Photodynamic efficacy of hypericin targeted by two delivery techniques to hepatocellular carcinoma cells

Fadel, Maha; Kassab, Kawser; Youssef, Tareq

doi:10.1007/s10103-010-0787-8

Cited by 15 publications

(7 citation statements)

References 35 publications

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“…The cytocidal effect of PDT on hepatocellular carcinoma (HCC) has been examined in vitro and in vivo in original English articles since 1997 [24][25][26][27][28][29][30][31][32][33]. In the clinically accepted PS worldwide, PDT using 5-ALA showed cytocidal molecular responses in an HCC cell line according to Abo's report [24].…”

Section: Pdt For Primary Liver Malignanciesmentioning

confidence: 99%

Recent Advances in Photodynamic Imaging and Therapy in Hepatobiliary Malignancies: Clinical and Experimental Aspects

et al. 2021

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The therapeutic and diagnostic modalities of light are well known, and derivative photodynamic reactions with photosensitizers (PSs), specific wavelengths of light exposure and the existence of tissue oxygen have been developed since the 20th century. Photodynamic therapy (PDT) is an effective local treatment for cancer-specific laser ablation in malignancies of some organs, including the bile duct. Although curability for extrahepatic cholangiocarcinoma is expected with surgery alone, patients with unresectable or remnant biliary cancer need other effective palliative therapies, including PDT. The effectiveness of PDT for cholangiocarcinoma has been reported experimentally or clinically, but it is not the standard option now due to problems with accompanied photosensitivity, limited access routes of irradiation, tumor hypoxia, etc. Novel derivative treatments such as photoimmunotherapy have not been applied in the field hepatobiliary system. Photodynamic diagnosis (PDD) has been more widely applied in the clinical diagnoses of liver malignancies or liver vascularization. At present, 5-aminolevulinic acid (ALA) and indocyanine green (ICG) dyes are mainly used as PSs in PDD, and ICG has been applied for detecting liver malignancies or vascularization. However, no ideal tools for combining both PDD and PDT for solid tumors, including hepatobiliary malignancies, have been clinically developed. To proceed with experimental and clinical trials, it is necessary to clarify the effective photosensitive drugs that are feasible for photochemical diagnosis and local treatment.

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Section: Pdt For Primary Liver Malignanciesmentioning

confidence: 99%

Recent Advances in Photodynamic Imaging and Therapy in Hepatobiliary Malignancies: Clinical and Experimental Aspects

et al. 2021

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“…as photosensitizer for the photodynamic therapy of cancer by mean its single oxygen production, or it can be used for the photo-diagnosis of early epithelial cancer by taking advantage of its specific accumulation in various types of tumor cells (Kah et al, 2008;Ritz et al, 2008). Among different targets, tumor melanocytes (Hadjur et al, 1996), glioma cells (Huntosova et al, 2012), isolated crayfish neuron (Uzdensky et al, 2003) and hepatocellular carcinoma cells (Fadel et al, 2010) were quoted and reported as cell models for the study of the photodynamic efficacy of hypericin after photoirradiation.…”

Section: Features Of Hypericinmentioning

confidence: 99%

Novel Neuroprotective Formulations Based on St. John’s Wort Extract

Vazzana¹,

Macedo²,

Santini³

et al. 2014

JFR

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St. John's Wort (SJW) has been intensively studied in the last years with respect to its pharmacological properties and to understand the mechanism of action of its bioactive compounds. In fact, it is currently used for the treatment of several disorders. Nevertheless, only recently nanotechnology has been applied for the delivery of SJW extract in vivo, to enhance its neuroprotective properties. In the present review, the advantages, the chemical characterization and the special biological features of SJW extract are discussed, underlining the potential use of nanotechnology in the development of drug carrier systems based on lipid nanoparticles. A special focus is given to solid lipid nanoparticles (SLN) and to nanostructured lipid carriers (NLC) given their versatility for a wide range of bioactive compounds.

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“…43−46 Moreover, encapsulation increased the photostability of hypericin, and in some cases, this encapsulation led to a decrease in its phototoxicity. 43,47 For these reasons, we investigated the phototoxicity and PDT efficacy of the newly constructed nanoparticles in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

Alkyl Chain Length in Poly(2-oxazoline)-Based Amphiphilic Gradient Copolymers Regulates the Delivery of Hydrophobic Molecules: A Case of the Biodistribution and the Photodynamic Activity of the Photosensitizer Hypericin

et al. 2021

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Self-assembled nanostructures of amphiphilic gradient copoly(2-oxazoline)s have recently attracted attention as promising delivery systems for the effective delivery of hydrophobic anticancer drugs. In this study, we have investigated the effects of increasing hydrophobic side chain length on the selfassembly of gradient copolymers composed of 2-ethyl-2-oxazoline as the hydrophilic comonomer and various 2-(4-alkyloxyphenyl)-2oxazolines as hydrophobic comonomers. We show that the size of the formed polymeric nanoparticles depends on the structure of the copolymers. Moreover, the stability and properties of the polymeric assembly can be affected by the loading of hypericin, a promising compound for photodiagnostics and photodynamic therapy (PDT). We have found the limitation that allows rapid or late release of hypericin from polymeric nanoparticles. The nanoparticles entering the cells by endocytosis decreased the hypericininduced PDT, and the contribution of the passive process (diffusion) increased the probability of a stronger photoeffect. A study of fluorescence pharmacokinetics and biodistribution revealed differences in the release of hypericin from nanoparticles toward the quail chorioallantoic membrane, a preclinical model for in vivo studies, depending on the composition of polymeric nanoparticles. Photodamage induced by PDT in vivo well correlated with the in vitro results. All formulations studied succeeded in targeting hypericin at cancer cells. In conclusion, we demonstrated the promising potential of poly(2-oxazoline)-based gradient copolymers for effective drug delivery and sequential drug release needed for successful photodiagnostics and PDT in cancer therapy.

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Photodynamic efficacy of hypericin targeted by two delivery techniques to hepatocellular carcinoma cells

Cited by 15 publications

References 35 publications

Recent Advances in Photodynamic Imaging and Therapy in Hepatobiliary Malignancies: Clinical and Experimental Aspects

Recent Advances in Photodynamic Imaging and Therapy in Hepatobiliary Malignancies: Clinical and Experimental Aspects

Novel Neuroprotective Formulations Based on St. John’s Wort Extract

Alkyl Chain Length in Poly(2-oxazoline)-Based Amphiphilic Gradient Copolymers Regulates the Delivery of Hydrophobic Molecules: A Case of the Biodistribution and the Photodynamic Activity of the Photosensitizer Hypericin

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