Photodynamic inactivation of Candida albicans biofilm: Influence of the radiant energy and photosensitizer charge

Sousa, Aline Silva; Prates, Renato Araújo; Santi, Maria Eugenia Simões Onofre de; Lopes, Rubia Garcia; Bussadori, Sandra Kalil; Ferreira, Luis Rodolfo; Deana, Alessandro Melo

doi:10.1016/j.pdpdt.2016.03.004

Cited by 24 publications

(8 citation statements)

References 29 publications

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“…However, their application in preventing and treating infections in humans, animals, and plants has led to the emergence and spread of antimicrobial resistance (CDC, 2015). As well as the development of drug resistance, conventional drugs also possess undesired adverse effects (Sousa et al, 2016), prompting researchers to look for alternative antimicrobial agents (with little or no adverse effects) to evade the drawbacks that accompany drug resistance.…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of omega-3 polyunsaturated fatty acids against microbial pathogens

Chanda

Joseph

Guo

et al. 2018

J. Zhejiang Univ. Sci. B

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Microorganisms provide both beneficial and harmful effects to human beings. Beneficial effects come from the symbiotic relationship that exists between humans and microbiota, but then several human illnesses have turned some friendly microbes into opportunistic pathogens, causing several microbial-related diseases. Various efforts have been made to create and utilize antimicrobial agents in the treatment and prevention of these infections, but such efforts have been hampered by the emergence of antimicrobial resistance. Despite extensive studies on drug discovery to alleviate this problem, issues with the toxicity and tolerance of certain compounds and continuous microbial evolution have forced researchers to focus on screening various phytochemical dietary compounds for antimicrobial activity. Linolenic acid and its derivatives (eicosapentaenoic acid and docosahexaenoic acid) are omega-3 fatty acids that have been studied due to their role in human health, being important for the brain, the eye, the cardiovascular system, and general human growth. However, their utilization as antimicrobial agents has not been widely appreciated, perhaps due to a lack of understanding of antimicrobial mechanisms, toxicity, and route of administration. Therefore, this review focuses on the efficacy, mechanism, and toxicity of omega-3 fatty acids as alternative therapeutic agents for treating and preventing diseases associated with pathogenic microorganisms.

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Section: Introductionmentioning

confidence: 99%

Effectiveness of omega-3 polyunsaturated fatty acids against microbial pathogens

Chanda

Joseph

Guo

et al. 2018

J. Zhejiang Univ. Sci. B

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“…Comparing with the results in planktonic state (Figure 1), it is apparent that photoinactivation is not as efficient and no disinfecting effect (>99.999% kill) could be achieved for any isolate despite increasing the concentration of MB by 2.5-fold. It is well known that photoinactivation of cells living in biofilms require higher concentration of MB compared to their planktonic counterparts [39,40], owing mainly to the mechanical barrier to MB diffusion posed by the extracellular matrix and to alterations in gene expression. As in planktonic cells, statistically significant differences were detected among the strains: Firstly, none of the multidrug-resistant strains appear to be more tolerant to aPDT than the reference ATCC 25922.…”

Section: Discussionmentioning

confidence: 99%

Effective Photodynamic Inactivation of 26 Escherichia coli Strains with Different Antibiotic Susceptibility Profiles: A Planktonic and Biofilm Study

Gulías

McKenzie

Bayó³

et al. 2020

Antibiotics

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The emergence of multidrug-resistant bacteria is a growing problem and alternative therapies are being sought to effectively address this issue. The aim of this study is to assess a range of Escherichia coli strains’ susceptibility to Methylene Blue-mediated antimicrobial photodynamic therapy and determine if this is affected by their antibiotic-resistance profile. Two reference and twenty-four uropathogenic clinical E. coli strains were used in this study. All were tested in vitro for antimicrobial susceptibility against sixteen antibiotics. Strains underwent photodynamic treatments using the photosensitizer Methylene Blue with red light and tested in both planktonic and biofilm state. It was found that reference strain ATCC 25922 was susceptible to all tested antibiotics whereas reference strain ATCC 35218 showed resistance only to Ampicillin. With the exception of strains number 16 and 22, all of the isolated strains were multidrug-resistant according to the criteria established by the European Centre for Disease Prevention and Control and the Centre for Disease Control and Prevention, where acquired non-susceptibility to at least one agent in three or more antimicrobial categories is outlined. Photodynamic therapy induced more than 3 log10 colony-forming units’ reduction to all strains in planktonic state. Whereas when tested in biofilm state, two and a half times the original dose of methylene blue was necessary to cause a 3 log10 antimicrobial effect. There were statistically significant differences in susceptibility among the strains tested in both the planktonic and biofilm experiments. Nevertheless, antimicrobial photodynamic therapy could inactivate all multidrug-resistant strains in the planktonic and biofilm state.

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“…Yeasts are less sensitive to PS agents compared to bacteria because of their size and the presence of a thick cell wall, so only a few PSs and light sources can be used in order to successfully eliminate them ( Sousa et al, 2016 ; Wiench et al, 2019 ). The thin channels in the cell wall prevent the PS from passing through the wall; therefore, cationic PS and more extended contacts with the wall than the gram-negative bacteria are needed to ensure the yeast’s death.…”

Section: Applications Of Apdt In the Treatment Of Oral Fungal Infectionsmentioning

confidence: 99%

Clinical applications of antimicrobial photodynamic therapy in dentistry

et al. 2023

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Given the emergence of resistant bacterial strains and novel microorganisms that globally threaten human life, moving toward new treatment modalities for microbial infections has become a priority more than ever. Antimicrobial photodynamic therapy (aPDT) has been introduced as a promising and non-invasive local and adjuvant treatment in several oral infectious diseases. Its efficacy for elimination of bacterial, fungal, and viral infections and key pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Candida albicans, and Enterococcus faecalis have been investigated by many invitro and clinical studies. Researchers have also investigated methods of increasing the efficacy of such treatment modalities by amazing developments in the production of natural, nano based, and targeted photosensitizers. As clinical studies have an important role in paving the way towards evidence-based applications in oral infection treatment by this method, the current review aimed to provide an overall view of potential clinical applications in this field and summarize the data of available randomized controlled clinical studies conducted on the applications of aPDT in dentistry and investigate its future horizons in the dental practice. Four databases including PubMed (Medline), Web of Science, Scopus and Embase were searched up to September 2022 to retrieve related clinical studies. There are several clinical studies reporting aPDT as an effective adjunctive treatment modality capable of reducing pathogenic bacterial loads in periodontal and peri-implant, and persistent endodontic infections. Clinical evidence also reveals a therapeutic potential for aPDT in prevention and reduction of cariogenic organisms and treatment of infections with fungal or viral origins, however, the number of randomized clinical studies in these groups are much less. Altogether, various photosensitizers have been used and it is still not possible to recommend specific irradiation parameters due to heterogenicity among studies. Reaching effective clinical protocols and parameters of this treatment is difficult and requires further high quality randomized controlled trials focusing on specific PS and irradiation parameters that have shown to have clinical efficacy and are able to reduce pathogenic bacterial loads with sufficient follow-up periods.

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Photodynamic inactivation of Candida albicans biofilm: Influence of the radiant energy and photosensitizer charge

Cited by 24 publications

References 29 publications

Effectiveness of omega-3 polyunsaturated fatty acids against microbial pathogens

Effectiveness of omega-3 polyunsaturated fatty acids against microbial pathogens

Effective Photodynamic Inactivation of 26 Escherichia coli Strains with Different Antibiotic Susceptibility Profiles: A Planktonic and Biofilm Study

Clinical applications of antimicrobial photodynamic therapy in dentistry

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