Photodynamic action of laser radiation and methylene blue on some opportunistic microorganisms of the oral cavity

Ivanov, Krill I.; Titorenko, Vladimir A.; Shoub, Gennady M.; Лепилин, А. В.; Ovchinnikov, Ilya S.; Mischenko, Oksana S.; Tuchin, Valery V.

doi:10.1117/12.380847

Cited by 3 publications

(4 citation statements)

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“…24 The concentration of 0.01% of MB was selected for being low but still having proven bactericidal effects. 19,25,26 The results showed an increase in K values (dental staining) from T0 to T1 in all groups, demonstrating that even at a very low concentration, the MB was able to penetrate into the dentinal tubules and stain the tooth structures after PDT. Gonçalves also demonstrated dental staining after PDT when using 0.01% MB.…”

Section: Discussionmentioning

confidence: 86%

Effect of Chemical Substances in Removing Methylene Blue After Photodynamic Therapy in Root Canal Treatment

Carvalho

Mello

Albergaria

et al. 2011

Photomedicine and Laser Surgery

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Background and objective: The disadvantage of photodynamic therapy (PDT) is in the photosensitizing agents that may stain the tooth structure. There is no register of PDT studies evaluating protocols to minimize that concern. The present study evaluated the efficiency of chemical adjuncts in methylene blue dye (MB) removal after PDT. Materials and methods: Forty single-rooted teeth, after root canal preparation, were filled with 0.01% MB for 5 min, and irradiated with diode laser 660 nm at 40 mW for 240 sec (total energy 9.6 J). The specimens were divided into four groups (n ¼ 10), according to the chemical adjuncts used for dye removal: (a) 2.5% sodium hypochlorite (NaOCl); (b) 2.5% NaOCl þ Endo-PTC cream; (c) 70% ethyl alcohol and (d) saline (control). The crowns were sectioned and fixed in a device. Photographs were taken before the PDT (T0), immediately after (T1) and upon dye removal (T2). The chromatic alterations were evaluated using Adobe Photoshop and K values were determined in four fixed points of each crown. Results: K values (dental staining) increased in all groups when comparing T0 and T1. The effectiveness of the tested adjuncts was, in decreasing order: G1 (À3.11) > G2 (À2.97) > G3 (À1.28) > G4 (À1.19), not observing significant statistical differences (p < 0.05) between G1 and G2 and also between G3 and G4. Conclusions: Protocols to remove photosensitizing dyes should be applied after PDT in order to minimize dental stain. The protocols tested in this study by using 2.5% NaOCl, associated or not with Endo-PTC cream, were effective in avoiding tooth staining caused by MB during PDT.

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Section: Discussionmentioning

confidence: 86%

Effect of Chemical Substances in Removing Methylene Blue After Photodynamic Therapy in Root Canal Treatment

Carvalho

Mello

Albergaria

et al. 2011

Photomedicine and Laser Surgery

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“…Infiltration of dentinal tubules by MB has been shown by light microscopy, as well as by confocal scanning laser microscopy, 33 and there is strong evidence that light propagates in dentin 34 with dentinal tubules being the main scatterers. 35 Therefore, the generation and diffusion of the reactive-free radicals responsible for the photodynamic effect will be able to fully penetrate dentinal tubules, including the conventionally unreachable areas, and eliminate residual microorganisms.…”

Section: Discussionmentioning

confidence: 97%

Effectiveness of Photodynamic Therapy AgainstEnterococcus faecalis,With and Without the Use of an Intracanal Optical Fiber: AnIn VitroStudy

Nunes

Mello

Franco

et al. 2011

Photomedicine and Laser Surgery

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“…A partir destes processos várias foram as pesquisas demonstrando a inativação de vários tipos de microrganismos utilizando-se os corantes estudados neste estudo (BHATTI et al, 1996(BHATTI et al, , 1997a(BHATTI et al, , 1997b(BHATTI et al, , 1998(BHATTI et al, , 2000PEARSON, 1994;DORTBUDAK et al, 2001aDORTBUDAK et al, , 2001bIVANOV et al, 2000;JACKSON et al, 1996JACKSON et al, , 1999WILSON, 1997;WILSON, 2002;PHOENIX et al, 2003;SEAL et al, 2002;SEGUNDO, 2002;SILBERT et al, 2000;USACHEVA;TEICHERT;BIEL, 2001;HARVEY, 1992;MIA, 1993;MIA, 1994;ZAMPIERI et al, 2003 ZANIN;GONÇALVES, 2002).…”

Section: Discussionunclassified

“…Outra característica a ser considerada quando da escolha de agentes fotossensibilizantes se refere ao desempenho em situações clínicas como, por exemplo, a presença de biofilme dental. Neste aspecto, Wainwright (1998) (BHATTI et al, 1996(BHATTI et al, , 1997a(BHATTI et al, , 1997b(BHATTI et al, , 2001PEARSON, 1994;DORTBUDAK et al, 2001aDORTBUDAK et al, , 2001bIVANOV et al, 2000;JACKSON et al, 1996JACKSON et al, , 1999HOOPER;WILSON, 1997;WILSON, 2002;PHOENIX et al, 2003;SEAL et al, 2002;SILBERT et al, 2000;USACHEVA;TEICHERT;BIEL, 2001;WAINWRIGHT et al, 1999;MIA, 1993;HARVEY., 1992;GONÇALVES, 2002). Outro fator levado em conta foram as diferenças relatadas para microrganismos portadores de membrana externa e também para fungos, onde tais microrganismos apresentavam uma resistência maior a FLL do que outros (USACHEVA; TEICHERT; BIEL, 2001).…”

Section: Discussionunclassified

"Efeito de fotoativadores utilizados na irradiação laser intracanal"

Goncalves¹

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The present study had as objective to evaluate the dental staining after the root canal treatment when it's used the photodynamic therapy, using the dies methylene blue and orto toluidina blue as photosensitizers, through the RGB and K color system from the software Adobe Photoshop. Twenty human single root teeth had its roots canals prepared by the seriated technique using the endodontic instruments plus Endo-PTC and Dakin. Three experimental groups were formed: Group C: dye in its liquid form; Group E: dye associated to the Endo-PTC cream and Group CE: dye in its liquid form with final irrigation of the Dakin plus Endo-PTC for 15 minutes. The color measure was taken before (L0), immediately after (L1) and ten days after the laser irradiation. The statistical analysis performed using the Wilcoxon text proves that the results of the specimens of the E Group on the interactions L1-L2 and L0-L2 didn't have any significant differences. Based on the results obtained it was possible to conclude that: based on the experimental design that performed the traditional endodontic technique, all the activating dyes tested altered the values of K. After carrying out an analysis of the chromatic alteration results according to K values, the best results were observed in specimens of group methylene E and group toluidine E, both of which presented no statistically significant difference for the interaction L0-L1 (p ≤ 5%).

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Photodynamic action of laser radiation and methylene blue on some opportunistic microorganisms of the oral cavity

Cited by 3 publications

References 0 publications

Effect of Chemical Substances in Removing Methylene Blue After Photodynamic Therapy in Root Canal Treatment

Effect of Chemical Substances in Removing Methylene Blue After Photodynamic Therapy in Root Canal Treatment

Effectiveness of Photodynamic Therapy AgainstEnterococcus faecalis,With and Without the Use of an Intracanal Optical Fiber: AnIn VitroStudy

"Efeito de fotoativadores utilizados na irradiação laser intracanal"

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