Photodynamic inactivation of Escherichia coli bacteria by cationic photosensitizers

Меерович, Г. А.; Akhlyustina, E. V.; Denisov, D S; Kholina, Ekaterina G.; Макарова, Е. А.; Luk’yanets, E. A.; Loschenov, Victor B.; Strakhovskaya, M. G.

doi:10.1088/1612-202x/ac2cd1

Cited by 7 publications

(2 citation statements)

References 23 publications

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“…It should be noted that many tetrapyrroles that are promising for APDT, including derivatives of phthalocyanines [22] and synthetic bacteriochlorin [23], have not a planar, but rather a three-dimensional spatial structure, and are less susceptible to aggregation in an aqueous medium and, presumably, when bound to gram-negative bacteria.…”

Section: Discussionmentioning

confidence: 99%

Оn the aggregation of polycationic photosensitizer upon binding to Gram-negative bacteria

Romanishkin,

Akhlyustina,

Meerovich

et al. 2024

Methods Appl. Fluoresc.

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Polycationic photosensitizers (PS) are not susceptible to aggregation in solutions, but their high local concentrations in Gram-negative bacteria can be sufficient for aggregation and reduced effectiveness of antibacterial photodynamic treatment. By measuring fluorescence spectra and kinetics we were able to evaluate the degree of aggregation of polycationic PS ZnPcChol8 in Gram‑negative bacteria E. coli K12 TG1. Binding of ZnPcChol8 to E. coli K12 TG1 leads to an appearance of groups of molecules with shorter PS fluorescence lifetime, a decrease in fluorescence intensity and a shift in the fluorescence spectral maximum. However, we evaluated that about 88% of the fluorescing PS molecules in the bacteria were in an unaggregated state, which indicates only a small reduction in the generation of reactive oxygen species.

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Section: Discussionmentioning

confidence: 99%

Оn the aggregation of polycationic photosensitizer upon binding to Gram-negative bacteria

Romanishkin,

Akhlyustina,

Meerovich

et al. 2024

Methods Appl. Fluoresc.

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“…In our previous studies, octakis(cholinyl)zinc phthalocyanine (ZnPcChol 8+ ) demonstrated electrostatic binding to bacterial cells [26,27] and enveloped viruses [28,29], and readily sensitized targeted pathogens in suspensions when activated with far red light [30][31][32][33]. Given the large overall positive charge of ZnPcChol 8+ , it can be expected that the molecules of this PS will effectively compete with the cations contained in the droplets for binding sites on SARS-CoV-2 virions and potentiate photodynamic inactivation.…”

Section: Introductionmentioning

confidence: 99%

Photodynamic inactivation of SARS-CoV-2 on inanimate surfaces

Kurskaya¹,

Sharshov²,

Соломатина³

et al. 2022

Laser Phys. Lett.

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Since coronaviruses can remain infectious on different inanimate surfaces for several hours or even days, the possibility of indirect fomite transmission through infected objects and surfaces cannot be ruled out. We describe a method for the photodynamic disinfection of inanimate surfaces infected with severe acute respiratory syndrome coronavirus 2, Omicron variant strain. Application of only 5 µM photosensitizer octakis(cholinyl)zinc phthalocyanine followed immediately by 7 min irradiation with light emitting diode (LED) light 692 nm (12.5 mW cm−2) results in complete inactivation of the virus on polystyrene and glass surfaces, while 10 min irradiation lead to complete eradication of the virus also on Al-foil and medical mask fabric. A photodynamic technique is being considered to combat the spread of coronaviruses.

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Photoinactivation of Escherichia coli and Staphylococcus aureus using Porphyrin-CuInS2/ZnS quantum dot conjugates immobilized on Mesoporous Silica

Ndlovu,

Chokoe,

Masebe

et al. 2024

Chem. Pap.

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Bacterial pathogens remain major contributors to illnesses as they have developed several resistance mechanisms against standard treatments. Innovative porphyrin-quantum dots conjugated materials have great potential in addressing the limitations in the current disinfection methods. The antimicrobial activity of metal-free and In(III) derivative of 4-(15-(4-boronophenyl)-10,20-diphenylporphyrin-5-yl)benzoic acid conjugated to CuInS2/ZnS quantum dots is investigated in this study at laboratory-scale experiments under controllable conditions. The conjugate was also immobilized on mesoporous silica for recovery and reusability purposes. Findings of the study were driven by antimicrobial photodynamic inactivation (aPDI) in the presence of a porphyrin and quantum dots. POR(In)-CIS/ZnS QDs-Silica was the best performing conjugate with a singlet quantum yield (ΦΔ) of 0.72 and a log reduction of 9.38 and 9.76 against Escherichia coli and S. aureus, respectively.

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Photodynamic inactivation of Escherichia coli bacteria by cationic photosensitizers

Cited by 7 publications

References 23 publications

Оn the aggregation of polycationic photosensitizer upon binding to Gram-negative bacteria

Оn the aggregation of polycationic photosensitizer upon binding to Gram-negative bacteria

Photodynamic inactivation of SARS-CoV-2 on inanimate surfaces

Photoinactivation of Escherichia coli and Staphylococcus aureus using Porphyrin-CuInS2/ZnS quantum dot conjugates immobilized on Mesoporous Silica

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