…”

mentioning

confidence: 99%

Sequence-specific photo-induced cross-linking of the two strands of double-helical DNA by a psoralen covalently linked to a triple helix-forming oligonucleotide.

Takasugi

Guendouz

Chassignol

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

233

165

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On the basis of the structure of DNA-psoralen bis adducts (formed by psoralen with two thymines on opposite strands), a psoralen-oligonucleotide conjugate was designed to photoinduce a cross-link between the two DNA strands at a specific sequence. Psoralen was attached via its C-5 position to a 5'-thiophosphate group of an 11-mer homopyrimidine oligonucleotide. The 11-mer binds to an 11-base-pair homopurinehomopyrimidine sequence of a DNA fragment, where it forms a triple helix. Upon near-UV-irradiation, the two strands of DNA are crosslinked at the TpA step present at the triplex-duplex junction. The reaction is specific for the homopurine'homopyrimidine DNA sequence and requires both oligonucleotide recognition of the DNA major groove and intercalation of psoralen at the triplex-duplex junction. The yield of the photoinduced cross-linking reaction is quite high (>80%). Such psoralen-oligonucleotide conjugates are probes of sequencespecific triple-helix formation and could be used to selectively control gene expression or to induce site-directed mutations.

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Photochemotherapy of Skin Diseases

Cited by 93 publications

References 130 publications

Light-induced protoporphyrin release from erythrocytes in erythropoietic protoporphyria.

Light-induced protoporphyrin release from erythrocytes in erythropoietic protoporphyria.

Genotoxic activity of 4,4?,5?-trimethylazapsoralen on plasmid DNA

Sequence-specific photo-induced cross-linking of the two strands of double-helical DNA by a psoralen covalently linked to a triple helix-forming oligonucleotide.

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