Photoencapsulation of Bone Morphogenetic Protein-2 and Periosteal Progenitor Cells Improve Tendon Graft Healing in a Bone Tunnel

Chen, Chih-Hwa; Liu, Hsia‐Wei; Tsai, Ching-Lin; Yu, Chao‐Lan; Lin, I-Hsuan; Hsiue, Ging‐Ho

doi:10.1177/0363546507311098

Cited by 74 publications

(70 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This differs from the intra-articular tendon-bone healing required for ACL reconstruction. However, this model of graft implantation has been wellestablished in several tendon-bone healing studies [33,34], and it is feasible for preliminary research of a new graft coating. Second, the thickness of the HPC coating was difficult to control, which may have influenced the effect of HPC coating on the graft-bone healing in the bone tunnel.…”

Section: Discussionmentioning

confidence: 99%

A new strategy to enhance artificial ligament graft osseointegration in the bone tunnel using hydroxypropylcellulose

Yang

Jiang

et al. 2012

International Orthopaedics (SICOT)

View full text Add to dashboard Cite

Purpose The aim of this study was to determine whether hydroxypropylcellulose (HPC) coating of polyethylene terephthalate (PET) artificial ligaments enhances graft osseointegration in the bone tunnel. Methods Thirty New Zealand white rabbits underwent artificial ligament graft transplantation in the bilateral proximal tibia tunnels. One limb was implanted with an HPC-coated PET graft, and the contralateral limb was implanted with a non-HPC-coated PET graft as a control. The rabbits were then randomly sacrificed at weeks four and eight after surgery for biomechanical testing, histological examination, and histomorphometric and real-time polymerase chain reaction analysis.

show abstract

Section: Discussionmentioning

confidence: 99%

A new strategy to enhance artificial ligament graft osseointegration in the bone tunnel using hydroxypropylcellulose

Yang

Jiang

et al. 2012

International Orthopaedics (SICOT)

View full text Add to dashboard Cite

show abstract

“…We are not sure whether the observed higher osteogenic response in TDSCs compared with BMSCs is due to the higher BMPR-IA, BMPR-IB and BMPR-II expression. However, the roles of these receptors in mediating BMP-2-induced osteogenic differentiation of stem cells have been reported in the literature [16,22,23]. Overexpression of dominant-negative BMPR-1A, but not BMPR-1B, in mesenchymal progenitors C3H10T1/2 reduced osteogenesis and osteogenic marker expression [osteocalcin, osteopontin, parathyroid hormone/ PTH-related peptide (PTH/PTHr) Preceptor, Runx2] induced by BMP-2 overexpression [22].…”

Section: Discussionmentioning

confidence: 99%

“…BMP-12 and BMP-13, which are tenogenic, activated the same sets of receptors as BMP-2 [16,23]. Besides BMP-2, BMPs such as BMP-4 and BMP-7 are osteochondrogenic and have been reported to play important roles in bone and TBJ regeneration [30,31].…”

Section: Discussionmentioning

confidence: 99%

“…Bone morphogenic protein 2 (BMP-2) is an osteogenic growth factor that has been used in many studies to augment bone and TBJ repair [12][13][14][15][16][17][18]. Osteogenic responses of stem cells from different sources to BMP-2 can be different.…”

Section: Introductionmentioning

confidence: 99%

“…Because of the importance of BMP-2 on stem cells in TBJ engineering and tendon pathology, this study aimed to compare the osteogenic response of TDSCs and BMSCs to BMP-2 in vitro. The expression of BMP receptors (BMPR-IA, BMPR-IB, BMP-II) were also examined in these two cell types to explain any differences in BMP-2-induced osteogenic differentiation that might be observed, as these receptors have been reported to mediate BMP-2 osteogenic response [16,22,23]. This study investigated whether TDSCs would be an attractive new source for therapeutic stem-cell-based TBJ repair and offers new insights into the role of TDSCs in tendon physiology/pathology.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Higher BMP receptor expression and BMP-2-induced osteogenic differentiation in tendon-derived stem cells compared with bone-marrow-derived mesenchymal stem cells

Rui

Lui

Lee

et al. 2011

International Orthopaedics (SICOT)

View full text Add to dashboard Cite

Purpose Surgical reattachment of tendon to bone often fails due to regeneration failure of the specialised tendon-bone junction (TBJ). The use of mesenchymal stem cells for TBJ regeneration has been reported with promising results. Tendonderived stem cells (TDSCs) with high proliferative and multilineage differentiation potential have been isolated. As stem cells residing in tendons, TDSCs can be considered a new cell source for TBJ repair. Bone morphogenic protein 2 (BMP-2) is a potent osteogenic factor with roles in normal bone healing and pathological ectopic bone formation in soft tissues. The use of BMP-2 to promote TBJ repair has been well reported. This study aimed to compare TDSCs to the gold standard bonemarrow-derived mesenchymal stem cells (BMSCs) with respect to osteogenic response to BMP-2 in vitro. Method The clonogenicity and multi-differentiation potential of TDSCs and BMSCs were identified by colony-formingunit assay, osteogenic, adipogenic and chondrogenic differentiation assays. Their osteogenic response to BMP-2 in vitro was examined by alkaline phosphatase (ALP) cytochemical staining, ALP activity assay and Alizarin red S staining of calcium nodule formation. Messenger RNA (mRNA) and BMP receptor (types IA, IB and II) protein expression were examined by quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR) and Western blotting. Results Our results showed that both TDSCs and BMSCs exhibited stem cell properties, including clonogenicity and multi-differentiation potential. TDSCs expressed higher mRNA and protein levels of BMP receptors IA, IB and II. They also exhibited higher osteogenic differentiation with and without BMP-2 stimulation compared with BMSCs. Conclusions TDSCs with/without BMP-2 might be an attractive source for TBJ repair compared with BMSCs.

show abstract

The use of brushite calcium phosphate cement for enhancement of bone‐tendon integration in an anterior cruciate ligament reconstruction rabbit model

et al. 2008

View full text Add to dashboard Cite

This study was designed to investigate the osteoconductivity and bioresorption of brushite calcium phosphate cement (CPC) in bone-tendon interface healing after anterior cruciate ligament (ACL) reconstruction. Surgical reconstruction using grafted tendon in bone tunnel was performed bilaterally in 28 skeletal mature rabbits. Brushite CPC was implanted between grafted tendon and bone tunnel of one limb with the contralateral one as the control. A batch of 14 rabbits was sacrificed at 6 and 12 weeks, respectively, after surgery. At each time point, six rabbits were used for micro-CT and subsequent histological examinations, whereas the remaining eight rabbits were used for pull-out testing. The components of brushite CPC-dicalcium phosphate dihydrate matrix degraded rapidly with beta-tricalcium phosphate granules left for guiding new bone formation. Brushite CPC augmented the peri-tendon bone volume and promoted bone growth into the healing interface. The ultimate strength and stiffness of the graft-tunnel complexes on experimental side was higher than that of the control by 117% and 102%, respectively, at 6 weeks postoperatively (p < 0.05 for both). The use of brushite CPC caused a paradigm shift in failure mode from intra-tunnel to intra-articular portion at 12 weeks postoperatively (p = 0.013). Brushite CPC significantly enhanced the bone-tendon integration after ACL reconstruction, which provided a scientific basis for clinical application.

show abstract

Photoencapsulation of Bone Morphogenetic Protein-2 and Periosteal Progenitor Cells Improve Tendon Graft Healing in a Bone Tunnel

Abstract: Novel technologies, such as those described in this study, including photopolymerization and tissue engineering, may provide minimally invasive therapeutic procedures via arthroscopy to enhance biological healing after reconstruction of the anterior cruciate ligament.

Cited by 74 publications

References 43 publications

A new strategy to enhance artificial ligament graft osseointegration in the bone tunnel using hydroxypropylcellulose

A new strategy to enhance artificial ligament graft osseointegration in the bone tunnel using hydroxypropylcellulose

Higher BMP receptor expression and BMP-2-induced osteogenic differentiation in tendon-derived stem cells compared with bone-marrow-derived mesenchymal stem cells

The use of brushite calcium phosphate cement for enhancement of bone‐tendon integration in an anterior cruciate ligament reconstruction rabbit model

Contact Info

Product

Resources

About