The treatment of diabetic skin defects comes with enormous
challenges
in the clinic due to the disordered metabolic microenvironment. In
this study, we therefore designed a novel composite hydrogel (SISAM@HN)
with bioactive factors and tissue adhesive properties for accelerating
chronic diabetic wound healing. Hyaluronic acid (HA) modified by
N
-(2-aminoethyl)-4-(4-(hydroxymethyl)-2-methoxy-5-nitrosophenoxy)
butanamide (NB) held the phototriggering tissue adhesive capacity.
Decellularized small intestinal submucosa (SIS) was degreased and
digested to form the acellular matrix, which facilitated bioactive
factor release. The results of the burst pressure test demonstrated
that the
in situ
formed hydrogel possessed a tissue
adhesive property.
In vitro
experiments, based on
bone marrow stromal cells, revealed that the SIS acellular matrix-containing
hydrogel contributed to promoting cell proliferation.
In vivo
, a diabetic mouse model was created and used to evaluate the tissue
regeneration function of the obtained hydrogel, and our results showed
that the synthesized hydrogel could assist collagen deposition, attenuate
inflammation, and foster vascular growth during the wound healing
process. Overall, the SIS acellular matrix-containing HA hydrogel
was able to adhere to the wound sites, promote cell proliferation,
and facilitate angiogenesis, which would be a promising biomaterial
for wound dressing in clinical therapy of diabetic skin defects.