Photodynamic suppression of <i>Enterococcus faecalis</i> using the photosensitizer mTHPC

Kranz, Stefan; Guellmar, André; Völpel, Andrea; Gitter, Burkhard; Albrecht, Volker; Sigusch, Bernd W.

doi:10.1002/lsm.21046

Cited by 44 publications

(49 citation statements)

References 64 publications

(75 reference statements)

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“…In vitro, ex vivo, and in vivo studies using aPDT have shown that this approach has the potential to maximize root canal disinfection (18)(19)(20)(28)(29)(30)(31)(32). However, to the best of the authors' knowledge, there is so far no study evaluating the response of apical and periapical tissues to the use of aPDT in the endodontic treatment of teeth with apical periodontitis (ie, with the presence of tissue infection at the beginning of the treatment).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Photodynamic Therapy for the Treatment of Teeth with Apical Periodontitis: A Histopathological Evaluation

Novaes

Oliveira

Filho

et al. 2012

Journal of Endodontics

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Photodynamic Therapy for the Treatment of Teeth with Apical Periodontitis: A Histopathological Evaluation

Novaes

Oliveira

Filho

et al. 2012

Journal of Endodontics

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“…34 Tests with liposomal mTHPC formulation demonstrated the complete inactivation of E. faecalis after a total energy dose of 100 J cm −2 , but at a higher concentration of PS (50 μM). 28 The natural chlorin Ce 6 and its conjugates with poly-L-lysine ( pL) or polyethyleneimine are among the chlorin derivatives with high antimicrobial activity. An ultra-pure Ce 6 at the concentration of 20 μM showed strong inactivation of P. aeruginosa and E. coli, using a red laser diode (λ = 664 nm, 588 mW cm −2 ) as a light source.…”

Section: Discussionmentioning

confidence: 99%

Comparative photodynamic inactivation of antibiotic resistant bacteria by first and second generation cationic photosensitizers

Costa

Gomes

Faustino

et al. 2012

Photochem Photobiol Sci

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Photodynamic inactivation (PDI) is an efficient approach against a wide range of microorganisms and can be viewed as an alternative for the treatment of microbial infections. In this work we synthesized "first" and "second" generation photosensitizers (PSs), the tetra-cationic porphyrin and the new penta-cationic chlorin , respectively, and evaluated their efficiency against two antibiotic resistant bacterial strains, Staphylococcus aureus and Pseudomonas aeruginosa. The PS was obtained in very good yield by an easy synthesis method. The PDI studies were performed in parallel with 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porphyrin tetra-iodide (), a widely studied PS in PDI, and the obtained results were compared. Two different light ranges were used: white light (400-800 nm) and red light (530-800 nm) delivered at a fluence rate of 150 mW cm(-2). The results show that both strains, even though antibiotic resistant, were efficiently inactivated by the three PSs, chlorin being the most effective. For the Gram positive bacterium S. aureus a 7.0 log reduction was observed after 5-10 min of irradiation, at a concentration of 0.5 μM, whereas for the Gram negative P. aeruginosa, similar photoinactivation occurred at a higher PS concentration (10 μM) and after a longer irradiation period (30 min). The synthetic chlorin can be regarded as promising for the treatment of bacterial infections under red light, which penetrates deeper in living tissues. The results of this study open the possibility to prepare a new series of chlorin-type derivatives to efficiently photoinactivate Gram (+) and (-) antibiotic resistant bacteria. The efficient PDI with the chlorin indicates high potential for the use of a scaffold in the preparation of new generation PSs based on cationic chlorin derivatives.

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“…In the context of new applications, a study investigated the antimicrobial effect of liposome enriched mTHPC for the use of Foscan in periodontal diseases. Enterococcus faecalis could be photoinactivated with 50 M mTHPC and 100 J.cm -2 indicating the possibility of adjuvant treatment of endodontic infections [21]. Related investigations include studies on the mechanism of cell death [22], the suppression of dark toxicity in PLGA nanoparticles [23], invasosomes for skin delivery of temoporfin [24], amongst others.…”

Section: Simple Modifications and Formulations Of Mthpcmentioning

confidence: 99%

mTHPC – A drug on its way from second to third generation photosensitizer?

Senge

2012

Photodiagnosis and Photodynamic Therapy

111

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Photodynamic suppression of Enterococcus faecalis using the photosensitizer mTHPC

Abstract: The results show that aPDT using the PS mTHPC incorporated in liposomes could be a new approach to adjuvant treatment of endodontic infections with E. faecalis.

Cited by 44 publications

References 64 publications

Antimicrobial Photodynamic Therapy for the Treatment of Teeth with Apical Periodontitis: A Histopathological Evaluation

Antimicrobial Photodynamic Therapy for the Treatment of Teeth with Apical Periodontitis: A Histopathological Evaluation

Comparative photodynamic inactivation of antibiotic resistant bacteria by first and second generation cationic photosensitizers

mTHPC – A drug on its way from second to third generation photosensitizer?

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