Photocurable epsilon-poly-l-lysine for digital light processing 3D printing

Lü, Ying; Xiang, Zuojia; Li, Ning; Huang, Xiaobo; Song, Jianbo

doi:10.1016/j.matlet.2022.132169

Cited by 9 publications

(6 citation statements)

References 7 publications

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“…The amount of methacrylation in EPL-GMA was shown to be 26%. The NMR spectra of SF-GMA and EPL-GMA were comparable to those of previous studies [42,43]. It was confirmed that under the current experimental conditions, GMA modification of SF and EPL could be successfully achieved.…”

Section: Evaluation Of Sf-gma and Epl-gma Polymerssupporting

confidence: 86%

“…Identification of the vinyl peak of GMA in SF-GMA and EPL-GMA confirms the successful methacrylation reaction [38,44]. Peak positions of amide I, II, and III, as well as those of CHOH and CH 2 from SF-GMA and EPL-GMA were comparable with those found in previous studies [38,42,44,59], further confirming successful GMA grafting on SF and EPL.…”

Section: Evaluation Of Sf-gma and Epl-gma Polymerssupporting

confidence: 83%

“…The UV photo-crosslinking of SF-GMA-EPL is predominantly a nucleophilic reaction between NH 2 moieties on the lysine residues of SF or EPL polypeptide chains and activated epoxide groups on GMA grafted on SF and EPL. When exposed to UV light, the photoinitiator LAP initiates the reaction between vinyl double bonds of the methacrylate groups functionalized on SF and EPL in intra-chain and inter-chain manners, resulting in SF-GMA-EPL hydrogel formation [42][43][44]. Along with the vinyl double bond reaction, the entanglement of SF polypeptide chains also occurred, which could enhance β-sheet formation and increase the strength of the hydrogels [38].…”

Section: Preparation Of Sf-gma and Epl-gma Graft Polymers And Sf-gma-...mentioning

confidence: 99%

“…The methacrylation of gelatin, collagen, alginate, and hyaluronic acids [37,[39][40][41] is predominantly achieved through MA. In contrast, SF and EPL methacrylation commonly uses GMA [38,42] that modifies the amino side groups on the polypeptides [38,42].…”

Section: Introductionmentioning

confidence: 99%

“…Although both SF and EPL can be easily modified by GMA [38,[42][43][44], composite GMA-modified SF and EPL hydrogels (SF-GMA-EPL) have yet to be fabricated and studied. It is anticipated that EPL could confer improved antimicrobial properties to the SF based hydrogels.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication and in vitro evaluation of photo cross-linkable silk fibroin–epsilon-poly-L-lysine hydrogel for wound repair

Sundaran,

Kok,

Chang

2023

Biomed. Mater.

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An optimal wound-healing hydrogel requires effective antibacterial properties and a favorable cell adhesion and proliferation environment. Although Bombyx mori silk fibroin (SF) possesses inherent wound-healing properties, it lacks these essential qualities. This study aimed to fabricate a novel photo-polymerizable hydrogel by utilizing SF's wound-healing efficiency and the epsilon-poly-L-lysine (EPL) antimicrobial activity. The SF was modified with three different concentrations of glycidyl methacrylate (GMA) to obtain SF-GMA(L), SF-GMA(M), and SF-GMA(H). A methacrylated EPL (EPL-GMA) was also produced. Then, SF-GMA was mixed with EPL-GMA to produce photo-crosslinkable SF-GMA-EPL hydrogels. The SF-GMA(L)-EPL, SF-GMA(M)-EPL, and SF-GMA(H)-EPL hydrogels, fabricated with 20% EPL-GMA, demonstrated maximum antimicrobial activity and mammalian cell adhesion ability. The hydroxyl radical (•OH) scavenging efficiency of the hydrogels was tested and shown to be between 69% and 74%. These hydrogels also exhibited 60% efficiency in removing bacterial lipopolysaccharides. The water absorption ability of the hydrogels was consistent with the size of their internal pores. The hydrogels exhibited a slow degradation fashion, and their degradation products appeared cytocompatible. Finally, the elastomeric properties of the hydrogels were determined, and a storage modulus (G') of 300–600 Pa was demonstrated. In conclusion, the hydrogels created in this study possess excellent biological and physical properties to support wound healing.

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Section: Evaluation Of Sf-gma and Epl-gma Polymerssupporting

confidence: 86%