Photoelectric and Cinematographic Measurements of the Motility of Bull Sperm Cells

Rikmenspoel, Robert; Herpen, Gerard van

doi:10.1088/0031-9155/2/1/306

Cited by 45 publications

(41 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Focusing on the bovine sperm used in the present experiment, Rikmenspoel and Herpen (1957) indicated that sperm velocity is proportional to the frequency of the sperm rotation. Furthermore, they observed the elongation of the Contents lists available at ScienceDirect journal homepage: www.elsevier.com/locate/jbiomech www.JBiomech.com flagellum and suggested that bovine sperm rotates in three dimensions.…”

Section: Introductionmentioning

confidence: 82%

Effect of non-Newtonian fluid properties on bovine sperm motility

Hyakutake¹,

Suzuki²,

Yamamoto³

2015

Journal of Biomechanics

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 82%

Effect of non-Newtonian fluid properties on bovine sperm motility

Hyakutake¹,

Suzuki²,

Yamamoto³

2015

Journal of Biomechanics

View full text Add to dashboard Cite

“…A different tracking approach is the photoelectric method (Rikmenspoel, 1957;van Duijn & Rikmenspoel, 1960), results from which compared well with results from cine-microphotography. Unfortunately, relatively high dilu¬ tions (at least 40 for bull semen) are required in an optically very clear diluent (such as clarified egg yolk-citrate), and simultaneous recording from several apertures reduces measurement time to roughly 4 min.…”

Section: Introductionmentioning

confidence: 89%

Methods of Measuring Swimming Speed of Spermatozoa

Katz¹,

Dott²

1975

Reproduction

View full text Add to dashboard Cite

“…Regarding the bovine sperm, which is focused upon, in the present experiment, Rikmenspoel et al (1957) indicated that sperm velocity is proportional to the frequency of the sperm rotation. Furthermore, they observed the elongation of the flagellum and suggested that bovine sperm rotates in three dimensions.…”

Section: Introductionmentioning

confidence: 58%

Experimental study on the effect of a surrounding fluid on bovine sperm motility in three dimensions

Hyakutake

ORIHARA

Mezaki

2017

JBSE

View full text Add to dashboard Cite

We constructed an experimental system that can observe bovine sperm motility in three dimensions, simultaneously from the vertical and horizontal directions. The comparison of the experiments conducted using cover glass and square tubing indicates that the effect of the cover glass on the sperm motility is not negligible. Based on this result, we investigated a three-dimensional trajectory of a motile sperm in the square tubing by simultaneously observing through two high-speed cameras. In addition, we investigated the effect of different viscosities of the surrounding fluid on the sperm motility. The experimental results indicate that the increasing viscosity causes a decline in the sperm motility. Furthermore, we focus on the width of the sperm head and the flagellar shape. At low viscosity, the time variation of the head widths is relevant with the two directions and varies periodically. In contrast, at high viscosity, an almost constant value is maintained and periodical time variations are not observed for both directions. Moreover, a difference in the viscosity is observed for the tortuosity of the flagellum shape. These results suggest that the three-dimensional flagellar structure of the bovine sperm is a helix whose cross-sectional shape is an ellipse with quite a large aspect ratio. Further, the results state whether the rotation or nonrotation of the sperm is dependent on the balance between the viscous resistance acting on the sperm and the torque due to the flagellar shape. The experimental results obtained will be useful in clarifying the mechanics of sperm motility under their actual environmental conditions at high viscosity.

show abstract

Photoelectric and Cinematographic Measurements of the Motility of Bull Sperm Cells

Cited by 45 publications

References 4 publications

Effect of non-Newtonian fluid properties on bovine sperm motility

Effect of non-Newtonian fluid properties on bovine sperm motility

Methods of Measuring Swimming Speed of Spermatozoa

Experimental study on the effect of a surrounding fluid on bovine sperm motility in three dimensions

Contact Info

Product

Resources

About