Photodynamic therapy of the experimental tumors of different morphological types with liposomal boronated chlorin е6

Abstract

The article summarizes the results of studies of the effectiveness of photodynamic therapy using a new domestic photosensitizer liposomal borated chlorin e6 (LBC) after its parenteral administration (intraperitoneal and intravenous). Antitumor efficacy was evaluated in rats with M-1 sarcoma and PC-1 alveolar liver cancer and mice with B16 melanoma and Ehrlich’s carcinoma, which were transplanted subcutaneously into the thigh area of the animals. The aim of the study was to determine the optimal regimes of photodynamic therapy that would allow achieving the maximum antitumor effect up to 21 days after the photodynamic therapy. The therapy was carried out under the control of the accumulation of the photosensitizer in the tumor and surrounding tissues of the thigh by selecting the doses of the drug and the parameters of laser radiation (energy density and power density). The effectiveness of therapy was assessed by the inhibition of tumor growth, by the percentage of animals with complete tumor regression, by the absolute growth rate in animals with continued tumor growth compared to controls. The results of our studies have shown that the domestic photosensitizer liposomal borated chlorin e6 has high antitumor activity in vivo. In an experimental study of the photosensitizer under certain PDT modes, the maximum antitumor effect (complete tumor regression in 100% of animals) was obtained up to 21 days after PDT in all tumor models used.

On the Pathomorphological Pattern of the Efficiency of Photodynamic Therapy of Murine Melanoma B16 Using a New Photosensitizer Based on Chlorin e6 Conjugate with a Prostate-Specific Membrane Antigen

“…On the same day, a session of photodynamic therapy was performed, as described previously [ 10 ]; the mice of the experimental groups received the PS in doses of 2.5 and 5.0 mg/kg into the caudal vein. The tumors were exposed to the laser irradiation using an Atkus-2 semiconductor device (Atkus, Russia; λ = 662 nm, diameter of the light spot = 1 cm).…”

Photodynamic Therapy of Experimental Tumors of Laboratory Animals with a New Photosensitizer Heliochlorin

“…Асцитическую жидкость карциномы Эрлиха от мышей доноров вводили подкожно в область бедра 24 половозрелым самкам массой 20-25 г, для воспроизведения солидной опухоли. [13][14][15] Саркому М-1 перевивали 18 половозрелым самкам крыс массой тела 160-190 г под кожу в область бедра кусочками опухоли донора. [16] На 3 сутки после перевивки при достижении наибольшего диаметра опухолевых узлов у мышей (0,4-0,6 см) и на 7-9 сутки при достижении диаметра саркомы крыс (0,7-1,0 см), животных методом рандомизации распределяли на опытные и контрольные группы (Таблица 1).…”