Photocoagulation in rabbits: Optical coherence tomographic lesion classification, wound healing reaction, and retinal temperatures

Koinzer, Stefan; Hesse, Carola; Caliebe, Amke; Saeger, Mark; Baade, Alexander; Schlott, Kerstin; Brinkmann, Ralf; Roider, Johann

doi:10.1002/lsm.22163

Cited by 11 publications

(12 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…OCT classes reflect the intensity of a lesion, or the axial extent, respectively. 24 The correlation of TTC groups and OCT classes was statistically significant (Fisher's exact text, P , 0.001). TTC group 1 achieved predominantly class 1 lesions, but also a significant proportion of class 0 lesions, which were invisible in OCT images (see also Fig.…”

Section: Correlation Of Laser Power and Ophthalmoscopical Diametermentioning

confidence: 87%

“…The temperature range of subvisible lesions is less than 108C, as the RPE viability threshold has been found to be about 538C, 38,41 and visible lesions in rabbits and patients are created at temperatures of little above 608C (OCT class 2 and 3 at 200 ms). 17,24 The temperature increments from one OCT class to the next in 200 ms lesions are in the order of 58C for OCT classes 3 to 5 and about 108C for classes 5 to 6 (in rabbits). The corresponding temperatures for 100 ms lesions have never been determined, but patient data imply that these values should be very similar (difference to 200 ms thresholds is estimated at 18C to 28C).…”

Section: Discussionmentioning

confidence: 99%

“…Edematous halos were excluded from the measurements, because they represent surrounding subretinal edema, but not retinal denaturation. [24][25][26] Every lesion was outlined manually in image editing software (GIMP Ver. 2.8., www.gimp.org), its pixel size measured in ImageJ software (www.rsbweb.nih.gov/ij/) and the pixel and real circle diameters calculated.…”

Section: Color Fundus Imaging and Retinal Lesion Diameter Assessmentmentioning

confidence: 99%

“…24 We chose that method in order to rule out any observer-dependency and bias in the assessment of faint and/or poorly outlined fundus lesions.…”

Section: Color Fundus Imaging and Retinal Lesion Diameter Assessmentmentioning

confidence: 99%

“…24 Characteristics of these intensity classes are reviewed in Figure 4. Lesion intensity will be referred to by the term ''OCT class.…”

Section: Oct Analysismentioning

confidence: 99%

See 4 more Smart Citations

Temperature-Controlled Retinal Photocoagulation Reliably Generates Uniform Subvisible, Mild, or Moderate Lesions

Koinzer

Baade

Schlott

et al. 2015

Trans. Vis. Sci. Tech.

Self Cite

View full text Add to dashboard Cite

Purpose: Conventional retinal photocoagulation produces irregular lesions and does not allow reliable control of ophthalmoscopically invisible lesions. We applied automatically controlled retinal photocoagulation, which allows to apply uniform lesions without titration, and aimed at five different predictable lesion intensities in a study on rabbit eyes.Methods: A conventional 532-nm photocoagulation laser was used in combination with a pulsed probe laser. They facilitated real-time fundus temperature measurements and automatic exposure time control for different predefined time/ temperature dependent characteristics (TTC). We applied 225 control lesions (exposure time 200 ms) and 794 TTC lesions (5 intensities, exposure times 7-800 ms) in six rabbit eyes with variable laser power (20-66.4 mW). Starting after 2 hours, we examined fundus color and optical coherence tomographic (OCT) images over 3 months and classified lesion morphologies according to a seven-stage OCT classifier.Results: Visibility rates in funduscopy (OCT) after 2 hours were 17% (68%) for TTC intensity group 1, 38% (90%) for TTC group 2 and greater than 94% (.98%) for all consecutive groups. TTC groups 1 through 4 correlated to increasing morphological lesion intensities and increasing median funduscopic and OCT diameters. Group 5 lesions were as large as, but more intense than group 4 lesions.Conclusions: Automatic, temperature controlled photocoagulation allows to apply predictable subvisible, mild, or moderate lesions without manual power titration.Translational Relevance: The technique will facilitate standardized, automatically controlled low and early treatment of diabetic retinopathy study (ETDRS) intensity photocoagulation independently of the treating physician, the treated eye and lesion location.

show abstract

Section: Correlation Of Laser Power and Ophthalmoscopical Diametermentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Color Fundus Imaging and Retinal Lesion Diameter Assessmentmentioning

confidence: 99%

“…24 We chose that method in order to rule out any observer-dependency and bias in the assessment of faint and/or poorly outlined fundus lesions.…”

Section: Color Fundus Imaging and Retinal Lesion Diameter Assessmentmentioning

confidence: 99%

“…24 Characteristics of these intensity classes are reviewed in Figure 4. Lesion intensity will be referred to by the term ''OCT class.…”

Section: Oct Analysismentioning

confidence: 99%

See 3 more Smart Citations

Temperature-Controlled Retinal Photocoagulation Reliably Generates Uniform Subvisible, Mild, or Moderate Lesions

Koinzer

Baade

Schlott

et al. 2015

Trans. Vis. Sci. Tech.

Self Cite

View full text Add to dashboard Cite

show abstract

Variability of panretinal photocoagulation lesions across physicians and patients. Quantification of diameter and intensity variation

Saeger

Heckmann

Purtskhvanidze

et al. 2016

Graefes Arch Clin Exp Ophthalmol

View full text Add to dashboard Cite

In this study, the treated area of retina varied by up to a factor of 1.61 = 2.59 for a given spot number. As clinical efficacy depends on the treated area, which is a function of lesion number by area per lesion, our results implicate poor control of the overall treatment effect if treatments are administered according to lesion number or spacing alone. Better ways of laser effect control should be sought.

show abstract

Interpretation of OCT and OCTA images from a histological approach: Clinical and experimental implications

Cuenca

Ortuño‐Lizarán

Sánchez‐Sáez

et al. 2020

Progress in Retinal and Eye Research

View full text Add to dashboard Cite

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

Photocoagulation in rabbits: Optical coherence tomographic lesion classification, wound healing reaction, and retinal temperatures

Cited by 11 publications

References 31 publications

Temperature-Controlled Retinal Photocoagulation Reliably Generates Uniform Subvisible, Mild, or Moderate Lesions

Temperature-Controlled Retinal Photocoagulation Reliably Generates Uniform Subvisible, Mild, or Moderate Lesions

Variability of panretinal photocoagulation lesions across physicians and patients. Quantification of diameter and intensity variation

Interpretation of OCT and OCTA images from a histological approach: Clinical and experimental implications

Contact Info

Product

Resources

About