Photodynamic therapy on keloid fibroblasts in tissue-engineered keratinocyte-fibroblast co-culture

Chiu, Lynn; Sun, Chung Ho; Yeh, Alvin T.; Torkian, Behrooz A.; Karamzadeh, Amir M.; Tromberg, Bruce J.; Wong, Brian J. F.

doi:10.1002/lsm.20213

Cited by 52 publications

(27 citation statements)

References 61 publications

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“…Chemical compounds such as hematoporphyrin (34), porphyrin derivatives (35,36), phenothiazinium salts (19,37), chlorin (18,38) and 5‐aminolaevulinic acid‐induced porphyrin sensitizers (37,39) were studied and found to demonstrate high bactericidal effect against S. aureus strains after illumination with visible light. The cytotoxicity of many described above sensitizers toward keratinocytes and fibroblasts was also studied (36,40–45). Zeina et al.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Role of the Pharmacological Inhibition of Staphylococcus aureus Multidrug Resistance Pumps and the Variable Levels of the Uptake of the Sensitizer in the Strain‐Dependent Response of Staphylococcus aureus to PPArg₂‐Based Photodynamic Inactivation

Grinholc¹,

Zawacka-Pankau²,

Gwizdek-Wiśniewska

et al. 2010

Photochem & Photobiology

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The emergence of antibiotic resistance among pathogenic bacteria has caused an urgent need for the development of alternative therapeutics. One possibility is a combination of nontoxic photosensitizers (PS) and visible light, recognized as photodynamic therapy. Although it is known that Staphylococcus aureus is susceptible to photodynamic inactivation (PDI), the factors that determine the emerging variation among strains in the response to the treatment remain unclear. Some data indicate that cationic photosensitizing dyes such as phenothiaziniums which vary a lot in the chemical structure might target multidrug resistance pumps. In this study, we analyzed whether the uptake and activity of the multidrug resistance pumps might influence the previously observed variations among the clinical strains to protoporphyrin-derived, amphipilic protoporphyrin diarginate-mediated photodynamic treatment (12 J cm(-2) ). Using a new set of four additionally selected methicillin-resistant and methicillin-susceptible clinical as well as ATCC S. aureus strains we confirmed that the bactericidal effect of the PDI is strain-dependent as it ranged from 0 to 5 log(10) -unit reduction in viable counts. However, neither the variable levels of the uptaken PS nor the pharmacological inhibition of NorA efflux pump explained such a phenomenon.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Role of the Pharmacological Inhibition of Staphylococcus aureus Multidrug Resistance Pumps and the Variable Levels of the Uptake of the Sensitizer in the Strain‐Dependent Response of Staphylococcus aureus to PPArg₂‐Based Photodynamic Inactivation

Grinholc¹,

Zawacka-Pankau²,

Gwizdek-Wiśniewska

et al. 2010

Photochem & Photobiology

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“…Therefore, in order to consider the antifungal PDT as a clinical treatment for denture‐induced stomatitis, it is necessary to know its antifungal potential, as well as its cytotoxic effect on the individual's normal cells. The effect of PDT with some photosensitizers on keratinocyte and fibroblast cultures has been demonstrated by several in vitro studies (Zeina et al, 2002; Zeina et al, 2003; Chiu et al, 2005; Lambrechts et al, 2005b). However, the toxicity of Photogem®, a first‐generation haematoporphyrin‐derived photosensitizer, associated with LEDs (light‐emitting diodes) on the normal cells has not yet been described.…”

Section: Introductionmentioning

confidence: 99%

Photodynamic therapy associating Photogem® and blue LED on L929 and MDPC‐23 cell culture

Ribeiro

Pavarina

Trindade

et al. 2010

Cell Biology International

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To evaluate the cytotoxicity of PDT (photodynamic therapy) with Photogem associated to blue LED (light-emitting diode) on L929 and MDPC-23 cell cultures, 30000 cells/cm2 were seeded in 24-well plates for 48 h, incubated with Photogem (10, 25 or 50 mg/l) and irradiated with an LED source (460+/-3 nm; 22 mW/cm2) at two energy densities (25.5 or 37.5 J/cm2). Cell metabolism was evaluated by the MTT (methyltetrazolium) assay (Dunnet's post hoc tests) and cell morphology by SEM (scanning electron microscopy). Flow cytometry analysed the type of PDT-induced cell death as well and estimated intracellular production of ROS (reactive oxygen species). There was a statistically significant decrease of mitochondrial activity (90% to 97%) for all Photogem concentrations associated to blue LED, regardless of irradiation time. It was also demonstrated that the mitochondrial activity was not recovered after 12 or 24 h, characterizing irreversible cell damage. PDT-treated cells presented an altered morphology with ill-defined limits. In both cell lines, there was a predominance of necrotic cell death and the presence of Photogem or irradiation increased the intracellular levels of ROS. PDT caused severe toxic effects in normal cell culture, characterized by the reduction of the mitochondrial activity, morphological alterations and induction of necrotic cell death.

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“…For the TPEF and SHG imaging studies, we used stained organotypic tissue models (RAFTs) 8. They consisted of human dermal fibroblast cells dispersed in type-1 rat-tail collagen.…”

mentioning

confidence: 99%

Effect of excitation wavelength on penetration depth in nonlinear optical microscopy of turbid media

et al. 2009

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Abstract. We present a comparative study of two-photon excited fluorescence ͑TPEF͒ and second harmonic generation ͑SHG͒ imaging in turbid media at 800-and 1300-nm excitation. The depth-dependent decay of TPEF and SHG signals in turbid tissue phantoms is used to estimate the impact of light scattering on excitation intensity at each wavelength. A 50 to 80% increase in scattering length is observed using 1300-nm excitation, while peak TPEF emission intensity is obtained 10 to 20 m beneath the surface for both sources. The increased penetration depth at 1300 nm is confirmed by TPEF and SHG microscopy of tissue phantoms composed of gelatin/microspheres and 3-D organotypic collagen-fibroblast cultures, respectively. Our results establish the feasibility of 1.3-m excitation in nonlinear optical microscopy.

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Photodynamic therapy on keloid fibroblasts in tissue-engineered keratinocyte-fibroblast co-culture

Abstract: A PDT dosimetry range was established, which reduces tissue contraction and collagen density while minimizing injury to fibroblasts.

Cited by 52 publications

References 61 publications

Evaluation of the Role of the Pharmacological Inhibition of Staphylococcus aureus Multidrug Resistance Pumps and the Variable Levels of the Uptake of the Sensitizer in the Strain‐Dependent Response of Staphylococcus aureus to PPArg₂‐Based Photodynamic Inactivation

Evaluation of the Role of the Pharmacological Inhibition of Staphylococcus aureus Multidrug Resistance Pumps and the Variable Levels of the Uptake of the Sensitizer in the Strain‐Dependent Response of Staphylococcus aureus to PPArg₂‐Based Photodynamic Inactivation

Photodynamic therapy associating Photogem® and blue LED on L929 and MDPC‐23 cell culture

Effect of excitation wavelength on penetration depth in nonlinear optical microscopy of turbid media

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Photodynamic therapy on keloid fibroblasts in tissue-engineered keratinocyte-fibroblast co-culture

Abstract: A PDT dosimetry range was established, which reduces tissue contraction and collagen density while minimizing injury to fibroblasts.

Cited by 52 publications

References 61 publications

Evaluation of the Role of the Pharmacological Inhibition of Staphylococcus aureus Multidrug Resistance Pumps and the Variable Levels of the Uptake of the Sensitizer in the Strain‐Dependent Response of Staphylococcus aureus to PPArg2‐Based Photodynamic Inactivation

Evaluation of the Role of the Pharmacological Inhibition of Staphylococcus aureus Multidrug Resistance Pumps and the Variable Levels of the Uptake of the Sensitizer in the Strain‐Dependent Response of Staphylococcus aureus to PPArg2‐Based Photodynamic Inactivation

Photodynamic therapy associating Photogem® and blue LED on L929 and MDPC‐23 cell culture

Effect of excitation wavelength on penetration depth in nonlinear optical microscopy of turbid media

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Evaluation of the Role of the Pharmacological Inhibition of Staphylococcus aureus Multidrug Resistance Pumps and the Variable Levels of the Uptake of the Sensitizer in the Strain‐Dependent Response of Staphylococcus aureus to PPArg₂‐Based Photodynamic Inactivation

Evaluation of the Role of the Pharmacological Inhibition of Staphylococcus aureus Multidrug Resistance Pumps and the Variable Levels of the Uptake of the Sensitizer in the Strain‐Dependent Response of Staphylococcus aureus to PPArg₂‐Based Photodynamic Inactivation