Photoelectrochemical DNA Biosensors

Zhao, Weiwei; Xu, Jing‐Juan; Chen, Hong‐Yuan

doi:10.1021/cr500100j

Cited by 753 publications

(437 citation statements)

References 221 publications

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“…22 Indium-doped tin oxide (ITO) is a very important material because it is both transparent and conductive. 26 ITO electrodes are used in electrical 27 and photolectrochemical 28 biosensors…”

Section: Introductionmentioning

confidence: 99%

DNA Adsorption by Indium Tin Oxide Nanoparticles

Liu

2014

Langmuir

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Indium tin oxide (ITO) is a highly important material since it is both conductive and transparent. As a result, ITO is widely used in the electronic industry and recently its application in biosensor development is also explored. We herein investigate its interaction with DNA by studying the adsorption of fluorescently labeled and single-stranded oligonucleotides onto ITO nanoparticles. The fluorescence of DNA is efficiently quenched after adsorption and the interaction between DNA and ITO NPs is dependent on the surface charge of ITO, which is controlled by pH. Adsorption is greatly enhanced at low pH.Adsorption is also influenced by the sequence and length of DNA. For its components, In2O3 adsorbs DNA more strongly while SnO2 repels DNA at neutral pH. The DNA adsorption property is an averaging result from both components. DNA adsorption is confirmed to be mainly by the phosphate backbone via displacement experiments using free phosphate or DNA bases. Lastly, DNA induced DNA desorption by forming duplex DNA is demonstrated on ITO, while the same reaction is more difficult to achieve on other metal oxides including CeO2, TiO2 and Fe3O4.3

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Section: Introductionmentioning

confidence: 99%

DNA Adsorption by Indium Tin Oxide Nanoparticles

Liu

2014

Langmuir

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“…From the thermodynamics point of view, Ag or silver nanoparticles and crystal are not stable or metastable. Gold nanoparticles AuNP(s) have excellent catalytic ability, good intrinsic conductivity, and optical ability, which have been extensively studied in many fields such as catalysis, electroanalysis, and nanodevices [38][39][40][41]. Thus, the combination of AuNPs with manganese oxide is beneficial to enhance the performance of electrochemical sensors and avoid the disadvantages of Ag nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

The Hybrid of Gold Nanoparticles and 3D Flower‐like MnO₂ Nanostructure with Enhanced Activity for Detection of Hydrogen Peroxide

2017

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3D Flower-like manganese dioxide (MnO 2 ) nanostructure with the ability of catalysis for hydrogen peroxide (H 2 O 2 ) and super large area that can support gold nanoparticles (AuNPs) with enhanced activity of electron transfer have been developed. The nanostructure of hybrids was prepared by directly mixing citric-capped AuNPs and 3-aminopropyltriethoxysilane (3-APTES)-capped nano-MnO 2 using an electrostatic adsorption strategy. The Au-MnO 2 composite was extensively characterized by scanning electron microscope (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), the Brunauer-Emmett-Teller (BET) method and X-ray photoemission spectroscopy (XPS). Electrochemical properties were evaluated through cyclic voltammetry (CV) and amperometric method. The prepared sensor showed excellent electrochemical properties towards H 2 O 2 with a wide linear range from 2.5 3 10 À31.39 mM and 3.89~13.89 mM. The detection limit is 0.34 mM (S/N = 3) with the sensitivities of 169.43 mA mM À1 cm À2 and 55.72 mA mM À1 cm À2 . The detection of real samples was also studied. The result exhibited that the prepared sensor can be used for H 2 O 2 detection in real samples.

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“…For the inherent virtues and advantages, PEC biosensors are extensively harnessed in DNA detection and have scored great achievements [11]. The detection of DNA by PEC biosensors is generally based on the immobilization of short oligonucleotide capture probes onto a solid support to form a biorecognition layer and then hybridized with the target nucleic acids [12,13].…”

Section: Introductionmentioning

confidence: 99%

A photoelectrochemical biosensor for determination of DNA based on flower rod-like zinc oxide heterostructures

et al. 2017

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The work describes a photoelectrochemical (PEC) biosensor for the detection of DNA. It employs oriented hierarchical ZnO flower-rod architectures (ZnO FRs) and DNA dendrimers. ZnO FRs act as photoactive material that yields a photocurrent of up to 23 μA. The photogenerated electron transfer is inhibited once the probe DNA and the target DNA oligonucleotides hybridize, and this results in a reduced photocurrent. The use of DNA dendrimers with scores of DNA branches further amplifies the signal of the PEC biosensor. The PEC sensor displays a response that is linear in the DNA concentration range from 10 fM to 0.1 μM with a detection limit of 3.7 fM (at S/N = 3). The sensor was applied to the determination of DNA in human serum samples and was found to work with acceptable accuracy. Due to the use of ZnO FRs and DNA dendrimers, the assay is highly sensitive, rapid, and repeatability.

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Photoelectrochemical DNA Biosensors

Cited by 753 publications

References 221 publications

DNA Adsorption by Indium Tin Oxide Nanoparticles

DNA Adsorption by Indium Tin Oxide Nanoparticles

The Hybrid of Gold Nanoparticles and 3D Flower‐like MnO₂ Nanostructure with Enhanced Activity for Detection of Hydrogen Peroxide

A photoelectrochemical biosensor for determination of DNA based on flower rod-like zinc oxide heterostructures

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Photoelectrochemical DNA Biosensors

Cited by 753 publications

References 221 publications

DNA Adsorption by Indium Tin Oxide Nanoparticles

DNA Adsorption by Indium Tin Oxide Nanoparticles

The Hybrid of Gold Nanoparticles and 3D Flower‐like MnO2 Nanostructure with Enhanced Activity for Detection of Hydrogen Peroxide

A photoelectrochemical biosensor for determination of DNA based on flower rod-like zinc oxide heterostructures

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The Hybrid of Gold Nanoparticles and 3D Flower‐like MnO₂ Nanostructure with Enhanced Activity for Detection of Hydrogen Peroxide