Photodissociation of protonated leucine-enkephalin in the VUV range of 8–40 eV

Bari, Sadia; González-Magaña, Olmo; Reitsma, Geert; Werner, James H.; Schippers, S.; Hoekstra, Ronnie; Schlathölter, Thomas

doi:10.1063/1.3515301

Cited by 82 publications

(146 citation statements)

References 45 publications

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“…Experiments using VUV synchrotron radiation confirms that below the ionization threshold mainly a/x-species are formed [15]. The ionic abundances have been reported to increase with the photon energy, which is consistent with the shape of the photoabsorption cross section of the proteins.…”

Section: Results and Applicationssupporting

confidence: 66%

Application of VUV synchrotron radiation to proteomic and analytical mass spectrometry

Giuliani¹,

Milosavljević²,

Canon³

et al. 2013

J. Phys.: Conf. Ser.

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Section: Results and Applicationssupporting

confidence: 66%

Application of VUV synchrotron radiation to proteomic and analytical mass spectrometry

Giuliani¹,

Milosavljević²,

Canon³

et al. 2013

J. Phys.: Conf. Ser.

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“…Different regimes of dissociation processes, below and above ionization energy (IE), have to be considered. For hνGIE, fragmentation is governed by IVR, while for hν9IE, the protonated peptide can be photoionized and a protonated leu-enk dication radical is formed, and the tyrosine side chain fragment in addition to immonium ions become the major fragments for photon energies exceeding 10 eV [44]. Note, however, that for multiply protonated proteins, photoionization is the main fragmentation pattern [31].…”

Section: Comparison With Protonated Peptidesmentioning

confidence: 99%

Formation and Fragmentation of Radical Peptide Anions: Insights from Vacuum Ultra Violet Spectroscopy

Brunet

Antoine

Dugourd

et al. 2011

J. Am. Soc. Mass Spectrom.

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We have studied the photodissociation of gas-phase deprotonated caerulein anions by vacuum ultraviolet (VUV) photons in the 4.5 to 20 eV range, as provided by the DESIRS beamline at the synchrotron radiation facility SOLEIL (France). Caerulein is a sulphated peptide with three aromatic residues and nine amide bonds. Electron loss is found to be the major relaxation channel at every photon energy. However, an increase in the fragmentation efficiency (neutral losses and peptide backbone cleavages) as a function of the energy is also observed. The oxidized ions, generated by electron photodetachment were further isolated and activated by collision (CID) in a MS 3 scheme. The branching ratios of the different fragments observed by CID as a function of the initial VUV photon energy are found to be independent of the initial photon energy. Thus, there is no memory effect of the initial excitation energy on the fragmentation channels of the oxidized species on the time scale of our tandem MS experiment. We also report photofragment yields as a function of photon energy for doubly deprotonated caerulein ions, for both closed-shell ([M -2H] 2-) non-radical ions and open-shell ([M -3H] 2-• ) radical ions. These latter ions are generated by electron photodetachment from [M -3H] 3-precursor ions. The detachment yield increases monotonically with the energy with the appearance of several absorption bands. Spectra for radical and non-radical ions are quite similar in terms of observed bands; however, the VUV fragmentation yield is enhanced by the presence of a radical in caerulein peptides.

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“…They have been in fast development during the last five years, mainly at SOLEIL in France and BESSY II in Germany. Different traps have been used: FT-ICR for the study of singly-charged atomic and small molecular ions as shown in this paper, linear traps for the study of singly-charged atomic ions [32], large molecular [9] and metallic clusters ions [8], RF trap for the study of large molecular ions of biological interest [33][34][35], electron beam ion trap (EBIT) for the study of highly-charged atomic ions [7,36]. To finish, let us note the construction of an electrostatic ring to store the ions at ASTRID II in Denmark, which will combine some of the advantages of both merged beam setup and ion trap.…”

Section: Resultsmentioning

confidence: 99%