Photodissociation in a reflectron time-of-flight mass spectrometer:  a novel mass spectrometry/mass spectrometry configuration for high-mass systems

LaiHing, K.; Cheng, Po‐Yuan; Taylor, T. G.; Willey, K. F.; Peschke, Michael; Duncan, M. A.

doi:10.1021/ac00188a031

Cited by 56 publications

(31 citation statements)

References 37 publications

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“…The first TOF is usually the linear type [26] even though the use of reflectron has been reported also [19,27]. The ion gate [28,29] consists of electrodes to deflect away ions which are not selected. Even though substantial progress has been made in the design of the ion gate, selecting a single isotope ion is a difficult task which requires various instrumental arrangements.…”

Section: Tandem Tof Mass Spectrometers: a Brief Reviewmentioning

confidence: 99%

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Tandem time-of-flight mass spectrometer for photodissociation of biopolymer ions generated by matrix-assisted laser desorption ionization (MALDI-TOF-PD-TOF) using a linear-plus-quadratic potential reflectron

Moon

Kim

2004

J. Am. Soc. Mass Spectrom.

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A tandem time-of-flight mass spectrometer for the study of photodissociation of biopolymer ions generated by matrix-assisted laser desorption ionization was designed and constructed. A reflectron with linear and quadratic (LPQ) potential components was used. Characteristics of the LPQ reflectron and its utility as the second stage analyzer of the tandem mass spectrometer were investigated. Performance of the instrument was tested by observing photodissociation of [M ϩ H] ϩ from angiotensin II, a prototype polypeptide. Quality of the photodissociation tandem mass spectrum was almost comparable to that of the post-source decay spectrum. Monoisotopic selection of the parent ion was possible, which was achieved through the ion beam-laser beam synchronization. General theoretical considerations needed for a successful photodissociation of large biopolymer ions are also presented. [3] has revolutionized the application of mass spectrometry for the determination of molecular weights of biopolymers. The natural next step in this field is the tandem mass spectrometry, which detects fragmentation of a mass-selected parent ion. When the internal energy of a polyatomic ion acquired at the time of its formation is sufficient for its dissociation after exiting the source, it may dissociate unimolecularly during its flight to the detector, which is called the metastable ion decomposition (MID) [4]. A more popular way to supply additional energy is to introduce collision gas on the ion flight path such that some of parent ion translational energy is converted to its internal energy. This is called the collision-induced dissociation (CID) or collisionally activated dissociation (CAD) [2]. In the case of the tandem time-of-flight (TOF) mass spectrometry of ions generated by MALDI without the collision gas, the term post source decay (PSD) [5,6] rather than MID has been popular because CID may also contribute to the observed fragment ion signals. When tandem mass spectra generated by PSD are either very weak or do not contain sufficient structural information, CID [7][8][9] may be attempted by introducing collision gas intentionally. Excitation via multiple collisions is thought to be important in the CID tandem mass spectrometry of high mass biopolymers with many degrees of freedom. Recently, dissociation of multiply protonated molecules induced by electron capture, or electron capture dissociation (ECD) [10,11], is attracting a lot of attention as a method to obtain site-specific information.Photodissociation (PD) has been utilized in the field of tandem mass spectrometry mostly as a method to study the structure and dissociation dynamics of small polyatomic ions [12]. Infrared multiphoton dissociation (IRMPD) [13,14] has been used to induce dissociation of biopolymer ions also, even though a large amount of internal energy needed for such a dissociation necessitates absorption of a large number of infrared photons. When electronic transitions of chromophores by ultraviolet radiation are utilized, the number of photons that m...

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Section: Tandem Tof Mass Spectrometers: a Brief Reviewmentioning

confidence: 99%

“…The fact that large biopolymer ions can be photodissociated with pulsed ultraviolet lasers has been demonstrated by several research laboratories [15][16][17][18]. Also, there appeared some reports which presented daughter ion spectra generated by PD [19,20].…”

mentioning

confidence: 99%

Tandem time-of-flight mass spectrometer for photodissociation of biopolymer ions generated by matrix-assisted laser desorption ionization (MALDI-TOF-PD-TOF) using a linear-plus-quadratic potential reflectron

Moon

Kim

2004

J. Am. Soc. Mass Spectrom.

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“…0.4 D active ). Unless the actual beam size is substantially less than indicated by this reasoning, which is difficult to imagine, the resolving powers implicit in Figs 6(e)-(g), 8(c) and 9(c) should be close to the upper limits predicted in each case by Eqn (6). If this is so, the time spread within an ion band entering the ion selector should be close to the zero limit, i.e.…”

Section: Resultsmentioning

confidence: 73%

“…Substituting the values for the resolving powers obtained in the various modes of operation into the left side of Eqn (6) and solving for the effective diameter of the detector yields D effective % 32 mm ($1.3 D active ), 33 mm (1.3 D active ), and 34 mm (1.4 D active ) for single-deflector substance-P, dual-deflector substance-P, and dual-deflector PEG 6000 respectively. These values are remarkably consistent.…”

Section: Resultsmentioning

confidence: 99%

“…LaiHing et al obtained a resolving power of 200 in selecting the metal cluster ion Pb 17 (m/z 3522, 1700 keV) out of a distribution of clusters containing from 2 to 20 atoms. 6 Vlasak et al achieved a resolving power of $170 in isolating diphenylmethane molecular ions (m/z 167, 650 eV), and they estimated that a resolving power greater than 300 should be possible by optimizing their device. 13 These resolving powers are among the highest obtained with single deflectors.…”

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confidence: 99%

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A high resolving power ion selector for post-source decay measurements in a reflecting time-of-flight mass spectrometer

Piyadasa

Håkansson²,

Ariyaratne

et al. 1998

Rapid Commun. Mass Spectrom.

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An electrostatic deflector has been designed and constructed that can be used in a reflecting time-of-flight mass spectrometer for either single-deflector or dual-deflector velocity selection in post-source decay measurements. The deflector consists of an interleaved set of parallel deflection electrodes as in a Loeb/Cravath/Bradbury device, but thin metal ribbon instead of wire is used for the deflection electrodes. The time for reversing the electric field, which depends on various factors such as the electronics for pulsing the voltage and the time constant of a particular electrode geometry, is about 19 ns for the deflectors used in this study. By properly timing the reversal of the electric field, the time-window for ion transmission can be made substantially less than the switching time of each individual deflector. In conjunction with matrix-assisted laser desorption/ionization, the single-deflector's resolving power and transmission are robust with respect to laser fluence, i.e. they remain high even when the fluence is raised well above threshold. By contrast the operational features of the dual-deflector gate offer more versatility in locating and sizing the selection window. Operating the ion selector in a single-deflector mode, we have achieved a resolving power of approximately 710 full width at half maximum (FWHM) for different isotopes of protonated, sodiated, and potassiated substance-P (m/z 1348.6, 1370.6 and 1386.6 respectively; 10.073 keV). Operating it in the dual-deflector mode under two different sets of conditions, we have succeeded in obtaining resolving powers of approximately 1100 (FWHM) for protonated substance-P (m/z 1348.6; 10.8 keV) and approximately 5200 (FWHM) for an isotopomer of PEG 6000 (approximately m/z 6000; 10.04 keV). This accomplishment implies that high-resolution ion selection can be coupled to post-source decay analyses.

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Time-of-flight mass spectrometry: State-of the-art in chemical analysis and molecular science

Weickhardt

Moritz

Grotemeyer

1996

Mass Spectrom. Rev.

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Time‐of‐flight instruments form a well‐established group of mass spectrometers, with its popularity still increasing. This article gives a survey of the technical basics and important instrumental developments in this field. Special notice is taken of factors that limit the mass resolution and to techniques to overcome them. The possibilities to perform tandem MS experiments in flight‐time mass analyzers and their combination with ion‐trapping devices are discussed. Finally, some examples of the modern applications of these instruments in molecular science and instrumental analysis are reviewed. © 1997 John Wiley & Sons, Inc.

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Photodissociation in a reflectron time-of-flight mass spectrometer: a novel mass spectrometry/mass spectrometry configuration for high-mass systems

Cited by 56 publications

References 37 publications

Tandem time-of-flight mass spectrometer for photodissociation of biopolymer ions generated by matrix-assisted laser desorption ionization (MALDI-TOF-PD-TOF) using a linear-plus-quadratic potential reflectron

Tandem time-of-flight mass spectrometer for photodissociation of biopolymer ions generated by matrix-assisted laser desorption ionization (MALDI-TOF-PD-TOF) using a linear-plus-quadratic potential reflectron

A high resolving power ion selector for post-source decay measurements in a reflecting time-of-flight mass spectrometer

Time-of-flight mass spectrometry: State-of the-art in chemical analysis and molecular science

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