Photodynamic therapy and combinatory treatments for the control of biofilm-associated infections

Ribeiro, Marta F. T.; Gomes, Inês B.; Saavedra, Maria José; Simões, Manuel

doi:10.1111/lam.13762

Cited by 21 publications

(5 citation statements)

References 105 publications

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“…On the contrary, it has demonstrated high potential for addressing localized infections sustained by MDR bacteria including hospital-acquired pathogens like E. faecium, S. aureus, K. pneumoniae, A. baumannii, P. aeruginosa, and Enterobacter spp., which together constitute the group ESKAPE [137]. Additionally, in vitro and in vivo studies have demonstrated the capability of PDT of eradicating or significantly reducing biofilms, thus finding applications in dental diseases, skin infections and orthopedic implants treatment [138,139]. The ongoing advancement of antibacterial PDT systems is also marked by the continuous refinement of the strategies, prominently through synergistic combinations of diverse chemicals.…”

Section: Clinical Trialsmentioning

confidence: 99%

Reactive Oxygen Species (ROS)-Mediated Antibacterial Oxidative Therapies: Available Methods to Generate ROS and a Novel Option Proposal

Alfei,

Schito,

Schito

et al. 2024

Preprint

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The increasing emergence of multidrug resistant (MDR) pathogens causes difficult-to-treat infections, with long-term hospitalizations and high incidence of death, thus representing a global public health problem. To manage MDR bacteria bugs, new antimicrobial strategies are necessary, and their introduction in practice is a daily challenge for scientists in the field. An extensively studied approach to treat MDR infections consists in inducing high levels of reactive oxygen species (ROS) by several methods. Although further clinical investigations are mandatory on the possible toxic effects of ROS to mammalian cells, clinical evaluations are extremely promising, and their topical use to treat infected wounds and ulcers, also in presence of biofilm, is already clinically approved. Biochar (BC) is a carbonaceous material obtained by pyrolysis of different vegetable and animal biomass feedstocks, at 200−1000 °C in limited presence of O2. Recently, it has been demonstrated that BC capability of removing organic and inorganic xenobiotics is mainly due to the presence of persistent free radicals (PFRs), which can activate oxygen, H2O2 or persulfate in the presence or not of transition metals by electron transfer thus generating ROS, which in turn degrade pollutants by advanced oxidation processes (AOPs). In this context, the antibacterial effects of BC containing PFRs have been demonstrated by some authors against Escherichia coli and Staphylococcus aureus, thus giving birth to our idea of the possible use of BC-derived PFRs as a novel method capable of inducing ROS generation for antimicrobial oxidative therapy. Here, the general aspects concerning ROS physiological and pathological production and regulation, and the mechanism by which they could exert antimicrobial effects have been reviewed. The methods currently adopted to induce ROS production for antimicrobial oxidative therapy have been discussed. Finally, for the first time, BC-related PFRs have been proposed as a new source of ROS for antimicrobial therapy via AOPs.

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Section: Clinical Trialsmentioning

confidence: 99%

Reactive Oxygen Species (ROS)-Mediated Antibacterial Oxidative Therapies: Available Methods to Generate ROS and a Novel Option Proposal

Alfei,

Schito,

Schito

et al. 2024

Preprint

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“…Generally, aPDI should be an effective method for the eradication of a wide range of microorganisms, including both gram-positive and gram-negative bacteria, viruses, fungi, and parasites [ 26 , 27 , 28 , 199 , 200 , 201 ]. Due to the fact that PDI is multi-targeted, microorganisms are not able to develop resistance [ 202 , 203 ]. Moreover, PDI is highly effective against microorganisms resistant to conventional antimicrobials [ 204 , 205 , 206 , 207 , 208 ].…”

Section: Principles Of Apdt and The Use Of G Mellonella ...mentioning

confidence: 99%

Galleria mellonella—A Model for the Study of aPDT—Prospects and Drawbacks

Bugyna,

Kendra,

Bujdáková

2023

Microorganisms

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Galleria mellonella is a promising in vivo model insect used for microbiological, medical, and pharmacological research. It provides a platform for testing the biocompatibility of various compounds and the kinetics of survival after an infection followed by subsequent treatment, and for the evaluation of various parameters during treatment, including the host–pathogen interaction. There are some similarities in the development of pathologies with mammals. However, a limitation is the lack of adaptive immune response. Antimicrobial photodynamic therapy (aPDT) is an alternative approach for combating microbial infections, including biofilm-associated ones. aPDT is effective against Gram-positive and Gram-negative bacteria, viruses, fungi, and parasites, regardless of whether they are resistant to conventional treatment. The main idea of this comprehensive review was to collect information on the use of G. mellonella in aPDT. It provides a collection of references published in the last 10 years from this area of research, complemented by some practical experiences of the authors of this review. Additionally, the review summarizes in brief information on the G. mellonella model, its advantages and methods used in the processing of material from these larvae, as well as basic knowledge of the principles of aPDT.

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“…First, the multitarget nature of aPDT, responsible for the elimination of microbial organisms, reduces the chances of resistance development . Additionally, the treatment remains efficient regardless of the microbial antibiotic resistance profile. ,,, Moreover, the photodynamic approach can be successfully used to combat bacteria but also viruses, fungi and parasites. , …”

Section: Introductionmentioning

confidence: 99%

“…6,8,17,18 Moreover, the photodynamic approach can be successfully used to combat bacteria but also viruses, fungi and parasites. 10,19 The efficacy of photodynamic action is significantly related on the structure of the PS and its ability to produce ROS. While various classes of organic compounds have been explored as PS in aPDT, porphyrins and related macrocycles stand out as prime candidates.…”

Section: Introductionmentioning

confidence: 99%

Can Porphyrin–Triphenylphosphonium Conjugates Enhance the Photosensitizer Performance Toward Bacterial Strains?

Chaves,

Morais,

Vieira

et al. 2024

ACS Appl. Bio Mater.

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Antimicrobial photodynamic treatment (aPDT) offers an alternative option for combating microbial pathogens, and in this way, addressing the challenges of growing antimicrobial resistance. In this promising and effective approach, cationic porphyrins and related macrocycles have emerged as leading photosensitizers (PS) for aPDT. In general, their preparation occurs via N-alkylation of nitrogen-based moieties with alkyl halides, which limits the ability to fine-tune the features of porphyrin-based PS. Herein, is reported that the conjugation of porphyrin macrocycles with triphenylphosphonium units created a series of effective cationic porphyrinbased PS for aPDT. The presence of positive charges at both the porphyrin macrocycle and triphenylphosphonium moieties significantly enhances the photodynamic activity of porphyrin-based PS against both Gram-positive and Gram-negative bacterial strains. Moreover, bacterial photoinactivation is achieved with a notable reduction in irradiation time, exceeding 50%, compared to 5,10,15,20-tetrakis(1-methylpyridinium-4yl)porphyrin (TMPyP), used as the reference and known as good PS. The improved capability of the porphyrin macrocycle to generate singlet oxygen combined with the enhanced membrane interaction promoted by the presence of triphenylphosphonium moieties represents a promising approach to developing porphyrin-based PS with enhanced photosensitizing activity.

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Photodynamic therapy and combinatory treatments for the control of biofilm-associated infections

Abstract: Significance and Impact of the Study: Photodynamic therapy is highly effective in the control of microbial growth and has potential to provide solutions for the antibiotic crises.

Cited by 21 publications

References 105 publications

Reactive Oxygen Species (ROS)-Mediated Antibacterial Oxidative Therapies: Available Methods to Generate ROS and a Novel Option Proposal

Reactive Oxygen Species (ROS)-Mediated Antibacterial Oxidative Therapies: Available Methods to Generate ROS and a Novel Option Proposal

Galleria mellonella—A Model for the Study of aPDT—Prospects and Drawbacks

Can Porphyrin–Triphenylphosphonium Conjugates Enhance the Photosensitizer Performance Toward Bacterial Strains?

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