Photodynamic therapy on the ultrastructure of glioma cell

Hu, Shaoshan; Zhang, Ruyou; Zheng, Yongri

doi:10.1117/12.639223

Cited by 12 publications

(14 citation statements)

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“…The most significant damage was found in mitochondria, as reported before [19,22] . Swelling occurred in the cellular organs such as mitochondria and endoplasmic reticula in most cells treated with ALA alone or ALA plus iron chelators.…”

Section: Morphological Changesmentioning

confidence: 56%

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A comparative study on the enhancement efficacy of specific and non-specific iron chelators for protoporphyrin IX production and photosensitization in HaCat cells

Xia

Huang

Lin

et al. 2009

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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The iron chelators can be utilized in target cells to improve 5-aminolaevulinic acid (ALA)-based photodynamic therapy (PDT). The purpose of this study is to compare the effect of two kinds of iron chelators, desferrioxamine (DFO) and ethylenediaminetetraacetic acid (EDTA) on the enhancement of ALA-PDT. HaCat cells were cultured in medium containing 2.0 mmol/L of ALA and 0.5 mmol/L of DFO or EDTA. After 3-h incubation in the dark, the concentration of cellular protoporphyrin IX (PpIX) was detected by high performance liquid chromatography (HPLC), and the fluorescence of PpIX was observed at 630 nm emission under confocal laser scanning microscope. For PDT, HaCat cells were irradiated using 632.8 nm laser, and the fractions of apoptotic and necrotic cells were flow cytometrically assayed. Related differences in morphology and ultrastructure of Ha-Cat cells were observed using optical microscope or transmission electron microscope. Compared to incubation with ALA alone, the addition of DFO or EDTA increased the concentration of cellular PpIX and the fluorescent density of PpIX, and also increased cell death ratio after PDT. PDT using ALA plus DFO produced the highest cellular PpIX level, greatest cell death ratio and most severe structural damage to the cells. It was concluded that both DFO and EDTA could enhance ALA-based PpIX production and PDT. Compared to the non-specific iron chelator of EDTA, the specific chelator, DFO, showed more potential for the enhancement.

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Section: Morphological Changesmentioning

confidence: 56%

“…The sections were post-stained with uranyl acetate and lead hydroxide. The sub-cellular structure was observed under transmission electron microscope (Hitachi H600, Japan) [19] . Ultrathin sections were obtained by using ultramicrotome PowerTome-XL (RMC, USA).…”

Section: Morphological Observationmentioning

confidence: 99%

A comparative study on the enhancement efficacy of specific and non-specific iron chelators for protoporphyrin IX production and photosensitization in HaCat cells

Xia

Huang

Lin

et al. 2009

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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“…Thus, glioma cells have the ability to take up photosensitizers. In an in vitro study, Hu et al (18) showed that HMME-mediated PDT could induce the apoptosis of C6 glioma cells and that calcium overload played an important role in extensive ultrastructural damage.…”

Section: Discussionmentioning

confidence: 99%

The inhibitory effect of photodynamic therapy and of an anti-VCAM-1 monoclonal antibody on the in vivo growth of C6 glioma xenografts

Zhan

2011

Braz J Med Biol Res

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“…PDT's effect on the cultured glioma cells has been reported (Chopp et al, 1996;Hu et al, 2007;Karmakar et al, 2007;Sailer et al, 2007). However, not only malignant but also normal glial cells and neurons are damaged during PDT of brain tumors.…”

mentioning

confidence: 98%

Dynamics of ultrastructural alterations in photosensitized crayfish glial and neuronal cells: Structures involved in transport processes and neuroglial interactions

Федоренко

Fedorenko

et al. 2010

J. Neurosci. Res.

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Photodynamic therapy (PDT) is used for cancer treatment, including brain tumors. To explore the dynamics of photodynamic injury of glial cells and neurons and corresponding neuroglial interactions, we studied ultrastructure of the PDT-treated crayfish stretch receptor that consists of a single sensory neuron enwrapped by glial cells. Just after PDT, swelling of some mitochondria, dictyosomes, and endoplasmic reticulum cisterns occurred in neurons and glial cells. Tubular lattices involved in intraglial transport became swollen and disintegrated. At 1 hr postirradiation, these alterations were expanded to the whole cells. Segregation of the neuronal cytoplasm by Nissl bodies, which were involved in protein synthesis and transport along neurites, was lost. Swelling of submembrane cisterns prevented formation of glial protrusions and double-wall vesicles involved in the glia-to-neuron transport. Five hours later, glial layers lost organelles, stuck together, or dilated locally as a result of edema. In the neuronal cytoplasm, only demises of ER and swollen mitochondria were present, but few mitochondria retained normal structure. Thus, swelling of intracellular organelles, the first sign of photodynamic injury, occurred simultaneously in neurons and glia, but glial organelles were eliminated more quickly. Therefore, glial cells were less resistant to PDT than neurons. Adjacent glial layers were damaged less than remote ones, suggesting their protection by the neuron. The structures involved in glia-to-neuron (neuronal submembrane cisterns, glial protrusions, double-wall vesicles), intraglial (tubular lattices), and intraneuronal (Nissl bodies, Golgi apparatus, microtubular bundles) transport were impaired at the earlier stages of stretch receptor damage.

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Photodynamic therapy on the ultrastructure of glioma cell

Cited by 12 publications

References 0 publications

A comparative study on the enhancement efficacy of specific and non-specific iron chelators for protoporphyrin IX production and photosensitization in HaCat cells

A comparative study on the enhancement efficacy of specific and non-specific iron chelators for protoporphyrin IX production and photosensitization in HaCat cells

The inhibitory effect of photodynamic therapy and of an anti-VCAM-1 monoclonal antibody on the in vivo growth of C6 glioma xenografts

Dynamics of ultrastructural alterations in photosensitized crayfish glial and neuronal cells: Structures involved in transport processes and neuroglial interactions

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