Photodynamic effect of protoporphyrin diarginate (PPArg2) on methicillin-resistant Staphylococcus aureus and human dermal fibroblasts.

Grinholc, Mariusz; Kawiak, Anna; Kurlenda, Julianna; Graczyk, Alfreda; Bielawski, Krzysztof Piotr

doi:10.18388/abp.2008_3204

Cited by 21 publications

(21 citation statements)

References 29 publications

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“…A preincubation period of 5-15 min was sufficient for BAM-SiPc to exert its antifungal effect on C. albicans, and further increase in preincubation period did not increase its potency. In this regard, it is similar to the effect of other photosensitisers against C. albicans as reported in previous studies (2-5 min for TBO and 1 min for TriP [4]). 7,16 A total of three different modes of actions of photosensitisers have been reported, viz.…”

Section: Discussionsupporting

confidence: 89%

“…The IC 50 value was 0.013 lmol l )1 (for a cell density of 10 7 cells ml )1 ). Thus, BAM-SiPc seems to be more potent than the cationic porphyrin TriP [4] wherein a concentration as high as 25 lmol l )1 is needed for the destruction of C. albicans. 7 The susceptibility of C. albicans was inversely related to the cell density as higher cell densities resulted in more resistance to killing effect.…”

Section: Discussionmentioning

confidence: 97%

“…3 and methicillin-resistant Staphylococcus aureus. 4 In the wake of such frequent failures of conventional antibiotics, researchers have looked into alternative therapeutic strategies such as PDT for its antimicrobial potency. Traditionally, it is believed that fungi are more resistant to PDT because of their rigid cell wall structure.…”

Section: Introductionmentioning

confidence: 99%

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Photodynamic inactivation ofCandida albicansby BAM-SiPc

So¹,

Tsang²,

et al. 2010

Mycoses

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Photodynamic therapy is a treatment that combines the use of three non-toxic components, viz. photosensitiser, light and oxygen to cause localised oxidative photodamage. In the present study, the antifungal effect of the photosensitiser, BAM-SiPc, an unsymmetrical bisamino phthalocyanine, was investigated. BAM-SiPc was effective in photo-inactivating Candida albicans in a dose-dependent manner. The cell viability as determined by the clonogenic assay was reduced to c. 10% at 0.02 micromol l(-1) BAM-SiPc with a total fluence of 12 J cm(-2) at a cell density of 10(7) cells ml(-1). A short incubation time of 5-15 min was sufficient to allow the photosensitiser to exert its optimal antifungal activity. Microscopical analysis showed that BAM-SiPc was effectively internalised by the fungal cells. Photodynamic treatment led to an increase in the intracellular reactive oxygen species level and disturbed the membrane integrity of the fungal cells.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 97%

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Photodynamic inactivation ofCandida albicansby BAM-SiPc

So¹,

Tsang²,

et al. 2010

Mycoses

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“…The applicator was clamped at a distance of 1-2 mm perpendicularly above each open plate. As each dose was selected, the treatment time was automatically computed by the Solaris device to ensure that the bacteria were irradiated with 0, 1, 3, 5, 7, 9,11,13,15,17,19,25,30,35,40,45, 50, 55, or 60 J/cm 2 .…”

Section: Photo-irradiationmentioning

confidence: 99%

“…Moreover, uncontrolled use of antibiotics continues to worsen the situation [10,11], prompting the exploration of alternative treatment approaches. Other therapies currently under investigation include: the use of antibacterial clay [12], combination of honey and antibiotics [6], hyperbaric oxygen [13,14], photodynamic therapy (PDT) [15], and blue light phototherapy [16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Optimization of the antimicrobial effect of blue light on methicillin-resistantStaphylococcus aureus(MRSA)in vitro

et al. 2015

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Background and Objective In previous studies, we showed that irradiation with 405 nm or 470 nm light suppresses up to 92% methicillin-resistant Staphylococcus aureus (MRSA) growth in vitro and that the remaining bacteria re-colonize. In this study, the aim was to develop a protocol that yields 100% MRSA growth suppression. Materials and Methods We cultured 3 × 106 and 5 × 106 CFU/ml USA300 strain of MRSA and then irradiated each plate with varying fluences of 1–60 J/cm2 of 405 nm or 470 nm light, either once or twice at 6 hours intervals. Next, we plated 7 × 106 CFU/ml and irradiated it with 45, 50, 55, or 60 J/cm2 fluence, once, twice, or thrice at the same 6 hours intervals. In a third experiment, the same culture density was irradiated with 0, 165, 180, 220, or 240 J/cm2, either once, twice, or thrice. Results Irradiation with either wavelength significantly reduced the bacterial colonies regardless of bacterial density (P < 0.05). At 3 × 106 CFU/ml density, nearly 40% and 50% growth of MRSA were suppressed with as little as 3 J/cm2 of 405 nm and 470 nm wavelengths, respectively. Moreover, 100% of the colonies were suppressed with a single exposure to 55 or 60 J/cm2 of 470 nm light or double treatment with 50, 55, or 60 J/cm2 of 405 nm wavelength. At 5 × 106 CFU/ml density, irradiating twice with 50, 55, or 60 J/cm2 of either wavelength suppressed bacterial growth completely, lower fluences did not. The denser 7 × 106 CFU/ml culture required higher doses to achieve 100% suppression, either one shot with 220 J/cm2 of 470 nm light or two shots of the same dose using 405 nm. Conclusion The bactericidal effect of blue light can be optimized to yield 100% bacterial growth suppression, but with relatively high fluences for dense bacterial cultures, such as 7 × 106 CFU/ml.

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Photodynamic therapy with 5‐aminolevulinic acid and diamino acid derivatives of protoporphyrin IX reduces papillomas in mice without eliminating transformation into squamous cell carcinoma of the skin

Kwitniewski

Jankowski

Jaskiewicz

et al. 2009

Intl Journal of Cancer

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Photodynamic therapy (PDT) is used to treat malignant and nonmalignant diseases. It is also used for cosmetological skin treatment. PDT is generally considered to have a low risk of carcinogenicity. However, instances of nonmalignant human tumors turning malignant have been linked to PDT. In this study, we used 5-aminolevulinic (ALA) acid and 3 water soluble photosensitizers-PP(Arg) 2 , PP(Ser) 2 Arg 2 , PP(Ala) 2 Arg 2 , all diamino acid derivatives of protoporphyrin IX-to treat benign papillomas in FVB/N mice induced by 7,12-dimethylbenz(a)anthracene (DMBA)-12-Otetradecanoyl-phorbol-13-acetate (TPA). Of these drugs, ALA and PP(Arg) 2 were found the most efficient. PDT reduced the number of papillomas, but with increasing effectiveness of the drugs, the risk of malignant transformation of the papillomas into squamous cell carcinomas increased. The underlying mechanisms are not clear and further investigations are needed. ' 2009 UICC

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Photodynamic effect of protoporphyrin diarginate (PPArg2) on methicillin-resistant Staphylococcus aureus and human dermal fibroblasts.

Cited by 21 publications

References 29 publications

Photodynamic inactivation ofCandida albicansby BAM-SiPc

Photodynamic inactivation ofCandida albicansby BAM-SiPc

Optimization of the antimicrobial effect of blue light on methicillin-resistantStaphylococcus aureus(MRSA)in vitro

Photodynamic therapy with 5‐aminolevulinic acid and diamino acid derivatives of protoporphyrin IX reduces papillomas in mice without eliminating transformation into squamous cell carcinoma of the skin

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