Photodynamic therapy in dermatology

Steinbauer, Julia; Schreml, Stephan; Kohl, Elisabeth; Karrer, Sigrid; Landthaler, Míchael; Szeimies, Rolf‐Markus

doi:10.1111/j.1610-0387.2010.07343.x

Cited by 23 publications

(32 citation statements)

References 22 publications

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“…Eine klinisch etablierte Kombinationsbehandlung aus Laserlicht oder IPL und topisch applizierter Aminolävulinsäure (ALA) ist die photodynamische Therapie (PDT; [49,50,51]). Eine Indikation, die in den letzten Jahren klinisch und auch wissenschaftlich zunehmend an Bedeutung gewinnt, ist die photodynamische Photorejuvenation, also der Einsatz der PDT zur Behandlung der Hautalterung.…”

Section: Photodynamische Therapieunclassified

Neue Entwicklungen in der Lasertherapie

Babilas

Landthaler

2012

Hautarzt

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Based on the theory of stimulated emission of radiation that was proposed by Albert Einstein in 1916, the first lasers were developed in the 1960s. The first clinical use of laser technology in a German university took place in 1978 in the Department of Dermatology of the Ludwig-Maximilian-University in Munich under the guidance of the former director, Prof. Dr. med. Dr. h.c. mult. Otto Braun-Falco. In the following years, laser technology developed rapidly. Today laser technology is a widely used interdisciplinary therapeutic procedure that has deep clinical and scientific roots in dermatology. There are many conditions in both classic and aesthetic dermatology that are routinely - and sometimes exclusively - treated with lasers. Here we review recent developments in laser medicine. There seems to be a trend to combination procedures. To enhance efficacy, different laser systems are together or lasers are combined with specific topical medications.

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Section: Photodynamische Therapieunclassified

Neue Entwicklungen in der Lasertherapie

Babilas

Landthaler

2012

Hautarzt

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“…A photosensitizer in the excited state causes photochemical reactions that lead to necrosis and apoptosis in pathological tissues. For many years PDT was used to detect and to treat different lesions located in the skin and mucosa [9–13]. Porphyrins are the most commonly used photosensitizers.…”

Section: Introductionmentioning

confidence: 99%

The application of Levulan®-based photodynamic therapy with imiquimod in the treatment of recurrent basal cell carcinoma

2012

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SummaryBackgroundCommon skin tumors like basal- and squamous-cell carcinoma present a serious problem in modern medicine. Exposure to ultraviolet solar radiation is the main cause of these lesions. Since application of Aldara® and PDT separately is well documented, we decided to use both methods together.The aim of our study was to evaluate the effectiveness of local photodynamic therapy supplemented with topical application of Aldara® in basal-cell carcinoma.Material/MethodsThirty-four patients ages 50 to 68 years were enrolled to the trial and underwent PDT treatment. Each case of BCC was histopathologically confirmed. Ten patients were subjected to local Levulan®-PDT and placebo (Eucerin as vehicle cream), and 24 patients were subjected to Levulan®-PDT and imiquimod. Photodynamic diagnosis (PDD) was used to detect and visualize suspicious foci (including cancer lesions).ResultsIn the group of patients who were treated using Levulan®-PDT and placebo, 6 patients (60%) were totally cured and 4 lesions (40%) significantly decreased in size. In the group of patients treated with Levulan®-PDT and imiquimod, 18 lesions totally disappeared (75%), 6 lesions significantly diminished, and in 1 patient small foci of previously excised BCC developed again in scar tissue 10 month after the first control examination.ConclusionsCure was achieved without any scarring and with very good cosmetic effects. Although this is the preliminary report, the presented modification of PDT seems to be reasonable and promising in treating basal-cell carcinoma.

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“…Photodynamic therapy (PDT) is a new method of tumor treatment which was developed in the 1980s. The principle of PDT is as follows: a photosensitizer can be selectively accumulated in the tumor, and then activated using a laser of specific wavelength, thus producing a singlet oxygen and oxygen radical, which can destroy the tumor tissues [3] . PDT has been mainly used to treat tumors on the body surface or inside the respiratory tract and digestive canal.…”

Section: Introductionmentioning

confidence: 99%

In vitro lethal effect of photodynamic therapy on human pancreatic cancer cells and its major influencing factors

Zhong

Huang

et al. 2011

Clin. Oncol. Cancer Res.

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OBJECTIVETo investigate the in vitro lethal effect of photodynamic therapy (PDT) using the photosensitizer hematoporphyrin on the human pancreatic cancer cell line Panc-1, the major influencing factors and the mechanisms of treatment. METHODS Three factors-the time needed for photosensitizer and cell incubation, the photosensitizer concentration (PhoC) and the exposure dose (ExpD)-were examined with different levels of these factors. Optical density (OD) was used as a measure of CCK-8 in the experiment, and was converted to the rate of cell survival. The separate effect of each factor on the photodynamic action was studied, and the interactions were investigated. The effects of different incubation times and PhoC levels on the fluorescence intensity (FI) of the intracellular photosensitizer were determined, and the mechanisms of these factors leading to the therapeutic effects of PDT discussed. RESULTSAn increase in the photosensitizer and cell incubation time, an increase of PhoC, and enhancement of the ExpD, produced a corresponding decrease in the rate of Panc-1 cell survival after PDT (P , 0.05). PDT achieved its maximum lethal effects 16 h after starting the incubation, with a PhoC of 10 mg/L and an ExpD of 20 J/cm 2 ; at these levels a synergistic interaction between PhoC and the ExpD occurred, decreasing the cell survival rate (P , 0.05). Neither simple administration of photosensitizer without ExpD (0 J/cm 2 ) or illumination in the absence of PhoC (0 mg/L) affected the rate of cell survival (P . 0.05). With an increase of PhoC and lengthening of the incubation time, the FI of the intracellular photosensitizer accordingly increased (P , 0.05), and attained its maximum value at a PhoC of 10 mg/L and 36 h after the incubation. With an increase of PhoC, the FI of the photosensitizer, hematoporphyrin, in the solution increased progressively at first and then decreased (fluorescence quenching). CONCLUSIONPDT with the photosensitizer hematoporphyrin has clear lethal effects on the human pancreatic cancer cell line Panc-1, but the presence of a photosensitizer and laser irradiation by themselves do not have independent lethal effects. The three influencing factors-the time for photosensitizer and cell incubation, PhoC and ExpD-correlate positively with the PDT response, within certain limits. Beyond these limits, the PDT response does not significantly increase. The main mechanism of the PDT response lies in the effect of these factors on the level of the intracellular photosensitizer and the fluorescence quenching of the photosensitizer. A synergistic effect exists between PhoC and ExpD.

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Photodynamic therapy in dermatology

Cited by 23 publications

References 22 publications

Neue Entwicklungen in der Lasertherapie

Neue Entwicklungen in der Lasertherapie

The application of Levulan®-based photodynamic therapy with imiquimod in the treatment of recurrent basal cell carcinoma

In vitro lethal effect of photodynamic therapy on human pancreatic cancer cells and its major influencing factors

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