Photoelectrochemical Detection of L‐Cysteine with a Covalently Grafted ZnTAPc‐Gr‐based Probe

Abstract

L‐cysteine plays a vital role in organisms, and is an important biomarker for many pathological diseases that seriously affect human health. In the study, a photoelectrochemical (PEC) probe with zinc‐tetramine phthalocyanine covalently grafted to graphene oxide (ZnTAPc‐Gr) was developed for L‐cysteine detection. Graphene oxide (GO) with carboxyl was used to immobilize zinc‐tetramine phthalocyanine (ZnTAPc) with amidogen (a graft structure formed by an amide covalent bond), which could firmly immobilize ZnTAPc, thereby improving the photoelectrochemical performance and stability. L‐cysteine molecule, an electron acceptor, was specifically recognized by the PEC probe, exhibiting a decrease in the photocurrent signal. Under the optimal experimental conditions, the fabricated PEC probe exhibited excellent performance in L‐cysteine analysis within a linear range of 0.25–113 μM and a detection limit of 11.4 nM. The PEC probe showed high sensitivity, selectivity, and stability. The method described herein provides an effective strategy for PEC probe construction for L‐cysteine detection, and can also serve as a promising PEC platform for the analyses of other small molecules.

Artemisinin: a novel chiral electrochemiluminescence luminophore-assisted enantiospecific recognition and mechanism identification

“…The calculated limit of detection (LOD) is located at a very low value of 3.7 nM (S/N = 3). [46][47][48] In contrast, the quenching effect of D-Cys on the artemisinin ECL system was not signicant in the range of 0.01-0.30 mM (Fig. 4D).…”

Chemiluminescence‐driven photoelectrochemical sensor: A mini review

“…The continual expansion in photoactive materials and sensing strategies has significantly propelled the rapid progress in PEC analysis [14–17]. As a result of these advancements, numerous PEC sensing platforms have been successfully established and applied for detecting various analytes, including ions [18], small molecules [19–25], biomacromolecules [26–29], cells [30–33], microorganisms [34–37], and in vivo detection [38–40]. A number of key reviews have comprehensively summarized significant research advancements in PEC detection, covering topics such as photoactive materials [13, 28, 41–52], sensing modalities [53–58], and target analytes [37, 59–64].…”

In Situ Formation of Bi2MoO6-Bi2S3 Heterostructure: A Proof-Of-Concept Study for Photoelectrochemical Bioassay of l-Cysteine

“…Therefore, monitoring the content of L-Cys in human body is meaningful. Currently, some analytical methods such as high-performance liquid chromatography ( Deáková et al, 2015 ), mass spectrometry ( Li et al, 2014a ), fluorescence ( Li et al, 2019 ), colorimetry ( Song et al, 2018 ), and photoelectrochemistry (PEC) ( Peng et al, 2020 ) have been developed for L-Cys detection.…”