Photocontrolled DNA nanotubes as stiffness tunable matrices for controlling cellular behavior

Sethi, Soumya; Emura, Tomoko; Hidaka, Kumi; Sugiyama, Hiroshi; Endo, Masayuki

doi:10.1039/d2nr05202d

Cited by 1 publication

(2 citation statements)

References 17 publications

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“…Measured ring diameters decreasing from 3.0 μm to 1.9 μm (Fig. 6b) for increasing temperatures (23,35,40) °C correspond to decreasing effective bending rigidities κ = (2010, 890, 500)k B Tσ according to the simulation data for a fixed attractive strength of 0.3k B T (red circles in Fig. 5c).…”

Section: Experimental Realization Of Dna Ring Contractionmentioning

confidence: 89%

“…Since our micron-sized DNA rings consist of well-established DNA nanotubes, further investigations, e.g. involving mutations like stiffness control 39,40 are facilitated compared to protein-based materials like actin filaments. In the future, these micron-sized DNA rings could be used as tracks for molecular assembly and transport or embedded into adaptive materials.…”

Section: Experimental Realization Of Dna Ring Contractionmentioning

confidence: 99%

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Triggered contraction of self-assembled micron-scale DNA nanotube rings

Illig,

Jahnke,

Weise

et al. 2024

Nat Commun

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Contractile rings are formed from cytoskeletal filaments during cell division. Ring formation is induced by specific crosslinkers, while contraction is typically associated with motor protein activity. Here, we engineer DNA nanotubes and peptide-functionalized starPEG constructs as synthetic crosslinkers to mimic this process. The crosslinker induces bundling of ten to hundred DNA nanotubes into closed micron-scale rings in a one-pot self-assembly process yielding several thousand rings per microliter. Molecular dynamics simulations reproduce the detailed architectural properties of the DNA rings observed in electron microscopy. Theory and simulations predict DNA ring contraction – without motor proteins – providing mechanistic insights into the parameter space relevant for efficient nanotube sliding. In agreement between simulation and experiment, we obtain ring contraction to less than half of the initial ring diameter. DNA-based contractile rings hold promise for an artificial division machinery or contractile muscle-like materials.

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Section: Experimental Realization Of Dna Ring Contractionmentioning

confidence: 89%

Section: Experimental Realization Of Dna Ring Contractionmentioning

confidence: 99%