Photocrosslinkable and elastomeric hydrogels for bone regeneration

Thakur, Teena; Xavier, Janet R.; Cross, Lauren; Jaiswal, Manish K.; Mondragón, Eli; Kaunas, Roland; Gaharwar, Akhilesh K.

doi:10.1002/jbm.a.35621

Cited by 83 publications

(69 citation statements)

References 54 publications

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“…4a), which is consistent with previously reported results. 30 The PAM hydrogel exhibited high porosity characteristic with smaller pore sizes (20 µm) (Fig. 4b),…”

Section: Morphology Of Hydrogelmentioning

confidence: 98%

Biohybrid methacrylated gelatin/polyacrylamide hydrogels for cartilage repair

Han

et al. 2017

J. Mater. Chem. B

143

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Articular cartilage defect repair is challenging for clinics because it is avascular tissue lack of self-regenerative ability. Gelatin-based hydrogels are widely used in the field of tissue engineering because of their good biodegradability, excellent biocompatibility, and cell/tissue affinity. However, gelatin-based hydrogels exhibit poor thermal stability and low mechanical strength, which limits their applications in cartilage repair. In this study, methacrylic anhydride (MA) was employed to modify gelatin to obtain photo-crosslinkable methacrylated gelatin (GelMA). The GelMA-based natural-synthetic polymer biohybrid hydrogel was prepared by co-polymerizing acrylamide (AM) and GelMA under ultraviolet radiation in the presence of a photo-initiator. The GelMA/PAM biohybrid hydrogel simultaneously possessed the advantages of both PAM hydrogels and GelMA hydrogels. The GelMA block provided specific biological functions for cell adhesion and proliferation, while the flexible PAM chains reinforced the brittle gelatin network and sustain load during deformation. Compared with pure PAM hydrogel and GelMA, the GelMA/PAM biohybrid hydrogels showed enhanced compression strength (0.38 MPa) and improved elasticity (storage modulus of 1000 Pa). The GelMA/PAM biohybrid hydrogel showed a favorable degradation rate and sustained protein release. In vitro cell culture showed that the chondrocytes remained viable and proliferated on the biohybrid hydrogel, demonstrating that the biohybrid hydrogels had good cell adhesion and excellent biocompatibility. In a rabbit knee cartilage defect model, we evaluated the cartilage repair ability of the biohybrid hydrogel in vivo. In summary, this study demonstrated that hybridization of synthetic polymers considerably improves the performance and expands the application of the gelatin-based hydrogels. The biohybrid

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“…4a), which is consistent with previously reported results. 30 The PAM hydrogel exhibited high porosity characteristic with smaller pore sizes (20 µm) (Fig. 4b),…”

Section: Morphology Of Hydrogelmentioning

confidence: 98%

Biohybrid methacrylated gelatin/polyacrylamide hydrogels for cartilage repair

Han

et al. 2017

J. Mater. Chem. B

143

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“…Additionally, most of these new developed strategies are evaluated for bone-related applications and very limited studies focus on evaluating these new nanomaterials for other orthopedic tissues including cartilage, tendon, and ligament. [125][126][127] Thus there is a need to investigate these next generation of biomaterials for interface tissue engineering.…”

Section: Emerging Trends and Techniquesmentioning

confidence: 99%

Nanoengineered biomaterials for repair and regeneration of orthopedic tissue interfaces

et al. 2016

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“…2e2). The increased chemical crosslinking resulted in an enhanced physical network 44, 45 . After UV irradiation, a peak centered at 1600 cm −1 appeared (acrylic double bonds, C=C) and at the same time, the peak at 1100 and 1170 cm −1 disappeared (Fig.…”

Section: Resultsmentioning

confidence: 99%

Understanding the impact of crosslinked PCL/PEG/GelMA electrospun nanofibers on bactericidal activity

De-Paula

Ghannadian

Afewerki

et al. 2018

Preprint

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Herein, we report the design of electrospun ultrathin fibers based on polycaprolactone (PCL), polyethylene glycol (PEG), and gelatin methacryloyl (GelMA), and their potential bactericidal activity against three different bacteria Staphylococcus aureus, Pseudomonas aeruginosa, and Methicillin-resistant Staphylococcus aureus (MRSA). We evaluated the morphology, chemical structure and wettability before and after UV photocrosslinking of the produced scaffolds. Results showed that the developed scaffolds presented hydrophilic properties after PEG and GelMA incorporation. Our developed scaffolds were thus able to significantly reduce gram-positive, negative, and MRSA bacteria. Furthermore, we performed a series of study for better mechanistic understanding of the scaffolds bactericidal activity through protein adsorption study and analysis of the reactive oxygen species (ROS) levels. In summary, we have demonstrated the design and generation of electrospun fibers with improved hydrophilicity and efficient bactericidal activity without the association of any antibiotics.

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Photocrosslinkable and elastomeric hydrogels for bone regeneration

Cited by 83 publications

References 54 publications

Biohybrid methacrylated gelatin/polyacrylamide hydrogels for cartilage repair

Biohybrid methacrylated gelatin/polyacrylamide hydrogels for cartilage repair

Nanoengineered biomaterials for repair and regeneration of orthopedic tissue interfaces

Understanding the impact of crosslinked PCL/PEG/GelMA electrospun nanofibers on bactericidal activity

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