Microdebris contamination of marine organism has become a major concern. As a detritivore, Tripneustes gratilla also has the opportunity to be contaminated. This study aims to determine microdebris contamination in T. gratilla, including the form and size of the microdebris. This research was conducted at three islands, i.e. Barrang Lompo, Badi, and Kondong Bali. Ten individuals were collected from each island. The digestive tract of spesies was taken, then H2O2 was added to dissolve the organic matter. Flotation was carried out by adding NaCl. Microdebris was observed using stereo microscope, and selected particle then was analysed by FT-IR Microscope. The abundance of microdebris in T. gratilla was ranged from 3.4 to 14.8 particles/individual. Tripneustes gratilla from Badi Island showed the highest levels, contained 14.8 particles/individual. Fiber and fragment were found in all the samples. Fiber was the most common microdebris and consisted of more than half of the total microdebris in each island, except Barrang Lompo. The most common size of microdebris ranged 0.05-1mm, accounted for 36% of the total microdebris. The particle observed by FT-IR Microscope was identified as cotton. Our results suggest that microdebris pollution have contaminated T. gratilla in the Spermonde archipelago.