Photoelectrochemical Studies of the TiO&lt;sub&gt;2&lt;/sub&gt; Nanocrystalline Electrode Sensitized by Novel Cyanine Dye

Cyanine dyes, as used in super‐resolution fluorescence microscopy, undergo light‐induced “blinking”, enabling localization of fluorophores with spatial resolution beyond the optical diffraction limit. Despite a plethora of studies, the molecular origins of this blinking are not well understood. Here, we examine the photophysical properties of a bio‐conjugate cyanine dye (AF‐647), used extensively in dSTORM imaging. In the absence of a potent sacrificial reductant, light‐induced electron transfer and intermediates formed via the metastable, triplet excited state are considered unlikely to play a significant role in the blinking events. Instead, it is found that, under conditions appropriate to dSTORM microscopy, AF‐647 undergoes reversible photo‐induced isomerization to at least two long‐lived dark species. These photo‐isomers are characterized spectroscopically and their interconversion probed by computational means. The first‐formed isomer is light sensitive and transforms to a longer‐lived species in modest yield that could be involved in dSTORM related blinking. Permanent photobleaching of AF‐647 occurs with very low quantum yield and is partially suppressed by the anaerobic redox buffer.

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Photo‐isomerization of the Cyanine Dye Alexa‐Fluor 647 (AF‐647) in the Context of dSTORM Super‐Resolution Microscopy

Karlsson

Laude

Hall

et al. 2019

Chemistry A European J

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Photovoltaics literature survey (no. 34)

Richards

2004

Progress in Photovoltaics

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Near-infrared chemodosimetric probes based on heptamethine cyanine dyes for the “naked-eye” detection of cyanide in aqueous media

Qiu

Chen

2017

Journal of Luminescence

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Photoelectrochemical Studies of the TiO<sub>2</sub> Nanocrystalline Electrode Sensitized by Novel Cyanine Dye

Cited by 4 publications

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Photo‐isomerization of the Cyanine Dye Alexa‐Fluor 647 (AF‐647) in the Context of dSTORM Super‐Resolution Microscopy

Photo‐isomerization of the Cyanine Dye Alexa‐Fluor 647 (AF‐647) in the Context of dSTORM Super‐Resolution Microscopy

Photovoltaics literature survey (no. 34)

Near-infrared chemodosimetric probes based on heptamethine cyanine dyes for the “naked-eye” detection of cyanide in aqueous media

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