Photodisruptive laser nucleation of ultrasonic cavitation for biomedical applications

Miller, Douglas L.; Spooner, Greg J. R.; Williams, A.R.

doi:10.1117/1.1380669

Cited by 10 publications

(5 citation statements)

References 25 publications

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“…At each optical breakdown event, a transducer monitors broadband pressure waves generated at the focus of an ultrafast laser source, and simultaneously probes resulting microbubbles through pulse-echo recordings. These microbubbles not only act as site-activated, ultrasonic contrast agents, however, but also as targets for other potential acoustical applications achieving desired biological effects or performing elasticity measurements [18], [19]. To be most useful, however, the LIOB process should operate in controlled modes, creating bubbles with selectable characteristics.…”

Section: Introductionmentioning

confidence: 98%

Acoustic detection of controlled laser-induced microbubble creation in gelatin

Tse

Zohdy

et al. 2005

IEEE Trans. Ultrason., Ferroelect., Freq. Contr.

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A high-frequency (85 MHz) acoustic technique is used to identify system parameters for controlled laser-induced microbubble creation inside tissue-mimicking, gelatin phantoms. Microbubbles are generated at the focus of an ultrafast 793-nm laser source and simultaneously monitored through ultrasonic pulse-echo recordings. Displayed in wavefield form, these recordings illustrate microbubble creation, and integrated backscatter plots provide specifics about microbubble characteristics and dissolution behavior. By varying laser parameters, including pulse fluence (or pulse energy flux, J/cm 2 ), total number of pulses delivered, and the period between pulses, the size, lifetime, and dissolution dynamics of laser-induced microbubbles may be independently controlled. Pulse fluence is the main sizecontrolling parameter, whereas both increases in pulse fluence and pulse number can lengthen microbubble lifetime from tens to hundreds of milliseconds. In short, a microbubble of particular lifetime does not necessarily have to be of a particular size. Microbubble behavior, furthermore, is independent of pulse periods below a fluence-dependent threshold value, but it exhibits stochastic behavior if pulse repetition is too slow. These results demonstrate that laser pulse fluence, number, and period may be varied to deposit energy in a specific temporal manner, creating and stabilizing microbubbles with particular characteristics and, therefore, potential uses in sensitive acoustic detection and manipulation schemes.

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Section: Introductionmentioning

confidence: 98%

Acoustic detection of controlled laser-induced microbubble creation in gelatin

Tse

Zohdy

et al. 2005

IEEE Trans. Ultrason., Ferroelect., Freq. Contr.

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“…Each sample preparation step in a bioanalytical detection system needs to be designed carefully, especially if single cell detection is targeted. It can be expected that technology for the manipulation and analysis of single cells will play an important role in such areas as biomedical research [1,2], drug discovery, diagnosis of disease [3], and medical treatment [4,5].…”

Section: Introductionmentioning

confidence: 99%

Development of a laser-induced cell lysis system

Dhawan

Wise

Baeumner

2002

Analytical and Bioanalytical Chemistry

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A novel cell lysis system was developed that is based on laser-induced disruption of bacterial and yeast cells. It will find application as a rapid, efficient and clean sample preparation step in bioanalytical detection systems. Using E. coli as our model analyte, we optimized cell lysis with respect to optimal laser wavelength, lowest energy input requirements, RNA release from the cells, and potential protein damage. The optimized system was finally applied to the lysis of four additional microorganisms. All experiments were carried out with about 2000 cells per sample or less. Initially, lysis was determined by the detection of cell survival after laser treatment using standard microbiological techniques, (i.e., cells were grown on nutrient agar plates). Then, actual release of mRNA from the cells was proven. Wavelengths investigated ranged from 500 nm to 1550 nm. An average power of 100 mW for the lasers was shown to be sufficient to obtain cell lysis at wavelengths above 1000 nm, with optimal wavelengths between 1250 nm and 1550 nm. Since water absorbs energy at those wavelengths, it is assumed that laser exposure results in an instantaneous increase of the cell temperature, which causes rupture of the cell membrane. Second, damage to protein solutions treated under optimized laser-lysis conditions was also studied. Using a pure solution of horseradish peroxidase as a model protein, no loss in enzyme activity was observed. Thus, it was concluded that damage to intracellular proteins is unlikely. Third, RNA release was tested using an E. coli specific RNA biosensor. Release of RNA was not detected from untreated cells, but laser-treated E. coli cells displayed significant RNA release due to laser-induced cell lysis. Finally, lysis of M. luteus, B. subtilis, B. cereus, and S. cerevisiae were investigated under optimized conditions. In all cases, laser-induced lysis of the cells was confirmed by determination of cell survival. Hence, laser-induced cell lysis is an efficient procedure that can be used for sample preparation, without damage to macromolecules, in bioanalytical detection systems for microorganisms. Miniaturized lasers and miniaturized cell-lysis chambers will create a simple, field-usable cell lysis system and allow the application of laser-induced cell lysis in micro Total Analysis Systems.

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“…73,119 Very localized displacements can be produced and elasticity imaging accomplished in the vicinity of laser and acoustically produced, acoustically driven microbubbles. 120,121 The literature on techniques and applications is too extensive to cover here, but the contributions of the Wisconsin medical physics group are notable. 122,123 Ultrasound contrast agents: Developments and applications of ultrasound contrast agents have been intensely investigated throughout the world, but less so in the USA, where their approved range of applications is extremely limited.…”

Section: Nonlinear Acoustics and Imagingmentioning

confidence: 99%

Anniversary Paper: Evolution of ultrasound physics and the role of medical physicists and the AAPM and its journal in that evolution

Carson

Fenster

2009

Medical Physics

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Ultrasound has been the greatest imaging modality worldwide for many years by equipment purchase value and by number of machines and examinations. It is becoming increasingly the front end imaging modality; serving often as an extension of the physician's fingers. We believe that at the other extreme, high-end systems will continue to compete with all other imaging modalities in imaging departments to be the method of choice for various applications, particularly where safety and cost are paramount. Therapeutic ultrasound, in addition to the physiotherapy practiced for many decades, is just coming into its own as a major tool in the long progression to less invasive interventional treatment. The physics of medical ultrasound has evolved over many fronts throughout its history. For this reason, a topical review, rather than a primarily chronological one is presented. A brief review of medical ultrasound imaging and therapy is presented, with an emphasis on the contributions of medical physicists, the American Association of Physicists in Medicine (AAPM) and its publications, particularly its journal Medical Physics. The AAPM and Medical Physics have contributed substantially to training of physicists and engineers, medical practitioners, technologists, and the public.

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Photodisruptive laser nucleation of ultrasonic cavitation for biomedical applications

Cited by 10 publications

References 25 publications

Acoustic detection of controlled laser-induced microbubble creation in gelatin

Acoustic detection of controlled laser-induced microbubble creation in gelatin

Development of a laser-induced cell lysis system

Anniversary Paper: Evolution of ultrasound physics and the role of medical physicists and the AAPM and its journal in that evolution

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