Photoelectric Spectrophotometry. An Apparatus for the Ultra-violet and Visible Spectral Regions: Its Construction, Calibration, and Application to Chemical Problems

Hogness, T. R.; Zscheile, F. P.; Sidwell, A. E.

doi:10.1021/j150381a005

Cited by 128 publications

(43 citation statements)

References 6 publications

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“…No attempt was made to carry out a study of conformity to Beer's law, because the one solution in 1-and 2-cm thicknesses covered the photometric range of interest. However, it was known that Hogness, Zscheile, and Sidwell had made a run on exactly the same concentration used by von Halban and found good agreement with their curve [25] obtained from data on optimum concentration. From these data it was concluded that Beer's law held.…”

Section: Comparison Of Data With Those Of Other Observersmentioning

confidence: 56%

“…Tabular data on the spectral transmittancy of potassium chromate in 0.05 N potassium hydroxide have been published bv von Halban and Siedentopf [22], Rossler [23], Baly, Morton, and Riding [24], and Hogness, Zscheile, and Sidwell [25].…”

Section: Comparison Of Data With Those Of Other Observersmentioning

confidence: 99%

“…The potassium chromate was recrystallized four and five times. They show the absorption spectrum for this solution in a figure [25]. The dots through which the curve is drawn in their figure are taken from several series of determinations at 2.5-m/x intervals from 220 to 410 m/x.…”

Section: Comparison Of Data With Those Of Other Observersmentioning

confidence: 99%

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An Alkaline Solution of Potassium Chromate as a Transmittancy Standard in the Ultraviolet*

Haupt

1952

J. Opt. Soc. Am.

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The need for a means of testing the reliability of the photometric scale of spectrophotometers in the ultraviolet region (where glasses are unsuitable) has led to the study of an aqueous solution of potassium chromate having the composition 0.0400 g/liter of K 2 Cr04 in 0.05 N KOH. Based on extensive measurements, using photographic, photoelectric, and visual spectrophotometry, standard values of spectral transmittancy have been determined for 1.000 and 2.000 cm of solution at 25° C from 220 to 500 m/i. These values have been tabulated along with derived values of absorbancy, molar absorbancy index a M , and logiottM. The latter values have been compared graphically with those of other observers. Changes in spectral transmittancy with changes in temperature have been determined. The work has also included a study of the effects due to the container bottle and to age of solutions over periods up to 8 years, and a comparison of results obtained with the solution prepared either from K2OO4 or from K 2 Cr 2 07 as one of the reagents.

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Section: Comparison Of Data With Those Of Other Observersmentioning

confidence: 56%

Section: Comparison Of Data With Those Of Other Observersmentioning

confidence: 99%

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An Alkaline Solution of Potassium Chromate as a Transmittancy Standard in the Ultraviolet*

Haupt

1952

J. Opt. Soc. Am.

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“…The presence of urea is necessary to be sure that the heme can be freed from the protein ligands. For hemin no significant difference of absorbance was observed in the presence of urea; an extinction coefficient of 11.1 d -h m -1 was taken for the heme dicyanide complex [12].…”

Section: Heme-cyanide Complexmentioning

confidence: 99%

Molecular Weight and Quaternary Structure of Yeast l‐Lactate Dehydrogenase (Cytochrome b₂)

Pajot

Groudinsky

1970

European Journal of Biochemistry

108

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A new determination of the extinction coefficient of the Soret band of reduced cytochrome b2 provides a value of 183 mM−1cm−1 instead of the generally accepted figure of 232. Four methods were used: pyridine hemochromogen, dicyanide complex, spectrum in formic acid and iron titration.No significant differences are observed in the heme spectral properties of cytochrome b2 and its low molecular weight derivative “cytochrome b2 core”. Dry weight associated with iron and heme content determinations lead to a minimal molecular weight per heme of 58600. This result combined with hydrodynamic and crystallographic studies by other authors allows one to propose a tetrameric structure for cytochrome b2.

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“…The absorption coefficient obtained may also be influenced by the width of the spectral region isolated by the monochromator [see (2) for definition]. Analytical values for a representative plant extract remained constant under the following slit conditions: 6600 A slits 0.02-0.06 mm.…”

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confidence: 99%

ANALYSIS OF PLANT EXTRACTS FOR CHLOROPHYLLS a AND b BY A PHOTOELECTRIC SPECTROPHOTOMETRIC METHOD

Comar¹,

Zscheile²

1942

Plant Physiol.

Self Cite

205

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Photoelectric Spectrophotometry. An Apparatus for the Ultra-violet and Visible Spectral Regions: Its Construction, Calibration, and Application to Chemical Problems

Cited by 128 publications

References 6 publications

An Alkaline Solution of Potassium Chromate as a Transmittancy Standard in the Ultraviolet*

An Alkaline Solution of Potassium Chromate as a Transmittancy Standard in the Ultraviolet*

Molecular Weight and Quaternary Structure of Yeast l‐Lactate Dehydrogenase (Cytochrome b₂)

ANALYSIS OF PLANT EXTRACTS FOR CHLOROPHYLLS a AND b BY A PHOTOELECTRIC SPECTROPHOTOMETRIC METHOD

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Photoelectric Spectrophotometry. An Apparatus for the Ultra-violet and Visible Spectral Regions: Its Construction, Calibration, and Application to Chemical Problems

Cited by 128 publications

References 6 publications

An Alkaline Solution of Potassium Chromate as a Transmittancy Standard in the Ultraviolet*

An Alkaline Solution of Potassium Chromate as a Transmittancy Standard in the Ultraviolet*

Molecular Weight and Quaternary Structure of Yeast l‐Lactate Dehydrogenase (Cytochrome b2)

ANALYSIS OF PLANT EXTRACTS FOR CHLOROPHYLLS a AND b BY A PHOTOELECTRIC SPECTROPHOTOMETRIC METHOD

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Product

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Molecular Weight and Quaternary Structure of Yeast l‐Lactate Dehydrogenase (Cytochrome b₂)