Photodynamic Diagnoses of Malignant Pleural Diseases Using the Autofluorescence Imaging System

Kitada, Masahiro; Ohsaki, Yoshinobu; Matsuda, Yoshinari; Hayashi, Satoshi; Ishibashi, Keiichiro

doi:10.5761/atcs.oa.14-00162

Cited by 9 publications

(5 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…AF is applied to the diagnosis of cancers in all organs, including laryngeal, bronchial, and lung cancers, and pleural infiltration and peritoneal dissemination of the cancer. 8,9 In particular, the usefulness of thoracoscopy and bronchoscopy for lung cancer 10,11 and endoscopic diagnosis for gastric and colon cancers has been reported. [12][13][14][15][16] Some studies have compared the diagnostic performance of AF observation with endoscopy with white light or NBI observation.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Diagnosis of advanced gastric cancer using image enhancement and autofluorescence imaging systems

Matsui

Inaki

Tsuji

2021

Asian J Endoscop Surgery

View full text Add to dashboard Cite

Introduction: The indication of laparoscopic gastrectomy for advanced cases such as serosal invasion or peritoneal dissemination is still controversial. We report the clinical experiences of laparoscopic diagnosis with image enhancement and autofluorescence (AF) systems for laparoscopic gastric cancer surgery. Methods: The image enhancement system was introduced to 164 patients who underwent laparoscopic gastric cancer surgery as the AF group. The control group was defined as 165 cases who underwent bright light observation without the image enhancement system. The operative findings were recorded during surgery. All image findings were retrospectively compared with the pathological findings. Furthermore, the relationship between the image and pathological findings for peritoneal dissemination and subserosal invasion was evaluated.Results: This study included 329 patients, divided into the control group of 165 patients (50.2%) and the AF group of 164 patients (49.8%). There was no statistically significant difference in patient background between the two groups. In seven cases of peritoneal dissemination, AF was positive, and macroscopic abnormal vascularization was detected. The sensitivity, specificity, and positive and negative predictive values were 100%, 99.4%, 87.5%, and 100%, respectively. In 29 cases of serosal invasion, AF was positive, and macroscopic abnormal vascularization was detected. In the detection accuracy of subserosal invasion, the sensitivity, specificity, and positive and negative predictive values were 75.7%, 97.6%, 90.3%, and 93.2%, respectively. Conclusion:The image enhancement with the AF system is useful for the laparoscopic intraoperative diagnosis of serosal invasion and peritoneal dissemination for advanced gastric cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

“…7 Observations on lung malignant tumors have also been described. 8 However, there have been few reports of using these diagnostic imaging techniques in laparoscopic surgery for gastric cancer. 9 In particular, there are few reports regarding laparoscopic differentiation for serosal invasion or peritoneal dissemination.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of advanced gastric cancer using image enhancement and autofluorescence imaging systems

Matsui

Inaki

Tsuji

2021

Asian J Endoscop Surgery

View full text Add to dashboard Cite

show abstract

“…When 5-aminolevulinic acid (5-ALA) is orally administered, levels of protoporphyrin IX (which emits 635-nm red fluorescence when excited with 405-nm light) increase in cancer tissues [33, 34]. Photofrin® and Lazerphyrin® have been approved and are currently used in PDT in Japan.…”

Section: Discussionmentioning

confidence: 99%

Observation of Zn-photoprotoporphyrin red Autofluorescence in human bronchial cancer using color-fluorescence endoscopy

et al. 2017

Self Cite

View full text Add to dashboard Cite

BackgroundWe observed red autofluorescence emanating from bronchial cancer lesions using a sensitive color-fluorescence endoscopy system. We investigated to clarify the origin of the red autofluorescence.MethodsThe wavelengths of the red autofluorescence emanating from lesions were measured in eight patients using a spectrum analyzer and compared based on pathologic findings. Red autofluorescence at 617.3, 617.4, 619.0, and 617.1 nm was emitted by normal bronchus, inflamed tissue, tissue exhibiting mild dysplasia, and malignant lesions, respectively.Protoporphyrin, uroporphyrin, and coproporphyrin, the major porphyrin derivatives in human blood, were purchased to determine which porphyrin derivative is the source of red fluorescence when acquired de novo. We synthesized photoporphyrin, Zn-protoporphyrin and Zn-photoprotoporphyrin from protoporphyrin.ResultsCoproporphyrin and uroporphyrin emitted only weak fluorescence. Fluorescence was emitted by our synthesized Zn-photoprotoporphyrin at 625.5 nm and by photoprotoporphyrin at 664.0 nm.ConclusionsFrom these results, we conclude that Zn-photoprotoporphyrin was the source of the red autofluorescence observed in bronchial lesions. Zn-protoporphyrin is converted to Zn-photoprotoporphyrin by radiation with excitation light. Our results suggest that red autofluorescence emanating from Zn-photoprotoporphyrin in human tissues could interfere with photodynamic diagnosis using porphyrin derivatives such as Photofrin® and Lazerphyrin® with a sensitive endoscopy system, because color cameras cannot differentiate Zn-photoprotoporphyrin red fluorescence from that of other porphyrin derivatives.

show abstract

“…We have been carrying out studies focused on autofluorescence of normal tissues emitted in response to an excitation wavelength of light. We have also been striving to improve our diagnostic system, by correcting its drawbacks such as unfavorable visualization of lesions and unclear borders between normal tissues and malignant lesions [ 1 ].…”

Section: Discussionmentioning

confidence: 99%

“…Focusing attention on autofluorescence, we endeavored to develop a new method of photodynamic diagnosis (PDD) using an autofluorescence imaging system. However, the initial system had drawbacks such as limitations in the visualization of lesions and unclear borders between normal tissues and malignant lesions [ 1 ]. We thus made efforts to improve the accuracy of this system.…”

Section: Introductionmentioning

confidence: 99%

Photodynamic diagnosis of pleural malignant lesions with a combination of 5-aminolevulinic acid and intrinsic fluorescence observation systems

et al. 2015

Self Cite

View full text Add to dashboard Cite

BackgroundWe have developed a new diagnostic method using the photosensitizer 5-aminolevulinic acid (5ALA) for diagnosing intrathoracic malignant lesions. When ingested exogenously, 5ALA is metabolized to a heme precursor, protoporphyrin IX, which stays in malignant cells and emits red to pink luminescence of about 630 nm.MethodsWe enrolled 40 patients who underwent respiratory surgery and consented to participate in this study. Twenty-eight patients had primary lung cancer, 8 metastatic lung tumors, 2 malignant pleural tumors, and 2 benign tumors. Localization of malignant lesions was attempted by observing such lesions with an autofluorescence imaging system and by comparing the color tone of the autofluorescence between malignant lesions and normal tissues after oral administration of 5ALA. Malignant lesions on the pleural surface emitted pink autofluorescence in contrast to the green autofluorescence of the surrounding normal tissues.ResultsWhen 28 patients with primary lung cancer were examined according to the degree of pleural infiltration (pl), red fluorescence was confirmed in 10 of 10 patients (100%) with p11-p13 and 5 of 18 patients (27.7%) with p10. The latter 5 patients had been diagnosed with PL1 preoperatively or intraoperatively.ConclusionThis system achieved accurate localization of malignant lesions, suggesting that it may also be applicable to photodynamic therapy.

show abstract

Photodynamic Diagnoses of Malignant Pleural Diseases Using the Autofluorescence Imaging System

Cited by 9 publications

References 15 publications

Diagnosis of advanced gastric cancer using image enhancement and autofluorescence imaging systems

Diagnosis of advanced gastric cancer using image enhancement and autofluorescence imaging systems

Observation of Zn-photoprotoporphyrin red Autofluorescence in human bronchial cancer using color-fluorescence endoscopy

Photodynamic diagnosis of pleural malignant lesions with a combination of 5-aminolevulinic acid and intrinsic fluorescence observation systems

Contact Info

Product

Resources

About