Photodynamic therapy for human oral squamous cell carcinoma and xenografts using a new photosensitizer, PAD‐S31

Date, Masataka; Sakata, Isao; Fukuchi, K.; Ohura, Kiyoshi; Azuma, Yasutaka; Shinohara, Mitsuko; Matsuzaki, Koichi; Namiki, Yoshihisa; Takahashi, Hiroshi

doi:10.1002/lsm.10188

Cited by 12 publications

(15 citation statements)

References 25 publications

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“…age‐related macular degeneration) (Huang, ). Photodynamic therapy involves the use of photosensitizers (PS), which accumulate in tumours and are subsequently activated by visible light of wavelength matched to their absorption spectra (Date et al ., ). Low systemic toxicity and the ability to selectively destroy tumours accessible to light are the advantages of PDT over the other conventional cancer therapies.…”

Section: Introductionmentioning

confidence: 97%

Photodynamic Therapy of Pheophorbide a Inhibits the Proliferation of Human Breast Tumour via Both Caspase‐dependent and ‐independent Apoptotic Pathways in In Vitro and In Vivo Models

Hoi

Wong

Chan

et al. 2011

Phytotherapy Research

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Breast cancer is conventionally treated by surgery and radiotherapy, with adjuvant chemotherapy and hormonotherapy as supplementary treatments. However, such treatments are associated with adverse side effects and drug resistance. In this study, Pheophorbide a (Pa), a photosensitizer isolated from Scutelleria barbata, was analysed for its antiproliferative effect on human breast tumour cells. The IC (inhibitory concentration)(50) of the combined treatment of Pa and photodynamic therapy (Pa-PDT) on human breast tumour MCF-7 cells was 0.5 µm. Mechanistic studies in MCF-7 cells demonstrated that Pa was localized in the mitochondria, and reactive oxygen species were found to be released after Pa-PDT. Apoptosis was the major mechanism responsible for the tumour cell death, and mitochondrial membrane depolarization and cytochrome c release highlighted the role of mitochondria in the apoptotic mechanism. Up-regulation of tumour suppressor protein p53, cleavage of caspase-9 and poly (ADP-ribose) polymerase suggested that the caspase-dependent pathway was induced, while the release of apoptosis-inducing factors demonstrated that the apoptosis was also mediated by the caspase-independent mechanism. In vivo study using the mouse xenograft model showed a significant inhibition of MCF-7 tumour growth by Pa-PDT. Together, the results of this study provide a basis for understanding and developing Pa-PDT as a cure for breast cancer.

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Section: Introductionmentioning

confidence: 97%

Photodynamic Therapy of Pheophorbide a Inhibits the Proliferation of Human Breast Tumour via Both Caspase‐dependent and ‐independent Apoptotic Pathways in In Vitro and In Vivo Models

Hoi

Wong

Chan

et al. 2011

Phytotherapy Research

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“…Photodynamic therapy (PDT) is a promising cancer treatment in which a photosensitizing drug accumulates in tumors and is subsequently activated by visible light of an appropriate wavelength matched to the absorption (Date et al , 2003). The advantages of this method, as compared to other conventional cancer treatment modalities, are its low systemic toxicity and its ability to destroy tumors selectively (Gaullier et al , 1997).…”

Section: Introductionmentioning

confidence: 99%

Influence of administration methods on the accumulation of ALA‐induced Pp‐IX in mouse tongue tumors

et al. 2006

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“…Therefore, deep and large tumors may not be able to be treated using this method. It has been recognized that the method is only efficient on tumors with a depth of ≤1.0 cm (11,12). …”

Section: Discussionmentioning

confidence: 99%

Efficacy of krypton laser photodynamic therapy for oral mucosa dysplasia in 9,10-dimethyl-1,2-benzanthracene-treated hamsters

Shen

et al. 2013

Oncology Letters

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The present study aimed to evaluate the efficacy of krypton laser photodynamic therapy (PDT) with PsD-007 for the treatment of oral mucosa dysplasia in 9,10-dimethyl-1,2-benzanthracene (DMBA)-treated hamsters. A DMBA-induced hamster cheek pouch model of precancerous lesions was created and the resultant 25 hamsters were divided into five groups. The right side was treated with PDT and the left side was used as the positive control. Following systemic anesthesia, an incision was made in the groin area to expose the femoral vein. PsD-007 was administered intravenously through the femoral vein. Various doses of photosensitizer were used to treat groups A–E. Subsequent to closing the incision, the right side of the buccal mucosa was irradiated with light using the krypton laser at a wavelength of 413 nm, a power density of 150 mW/cm2 and an irradiation time of 20 min. At six weeks post-surgery, the response was analyzed using histological examinations of the buccal pouch mucosa. A total of 24 hamsters completed the six-week observation period, as one hamster from group C died in the second week following the PDT. Of all 24 irradiated sides, 15 formed normal mucosal tissues and nine demonstrated mild dysplasia. Of the total control sides, six developed moderate dysplasia, five developed severe dysplasia and 13 progressed to carcinoma in situ or squamous cell carcinoma (SCC). The results revealed a significant difference between the two sides (P<0.01) and the various doses of the PsD-007 groups. When the PsD-007 dose was >10 mg/kg, there was no statistical difference (P>0.05). PsD-007-mediated krypton laser PDT is effective for the treatment of oral mucosa dysplasia in hamsters.

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Photodynamic therapy for human oral squamous cell carcinoma and xenografts using a new photosensitizer, PAD‐S31

Abstract: The present study demonstrates that PAD-S31 may serve as a potent photosensitizer for PDT. Furthermore, it is expected that this therapy will be clinically useful for the treatment of patients with oral carcinoma.

Cited by 12 publications

References 25 publications

Photodynamic Therapy of Pheophorbide a Inhibits the Proliferation of Human Breast Tumour via Both Caspase‐dependent and ‐independent Apoptotic Pathways in In Vitro and In Vivo Models

Photodynamic Therapy of Pheophorbide a Inhibits the Proliferation of Human Breast Tumour via Both Caspase‐dependent and ‐independent Apoptotic Pathways in In Vitro and In Vivo Models

Influence of administration methods on the accumulation of ALA‐induced Pp‐IX in mouse tongue tumors

Efficacy of krypton laser photodynamic therapy for oral mucosa dysplasia in 9,10-dimethyl-1,2-benzanthracene-treated hamsters

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