Photodestruction of human dental plaque bacteria: Enhancement of the photodynamic effect by photomechanical waves in an oral biofilm model

Soukos, Nikolaos S.; Mulholland, Stephen E.; Socransky, S. S.; Doukas, Apostolos G.

doi:10.1002/lsm.10208

Cited by 100 publications

(93 citation statements)

References 58 publications

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“…24,28 It has been suggested that incomplete kill of PDT on biofilms might be because of the failure of PS and light penetration into the inner regions of the biofilm. 39,40 Thus, in comparison with these in vitro studies, the higher PS concentrations and light fluence required for photoinactivation in the present investigation may be explained by the physiological responses of C. albicans to the in vivo environment.…”

Section: Discussionmentioning

confidence: 50%

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Mima

Pavarina

Dovigo

et al. 2010

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

141

130

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Objective. In vivo studies of antimicrobial PDT in animal models of oral candidosis are scarce and the association of porphyrin and LED light has not been evaluated for in vivo photoinactivation of Candida. In this study the effectiveness of photodynamic therapy (PDT) on the inactivation of Candida albicans in vivo was evaluated. Study design. Seventy-one 6-week-old female Swiss mice were immunosuppressed, provided tetracycline to their drinking water, then orally swabbed with a suspension of C. albicans (10 7 CFU/mL). Four days after oral inoculation, PDT was performed on the dorsum of the tongue after topical administration of Photogem at 400, 500, or 1000 mg/L and followed 30 minutes later by illumination with LED light (305 J/cm 2 ) at 455 or 630 nm (n ϭ 5 each). After swabbing to recover yeast from the tongue, the number of surviving yeast cells was determined (CFU/mL) and analyzed by ANOVA and Holm-Sidak tests (P Ͻ .05). Animals were humanely killed, and the tongues surgically removed and processed for histological evaluation of presence of yeast and inflammatory reaction. Results. PDT resulted in a significant reduction in C. albicans recovered from the tongue (P Ͻ .001) when compared with mice from the positive control group. There was no difference between the concentrations of Photogem and LED light wavelengths used. Histological evaluation of the tongue revealed that PDT causes no significant adverse effects to the local mucosa. Conclusion. PDT promoted significant reduction in the viability of C. albicans biofilm without harming the tongue tissue.

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Section: Discussionmentioning

confidence: 50%

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Mima

Pavarina

Dovigo

et al. 2010

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

141

130

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“…26,[49][50][51] In the present study, curcumin was dissolved in BHI broth, because proteins from both saliva and gingival crevicular fluid would also reduce the effect of the photosensitizer in the hypothetical case of its in vivo application. 26 The use of the drug alone (L-D + ) or the light alone (L + D-) did not lead to a significant reduction in the colony-forming unit (CFU) count ( p > 0.05) of any of the microorganisms.…”

Section: Discussionmentioning

confidence: 99%

Photodynamic Effects of Curcumin Against Cariogenic Pathogens

Araújo

Fontana

Bagnato

et al. 2012

Photomedicine and Laser Surgery

101

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Background data: The presence of Streptococcus mutans and Lactobacillus acidophilus in dental structure is an indicator of a cariogenic biofilm. Photodynamic therapy is a technique that involves the activation of photosensitizers by light in the presence of oxygen, resulting in the production of reactive radicals capable of inducing cell death. Reduction of bacteria levels can provide additional means of preventing dental caries. Objective: The present study evaluated the susceptibility of planktonic cultures of S. mutans (ATCC 25175) and L. acidophilus (ATCC-IAL-523) from the Adolfo Lutz Institute (IAL) to photodynamic therapy after sensitization with curcumin and exposure to blue light at 450 nm. Methods: Bacterial suspensions of S. mutans and L. acidophilus isolated (as single species) and combined (multspecies) were prepared and then evaluated. Four different groups were analyzed: L-D-(control group), L-D + (drug group), L + D-(light group), and L + D + (photodynamic therapy group). Two different concentrations of curcumin were tested (0.75 and 1.5 g/L) associated with a 5.7 J/cm 2 light emission diode. Results: Significant decreases ( p < 0.05) in the viability of S. mutans were only observed when the bacterial suspensions were exposed to both curcumin and light. Then, reductions in viability of up to 99.99% were observed when using 1.5 g/L of the photosensitizer. The susceptibility of L. acidophilus was considerably lower (21% and 37.6%) for both curcumin concentrations. Conclusions: Photodynamic therapy was found to be effective in reducing S. mutans and L. acidophilus on planktonic cultures. No significant reduction was found for L-D + , proving the absence of dark toxicity of the drug.

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“…21 The advantage of PDT in reducing periopathogenic microorganisms in vitro is declared by experimental examinations. 22,23 PDT is suggested as a treatment modality that can reduce the demand for flap surgery, and subsequently the chair time and risk of bacteremia. It can also be useful in difficult-to-access areas.…”

mentioning

confidence: 99%

Effect of Nonsurgical Periodontal Treatment Combined With Diode Laser or Photodynamic Therapy on Chronic Periodontitis: A Randomized Controlled Split-Mouth Clinical Trial

et al. 2015

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Photodestruction of human dental plaque bacteria: Enhancement of the photodynamic effect by photomechanical waves in an oral biofilm model

Abstract: PW-assisted photodestruction of dental plaque may be a potentially powerful tool for treatment of chronic destructive periodontal disease.

Cited by 100 publications

References 58 publications

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Photodynamic Effects of Curcumin Against Cariogenic Pathogens

Effect of Nonsurgical Periodontal Treatment Combined With Diode Laser or Photodynamic Therapy on Chronic Periodontitis: A Randomized Controlled Split-Mouth Clinical Trial

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