Photodynamic inactivation of Bovine herpesvirus type 1 (BoHV-1) by porphyrins

Teles, Amanda Vargas; Oliveira, Taise M. A.; Bezerra, Fábio Castro; Alonso, Laís; Alonso, Antônio; Borissevitch, Iouri; Gonçalves, Pablo José; Souza, Guilherme Rocha Lino de

doi:10.1099/jgv.0.001121

Cited by 26 publications

(5 citation statements)

References 29 publications

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“…Referring to the concentration range of porphyrin-type photosensitizer molecules used in the literature on photodynamic inactivation of viruses in the range of 0.1–25 μM, − we chose a moderate concentration of PpIX: 7.8 μM (0.2 mg/mL SiO 2 –PpIX) for virus inactivation research. We studied the photodynamic inactivation against VSV and HSV-1 (Figure A,C, respectively) with 0.2 mg/mL SiO 2 –PpIX.…”

Section: Resultsmentioning

confidence: 99%

Efficient Inactivation of Enveloped Viruses Using a Nanoparticle-Based Photodynamic Method

Zhang,

Kuang,

Liu

et al. 2023

ACS Appl. Nano Mater.

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Enveloped viruses pose a critical health threat to human beings. Photodynamic inactivation shows promise but requires a relatively long time of irradiation or a high power intensity. Meanwhile, the unclear role of reactive oxygen species (ROS) in inactivation hampers the development of effective antivirus equipment. Here, we present that protoporphyrin IX-loaded silica nanoparticles show high efficiency in inactivating enveloped viruses. Representative enveloped viruses, including herpes simplex virus 1, vesicular stomatitis virus, and pseudoviruses SARS-CoV-2, were almost fully inactivated under <16 min of irradiation of a halogen tungsten lamp. By a quantitative polymerase chain reaction technique, we found that the viruses lost their ability to bind cells after the photoinactivation. The mechanistic study further showed that the nanoparticle-based photodynamic effect disrupted the receptor-binding proteins on the envelope rather than destroying the whole virus structure by ROS. Our results suggest a highly efficient approach to disinfecting enveloped viruses and may shed light on designing photodynamic materials for breaking spreading chains in epidemics.

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Section: Resultsmentioning

confidence: 99%

Efficient Inactivation of Enveloped Viruses Using a Nanoparticle-Based Photodynamic Method

Zhang,

Kuang,

Liu

et al. 2023

ACS Appl. Nano Mater.

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“…Herpes virus infection has serious complications especially in immunocompromised patients, pregnant women and infants [117]. In the following work the authors studied photodynamic activity of cationic TMPyP and anionic TPPS 4 , as well as their zinc complexes (ZnTMPyP and ZnTPPS 4 ) in inactivating bovine herpes virus type 1 (BoHV-1) [118] In dark toxicity studies, none of the samples affected virus viability. However, when BoHV-1 samples were irradiated in the presence of porphyrins, the effectiveness of the studied photosensitizers decreased in the following order: ZnTMPyP > TMPyP > ZnTPPS4 > TPPS 4 .…”

Section: •−mentioning

confidence: 99%

Cationic Porphyrins as Antimicrobial and Antiviral Agents in Photodynamic Therapy

Savelyeva,

Zhdanova,

Gradova

et al. 2023

CIMB

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Antimicrobial photodynamic therapy (APDT) has received a great deal of attention due to its unique ability to kill all currently known classes of microorganisms. To date, infectious diseases caused by bacteria and viruses are one of the main sources of high mortality, mass epidemics and global pandemics among humans. Every year, the emergence of three to four previously unknown species of viruses dangerous to humans is recorded, totaling more than 2/3 of all newly discovered human pathogens. The emergence of bacteria with multidrug resistance leads to the rapid obsolescence of antibiotics and the need to create new types of antibiotics. From this point of view, photodynamic inactivation of viruses and bacteria is of particular interest. This review summarizes the most relevant mechanisms of antiviral and antibacterial action of APDT, molecular targets and correlation between the structure of cationic porphyrins and their photodynamic activity.

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“…215 Similarly, a comparative study of cationic and anionic PSs with bovine herpes virus (BoHV) type-1 indicated a higher activity for zinc(II) complexes. 217 In a concurrent approach, 5,10,15,20-tetrakis(4sulfonatophenyl)porphyrin has been loaded onto fullerene. This nanoparticle PS was effective in the PDI of HSV-1, the PS loaded on fullerene being more efficient than the free PS.…”

Section: Figure 10mentioning

confidence: 99%