Photoelectrochemical immunosensor for carcinoembryonic antigen detection-an attempt for early cancer screening

Mao, Chunling; Wu, Lei; Wen, Yi; Tang, Ximing; Huang, Zheng; Zhao, Longshan

doi:10.1016/j.bios.2022.114918

Cited by 21 publications

(6 citation statements)

References 35 publications

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“…Herein, a hybrid material consisting of CdTe-QDs and MIL-101(Cr) was formulated, produced, and subsequently linked to the Ab of CEA. 64 Organic–inorganic nanocomposites have revealed strong photosensitivity, high charge mobility, and effective band gap matching ability, leading to increased efficiency in separating photogenerated carriers. As the photocurrent intensity of MIL-101(Cr) varies with varying applied working voltage, a framework known as the “anodic photocurrent-CEA concentration-cathode photocurrent” system was developed.…”

Section: Immunosensing Approaches For Mof-derived Nanocompositesmentioning

confidence: 99%

Recent advancements in the specific determination of carcinoembryonic antigens using MOF-based immunosensors

Karimzadeh,

Mahmoudpour,

Rahimpour

et al. 2024

RSC Adv.

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Section: Immunosensing Approaches For Mof-derived Nanocompositesmentioning

confidence: 99%

Recent advancements in the specific determination of carcinoembryonic antigens using MOF-based immunosensors

Karimzadeh,

Mahmoudpour,

Rahimpour

et al. 2024

RSC Adv.

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“…Thus, the efficient detection of tumor biomarkers has significant clinical significance and can help in the early diagnosis of tumors, monitoring treatment effectiveness, and assessing the risk of recurrence, providing strong support for the treatment of tumor patients. For example, carcinoembryonic antigen (CEA) is a commonly used tumor biomarker, with a typical range in human serum being less than Molecules 2024, 29, 858 2 of 15 5 ng/mL [5][6][7][8]. Under normal circumstances, CEA is produced during fetal development and is only present in very small amounts in the adult body.…”

Section: Introductionmentioning

confidence: 99%

Sensitive Electrochemical Detection of Carcinoembryonic Antigen Based on Biofunctionalized Nanochannel Modified Carbonaceous Electrode

Zhou,

Wang,

et al. 2024

Molecules

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The convenient construction of carbon-based electrochemical immunosensors with high performance is highly desirable for the efficient detection of tumor biomarkers. In this work, an electrochemical immunosensor was fabricated by integrating a biofunctionalized mesoporous silica nanochannel film with a carbon-based electrode, which can enable the sensitive determination of carcinoembryonic antigen (CEA) in serum. The commonly used carbonaceous electrode, glassy carbon electrode (GCE), was employed as the supporting electrode and was pre-treated through electrochemical polarization to achieve the stable binding of a vertically ordered mesoporous silica film with amino groups (NH2-VMSF) without the use of any adhesive layer. To fabricate the immunorecognition interface, antibodies were covalently immobilized after the amino groups on the outer surface of NH2-VMSF was derivatized to aldehyde groups. The presence of amino sites within the high-density nanochannels of NH2-VMSF can facilitate the migration of negatively charged redox probes (Fe(CN)63-/4-) to the supporting electrode through electrostatic adsorption, leading to the generation of electrochemical signals. In the presence of CEA, the formation of immunocomplexes on the recognitive interface can reduce the electrochemical signal of Fe(CN)63-/4- on the supporting electrode. Based on this principle, the sensitive electrochemical detection of CEA was achieved. CEA can be determined to range from 0.01 ng mL−1 to 100 ng mL−1 with a limit of detection of 6.3 pg mL−1. The fabricated immunosensor exhibited high selectivity, and the detection of CEA in fetal bovine serum was achieved.

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“…12 Therefore, photoactive materials such as photoelectric semiconductors were introduced to realize a dual−anodic and cathodic−photocurrent detection strategy. 13 Except for semiconductors, the metal− organic framework (MOF) is another outstanding material 14 due to many merits, e.g., high specific surface area, structural stability, large volume, and surface porosity. 15 The integration of MOF, e.g., MIL-101(Cr), 16 into a photoactive composite enhances the electrode's light-receiving area and facilitates the formation of heterojunctions, which promote efficient electron−hole separation and intensify photocurrent signals.…”

Section: ■ Introductionmentioning

confidence: 99%

“…For two analytes, the one electrode-based PEC has always suffered from signal bias induced by the measured multiple substances . Therefore, photoactive materials such as photoelectric semiconductors were introduced to realize a dual–anodic and cathodic–photocurrent detection strategy . Except for semiconductors, the metal–organic framework (MOF) is another outstanding material due to many merits, e.g., high specific surface area, structural stability, large volume, and surface porosity .…”

Section: Introductionmentioning

confidence: 99%

Versatile Metal–Organic Framework Incorporating Ag₂S for Constructing a Photoelectrochemical Immunosensor for Two Breast Cancer Markers

Zhong,

Ding,

Man

et al. 2024

Anal. Chem.

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Breast cancer poses the significance of early diagnosis and treatment. Here, we developed an innovative photoelectrochemical (PEC) immunosensor characterized by high-level dual photocurrent signals and exceptional sensitivity. The PEC sensor, denoted as MIL&Ag 2 S, was constructed by incorporating Ag 2 S into a metal−organic framework of MIL-101(Cr). This composite not only enhanced electron−hole separation and conductivity but also yielded robust and stable dual photocurrent signals. Through the implementation of signal switching, we achieved the combined detection of cancer antigen 15-3 (CA15-3) and carcinoembryonic antigen (CEA) with outstanding stability, reproducibility, and specificity. The results revealed a linear range for CEA detection spanning 0.01−32 ng/mL, with a remarkably low detection limit of 0.0023 ng/mL. Similarly, for CA15-3 detection, the linear range extended from 0.1 to 320 U/mL, with a low detection limit of 0.014 U/mL. The proposed strategy introduces new avenues for the development of highly efficient, cost-effective, and user-friendly PEC sensors. Furthermore, it holds promising prospects for early clinical diagnosis, contributing to potential breakthroughs in medical detection and ultimately improving patient outcomes.

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Photoelectrochemical immunosensor for carcinoembryonic antigen detection-an attempt for early cancer screening

Cited by 21 publications

References 35 publications

Recent advancements in the specific determination of carcinoembryonic antigens using MOF-based immunosensors

Recent advancements in the specific determination of carcinoembryonic antigens using MOF-based immunosensors

Sensitive Electrochemical Detection of Carcinoembryonic Antigen Based on Biofunctionalized Nanochannel Modified Carbonaceous Electrode

Versatile Metal–Organic Framework Incorporating Ag₂S for Constructing a Photoelectrochemical Immunosensor for Two Breast Cancer Markers

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Photoelectrochemical immunosensor for carcinoembryonic antigen detection-an attempt for early cancer screening

Cited by 21 publications

References 35 publications

Recent advancements in the specific determination of carcinoembryonic antigens using MOF-based immunosensors

Recent advancements in the specific determination of carcinoembryonic antigens using MOF-based immunosensors

Sensitive Electrochemical Detection of Carcinoembryonic Antigen Based on Biofunctionalized Nanochannel Modified Carbonaceous Electrode

Versatile Metal–Organic Framework Incorporating Ag2S for Constructing a Photoelectrochemical Immunosensor for Two Breast Cancer Markers

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Versatile Metal–Organic Framework Incorporating Ag₂S for Constructing a Photoelectrochemical Immunosensor for Two Breast Cancer Markers